31 results about "Sulfhydryl oxidase" patented technology

The invention belongs to the field of food additives, and particularly discloses a high-efficiency bread flour modifying agent. The high-efficiency bread powder modifying agent is prepared by taking alpha-amylase, thiol oxidase, hemicellulase, lipase, azodicarbonamide, glycerol monostearate, calcium stearoyl lactate, diacetyl tartaric acid monoglyceride and modified starch as raw materials; and uniformly mixing the raw materials in certain weight percentage. The high-efficiency bread flour modifying agent has simple preparation, is green and safe, can effectively improve the processing performance of bread flour, obviously increases the volume of bread, improves internal structures, makes the surface of the bread smooth and elastic, and can prolong quality guarantee period of the bread.

The present invention is directed to the treatment of reactive thiol groups (—SH) found in thiol-containing flavor compounds by a highly selective enzymatic conversion into aroma-active disulides compounds using sulfhydryl oxidase.

Biomarkers for pre-Diabetes, Diabetes and / or a Diabetes related conditions, and methods of their use, including the biomarkers in Tables 1 and 2 such as peroxiredoxin-2, complement C1q subcomponent subunit B, sulfhydryl oxidase 1 and apolipoprotein A-IV.

The present invention relates to a method for producing a protein of interest containing one or more disulfide bonds in its native state. The method comprises that a prokaryotic host cell is genetically engineered to express the protein of interest and a sulfhydryl oxidase in the cytoplasm of the host cell. The protein of interest is formed in a soluble form and contains disulfide bonds due to the presence of the sulfhydryl oxidase in the cytoplasm of said host cell. The present invention relates also to a prokaryotic host cell and a vector system for producing a protein of interest containing natively folded disulfide bonds.

The invention provides an application of a combination of a sulfhydryl oxidase 1 agonist and sorafenib in preparation of liver cancer treatment cells. The sulfhydryl oxidase 1 agonist comprises lentivirus for overexpression of sulfhydryl oxidase 1 and recombinant sulfhydryl oxidase 1 protein; firstly, lentivirus for overexpression of sulfhydryl oxidase 1 is constructed, and then target cells are transfected; and the dosage forms of the sulfhydryl oxidase 1 agonist comprise an oral preparation, an injection or a sustained release preparation and the like. The lentivirus for overexpression of QSOX1 and sorafenib are used for acting on liver cancer cells at the same time, QSOX1 can inhibit the activity of the liver cancer cells, the GSH content can be more obviously reduced, the free ferrousions in the cells and the lipid peroxidation level are increased, and thus cell ferroptosis is promoted; and therefore, the lentivirus for overexpression of QSOX1 is combined with sorafenib, so that the inhibition effect on tumor growth is promoted synergistically, and a stronger killing effect is achieved.

The invention provides a diesel oil improver component containing biological enzyme, which comprises cetane number improver and biological enzyme, wherein the weight part ratio of the cetane number improver to the biological enzyme is 1:(0.01-1); the biological enzyme is the mixture of sulpho enzyme, alcohol oxidase, aldehyde oxidase, amine oxidase, sulfhydryl oxidase, methyl mercaptan oxidase, methyl naphthoquinone oxidase, peroxidase, ferrochelatase, decarboxylase, aldolase, oxyacid lyase, demethylase, alcohol dehydratase, acid dehydratase, esters dehydratase, desulfhydrase, desulfurization methyl enzyme, cobalt chelating enzyme and magnesium chelating enzyme. According to the diesel oil improver component containing the biological enzyme, provided by the invention, the high speed catalytic performance can be realized under the temperate environment through utilizing properties of the biological enzyme, and the component is combined with the conventional cetane number improver for use, the catalytic combustion efficiency of the cetane number improver in diesel oil is greatly improved, and the combustion performance of the diesel oil is further improved.

The present invention provides methods for tumor treatment by administering an inhibitor of quiescin sulfhydryl oxidase 1 (QSOX1), compositions comprising such inhibitors, and methods for identifying such inhibitors.

The invention relates to the enzymatic treatment of a proteinaceous substratewith a first enzyme, such as a sulfhydryl oxidase. The first enzyme removes enzyme inhibitors, such as free thiols, present in the proteinaceous substrate. The removal of the inhibitory compounds in the substrateallows for an effective enzymatic action of a second enzyme such as protein cross-linking of the protein present in the substrate by tyrosinase enzymes.

