Multicopper oxidase mutant with improved salt tolerance

A multi-copper oxidase and mutant technology, applied in the field of bioengineering, can solve problems such as reducing protein content, complex relationships, and changes in fermentation systems
CN110106153AActive Publication Date: 2019-08-09JIANGNAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
JIANGNAN UNIV
Publication Date
2019-08-09

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Abstract

The invention discloses a multicopper oxidase mutant with improved salt tolerance. Through site-specific mutagenesis, the 317th-site threonine of a wild multicopper oxidase WT is mutated into asparaginate, the 386th-site leucine is mutated into tyrosine, the 427th-site serine is mutated into glutamate, and the mutant T317N-L386Y-S427E is formed. The tolerance of the mutant T317N-L386Y-S427E to 6%,9%, 12%, 15% and 18% NaCl (W / V) is improved compared with the WT.
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Description

technical field

[0001] The invention relates to a multi-copper oxidase mutant with improved salt tolerance, in particular to a multi-copper oxidase mutant derived from Bacillus amyloliquefaciens with improved salt tolerance, and belongs to the technical field of bioengineering. Background technique

[0002] Biogenic amine is a general term for a class of biologically active low-molecular-weight nitrogen-containing organic compounds, which are normal physiologically active substances in living organisms. The appropriate amount of biogenic amines synthesized by the human body can help maintain the normal physiological functions of the body. However, excessive intake of biogenic amines through food can cause allergic reactions such as headache, nausea, heart palpitations, changes in blood pressure, and respiratory disorders, which can be life-threatening in severe cases. .

[0003] Biogenic amines are widely found in fermented foods rich in protein, such as dairy products, aqu...

Claims

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