Micromonospora and application thereof

A technology of Micromonas and Micromonas, applied in bacteria, water/sludge/sewage treatment, biochemical equipment and methods, etc., to achieve the effect of wide degradation spectrum, protection of ecological environment and good treatment effect

Active Publication Date: 2014-10-29
ENVIRONMENTAL SCI RES & DESIGN INST OF ZHEJIANG PROVINCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although in Actinomyces and Micromonospora all have the bacterial strain (Biodegradation of cis-1,4-polyisoprene rubbers by distinct Actinomycetes: Microbial strategies and detailed surface analysis.Appl.Environ.Microbiol,2000,66(4): 1639-1645), but there is no report that Micromonospora marine can degrade terpene compounds

Method used

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  • Micromonospora and application thereof
  • Micromonospora and application thereof
  • Micromonospora and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Obtaining and identification of actinomycete Micromonospora orange (Micromonospora aurantiaca) ZHY1-5

[0042] 1. Acquisition and identification of strains

[0043] (1) Preliminary screening: Take 10g of activated sludge from the wastewater treatment system for producing terpene resins, inoculate it in an inorganic salt liquid medium containing 200mg / L α-pinene, enrich and cultivate it on a shaking table at 30°C and 160rpm for 5 days, Take 1 mL of the enriched bacteria liquid and inoculate it into a new liquid medium containing the same concentration of α-pinene, and then enrich it twice. Then take 1mL of the bacterial solution and carry out gradient dilution with sterile water, spread it on the inorganic salt medium containing 200mg / L α-pinene, incubate the plate upside down at 30°C, observe the growth of a single colony on the plate, and then pick different shapes, Colonies with the same color were observed under a microscope, and the colonies with the same...

Embodiment 2

[0057] Example 2 Degradation of different compounds by bacterial strain ZHY1-5 in inorganic salt-based liquid medium

[0058] (1), pick a ring of bacteria from the plate and inoculate it in a liquid growth medium (liquid LB medium), cultivate it to the logarithmic growth phase at 35° C., 160 rpm, collect the bacteria by centrifugation at 12000 rpm, wash 3 times with sterile water, Adjust OD after resuspension in sterile water 600 To 1.5, inoculate 3mL into the inorganic salt liquid medium with 200mg / L of various organic matter as the sole carbon source respectively, put 100mL medium in a 250mL shaker flask, culture at 30°C, 180rpm shaker for 48h, then take samples for measurement For the degradation rate of different substrates, the blank without inoculation was used as the control.

[0059] (2) The organic pollutants added in the inorganic salt medium are BTEX benzene series (benzene, toluene, ethylbenzene, o-xylene, m-xylene, p-xylene), petroleum ether, cyclohexane, n-hexen...

Embodiment 3

[0065] The treatment effect of embodiment 3 bacterial strain ZHY1-5 to terpene resin production wastewater

[0066] Pick a ring of bacteria from the plate and inoculate it in a liquid growth medium (liquid LB medium), cultivate it to the logarithmic growth phase at 35°C, 160rpm, collect the bacteria by centrifugation at 12000rpm, wash 3 times with sterile water, weigh OD after suspension 600 to 1.5.

[0067] Take the activated sludge of the sewage treatment plant of the terpene resin production enterprise and the effluent of the sewage treatment plant. The COD of the effluent is about 563.44mg / L. Put 250mL of the effluent of the sewage treatment plant in a 500mL triangular bottle, and the amount of activated sludge is 30%. Dosing, inoculate 1%, 2%, and 3% fresh strains respectively, control without adding bacteria, culture at 30°C, 180rpm for 48h, let stand, pour off 100mL of supernatant, then add the same volume of effluent, 30°C, Cultivate at 180rpm for 48h, and measure th...

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Abstract

The invention discloses micromonospora and an application thereof. The micromonospora is named as micromonospora aurantiaca in China, has the strain number of ZHY1-5, is separated from activated sludge from a sewage treatment plant for producing terpene resin and is preserved with the number of CGMCC No.8215 in China General Microbiological Culture Collection Center located in Institute of Microbiology, Chinese Academy of Sciences in No.3, No.1 yard, Beichen west Road, Chaoyang district, Beijing. The micromonospora is relatively good in effect when being applied to degradation of alpha-terpene and remarkable in degradation effect for alpha-pinene; and the micromonospora capable of discharging is also applied to treatment of terpene compound synthesis wastewater and chemical benzene series compounds and has a relatively good treatment effect for the terpene compound synthesis wastewater.

Description

technical field [0001] The present invention relates to the technical field of degrading α-terpene compounds by a strain of micromonas actinomycetes, in particular to the enrichment culture, screening, separation and verification of bacterial strain substrate spectrum, and the process method for treating terpene compound synthesis wastewater . Background technique [0002] As important chemical intermediates, terpenes are widely used in wood processing, spice manufacturing, pharmaceutical, chemical and other industries. The wastewater produced in the production process of these industries contains a large amount of organic substances, high COD, high toxicity, and difficult to biodegrade. It is a typical high-concentration and refractory organic wastewater. [0003] Terpene compounds have various structures, most of which are volatile and form aerosol particles in the atmosphere, which can easily cause respiratory diseases and endanger people's health. Some terpene compound...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/28C02F3/34C12R1/29C02F101/32
Inventor 王慧荣徐佳佳梅荣武韦彦斐李明智任旭峰
Owner ENVIRONMENTAL SCI RES & DESIGN INST OF ZHEJIANG PROVINCE
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