Disclosed is as a biomarker useful in early diagnosis of lung cancer, at least one protein selected from the group including Quescin-sulfhydryl oxidase 1, Fibrillin-1, Isoform A of Lamin-A / C, Latent-transforming growth factor beta-binding protein 2, Galectin-1, highly similar to Dickkopf-related protein 3, Isoform Al—B of Heterogeneous nuclear ribonucleoprotein Al, 14-3-3 protein epsilon, Stanniocalcin-2, Cystatin-C, Isoform 1 of Connective tissue growth factor, Profilin-1, Isoform 1 of Extracellular matrix protein 1, Histone H2B type 2-E, Kinesin-like protein KIF26A, Zinc finger protein 516, and Isoform 1 of A-kinase anchor protein 9.

An antibody comprising an antigen recognition domain exhibiting species cross reactivity to human QSOX1 and murine QSOX1 is disclosed. Methods of producing the antibody, pharmaceutical compositions comprising the antibody and methods of using the antibody for treating medical conditions are also disclosed.

The invention relates to a method for preparing sodium 3,3'-dithiodipropane sulfonate by using a biological enzyme method. The method comprises the following steps: S1, preparing a sodium 3-mercaptopropanesulphonate solution with the concentration of 15 to 30 percent; S2, selecting sulfhydryl oxidase as a catalyst and adding the sulfhydryl oxidase into the solution prepared in the step S1, and enabling the solution to continuously pass through air; meanwhile adjusting a pH value of the solution to 6.5 to 7.5, and carrying out aqueous-phase catalytic oxidation reaction; S3, after the catalytic reaction is finished, filtering the solution by using an ultrafiltration membrane, removing enzyme protein in the solution and carrying out decolorization; S4, carrying out concentrated dehydration on filtrate obtained in the step S3 by a thickener, thus obtaining the sodium 3,3'-dithiodipropane sulfonate. The method disclosed by the invention has the advantages of mild conditions, short process flow, simple steps, no pollution to the environment, low energy consumption and high efficiency.

The invention provides a CHO cell strain modified based on CRISPR / Cas9 gene editing and a preparation method thereof. Sulfhydryl oxidase (HsQSOX1b) related to disulfide bond folding and survivin related to apoptosis are selected as target genes and designed as heterogenous expression cassettes EC#1 and EC#2, and a novel CRISPR / Cas9 gene editing technology is used for integrating the heterogenous expression cassettes into a specific genetic locus of a CHO cell. According to the CHO cell strain modified based on CRISPR / Cas9 gene editing and the preparation method thereof, the CRISPR / Cas9 gene editing technology is used, human HsQSOX1b and survivin genes accurately edit a CHO-K1 cell, a rapid and efficient host cell modification technology platform is established, a monoclonal cell strain with high anti-apoptosis ability and high disulfide bond catalytic efficiency and protein expression quality is obtained, and a theoretical and technical support is provided for modification of an efficient antibody cell strain which meet the requirements of industrial production.

The present invention provides methods for tumor treatment by administering an inhibitor of quiescin sulfhydryl oxidase 1 (QSOX1), compositions comprising such inhibitors, and methods for identifying such inhibitors.

The invention provides a bioenzyme-containing diesel oil booster composition, comprising a cetane number improver and a bioenzyme at a weight ratio of 1:0.01-1; the bioenzyme is sulfonase, alcohol oxidase, aldehyde Oxidase, amine oxidase, sulfhydryl oxidase, methylmercaptan oxidase, menaquinone oxidase, peroxidase, ferrochelatase, decarboxylase, aldolase, oxoacid lyase, demethylation Mixture of enzymes, alcohol, acid, ester dehydratase, desulfhydrylase, desulfomethylase, cobalt chelatase, magnesium chelatase. A bio-enzyme-containing diesel oil booster composition of the present invention can realize high-speed catalytic performance in a mild environment by utilizing the characteristics of a bio-enzyme, and is used in combination with the existing cetane number improver, The catalytic combustion efficiency of the cetane number improver in diesel is significantly improved, and the combustion performance of diesel is further improved.

