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A kind of amine oxidase acao, preparation method and application

A technology for separation and purification of amine oxidase, which is applied in the field of agricultural biology, can solve the problems of reduced food quality and incomplete degradation of toxins by chemical methods, and achieve the effect of reducing harm

Active Publication Date: 2022-08-05
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Currently known fumonisins degradation methods mainly include physical degradation, chemical degradation and biological degradation. Some toxins are not fully degraded by physical methods, and chemical methods are likely to cause food quality degradation. Both physical and chemical methods have some shortcomings.

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  • A kind of amine oxidase acao, preparation method and application
  • A kind of amine oxidase acao, preparation method and application
  • A kind of amine oxidase acao, preparation method and application

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Embodiment Construction

[0025] The embodiments of the present invention will be described in detail below. It should be noted that the embodiments are descriptive, not restrictive, and cannot limit the protection scope of the present invention.

[0026] The raw materials used in the present invention are conventional commercial products unless otherwise specified; the methods used in the present invention are conventional methods in the art unless otherwise specified.

[0027] An amine oxidase ACAO, the amino acid sequence of the amine oxidase ACAO is SEQ ID NO.1.

[0028] The above-mentioned preparation method of amine oxidase ACAO, the steps are as follows:

[0029] (1) adopting the means of molecular biology to recombine the gene encoding the amine oxidase ACAO into a vector and then transform a host cell to obtain a recombinant strain;

[0030] (2) Cultivate the recombinant strain to induce the expression of amine oxidase ACAO;

[0031] (3) amine oxidase ACAO obtained by separation and purifica...

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Abstract

The present invention relates to an amine oxidase ACAO, and the amino acid sequence of the amine oxidase ACAO is SEQ ID NO.1. The amine oxidase ACAO of the present invention has a degradation rate of 100% to HFB1; the amine oxidase ACAO of the present invention has excellent properties, and the enzyme can be applied to industries such as agriculture, feed and food to reduce the harm of fumonisin FB1 to animal and human health .

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology, in particular to an amine oxidase ACAO, a preparation method and an application. Background technique [0002] Fumonisins (also known as fumonisins) are mycotoxins discovered in 1989 by fungi such as Fusarium proliferatum, Fusarium moniliforme and Fusarium verticillioides The resulting water-soluble metabolites are a class of structurally similar diester compounds composed of different kinds of polyhydric alcohols and glycerol. Fumonisins mainly exist in animal feed raw materials, which can cause acute poisoning in some livestock, and are potentially carcinogenic to most poultry. At the same time, there is the possibility of human infection, so how to degrade fumonisins to the greatest extent becomes a Another research hotspot after aflatoxin. my country has a vast territory, and the feed in some areas is prone to produce mycotoxins such as fumonisins due to environmental factors. [...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/06C12N15/70C12N1/21A23K20/189A23L33/10C12R1/19
CPCC12N9/0022C12N15/70A23K20/189A23L33/10
Inventor 李中媛张同存夏忠强罗学刚宋亚囝
Owner TIANJIN UNIV OF SCI & TECH
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