The invention discloses an application of rice resting sulfydryl oxidase in improvement of flour processing quality, and belongs to the field of genetic engineering and cereal science. Through a prokaryotic expression system, the recombinant rice resting sulfydryl oxidase with sulfydryl oxidation activity is prepared, and the recombinant rice resting sulfydryl oxidase has the characteristics of high expression quantity, simplicity and convenience in purification, easiness in amplification, suitability for industrial application and the like. The method for improving the flour processing quality by applying the recombinant rice resting sulfydryl oxidase is provided for the first time, the stabilization time of the flour can be remarkably prolonged, the flour property of the flour can be effectively enhanced and the baking quality of bread can be improved by directly adding the recombinant rice resting sulfydryl oxidase into the flour, and the improvement effect is superior to that of a chemical gluten strengthening agent potassium bromate, the enzyme preparation can be developed into a novel biological flour improving enzyme preparation for replacing potassium bromate, is used for being compounded with other biological improvers, and promotes the development of the flour product processing industry.

The invention relates to the technical field of natural colored silk processing, in particular to a complex enzyme for degumming and fixation of natural colored silk and an application method of the complex enzyme. The complex enzyme comprises a bio-enzyme A and a bio-enzyme B; the bio-enzyme A comprises, by weight, 1-10% of papain, 1-10% of bromelain, 0.5-5% of neutral protease, and 0.1-3% of lipase; the bio-enzyme B comprises, by weight, 1-10% of sulfhydryl oxidase and 0.3-5% of protein disulfide isomerase. With the complex enzyme and the application thereof, the biggest obstacle in promotion and application of colored cocoons in the later period is omitted, the pure-natural style of colored silk is guaranteed, the dyeing and finishing process is omitted, and the high-quality environmental protection concept is met.

The invention relates to a method for diagnosing the liver disease by utilizing the property of the serum capable of oxidizing sulfhydryls. The property of the serum capable of oxidizing the sulfhydryls is commonly contributed by resting sulfhydryl oxidase and other macromolecules or micromolecules in the serum. The specific activity of the resting sulfhydryl oxidase in the serum can be detected by a fluorescence method adopting Amplex UltraRed, horseradish peroxidase, dithiothreitol and tween as main reagents. The total activity of the oxidized sulfhydryls in the serum can be detected by a colorimetric method adopting dithiothreitol, dithiobisnitrobenzoic acid and guanidine hydrochloride as main reagents. The method and the kit show that the specific activity of the resting sulfhydryl oxidase in the serum and the total activity of the oxidized sulfhydryls in the serum can be used for diagnosing the liver disease, wherein the diagnosis effect of the total activity of the oxidized sulfhydryls in the serum is better. The invention also relates to a kit for diagnosing the liver disease. The kit adopts phosphate buffer solution, the Amplex UltraRed, the horseradish peroxidase, the dithiothreitol and the tween as the main reagents or adopts the guanidine hydrochloride, the dithiobisnitrobenzoic acid and the dithiothreitol as the main reagents.

The invention discloses a soluble expression system and application thereof in soluble expression of protein. The inventor screens protein disulfide bond isomerase from different sources, and constructs the protein disulfide bond isomerase and sulfydryl oxidase under the control of the same promoter. The expression of the two proteins is optimized through a strategy of adding a nitrogen terminal tag, and a soluble expression system is constructed. In escherichia coli, after co-expression of the system and target protein recombinant porcine carboxypeptidase B, soluble expression of the porcine carboxypeptidase B can be realized, and formation of inclusion bodies is greatly reduced. The soluble expression system can also be used for soluble expression of other target proteins, such as trypsinogen, and has universality of promoting soluble expression of the target proteins. The method has application prospects in the fields of recombinant protein production, new enzyme original screening and the like.

The invention discloses an application of wheat resting sulfydryl oxidase in improving the stability of emulsion type special medical foods, and belongs to the field of genetic engineering and dairy science. The recombinant wheat resting sulfydryl oxidase with sulfydryl oxidation activity is prepared through a prokaryotic expression system, and has the characteristics of being high in expression quantity, simple and convenient to purify, easy to amplify, suitable for industrial application and the like. The invention provides a method for improving the stability of the emulsion type special medical foods by applying the recombinant wheat resting sulfydryl oxidase for the first time, and the recombinant wheat resting sulfydryl oxidase is directly added into the emulsion type special medicalfoods, so that the particle size and the TSI index of emulsion particles can be remarkably reduced, and the storage stability of the emulsion is improved. Therefore, the recombinant wheat resting sulfydryl oxidase can be developed into a biological enzyme preparation for improving the stability of emulsion type special medical foods, and promote the development of the special medical food processing industry in China.