The biosynthesis method of n-methylpyrroline

A technology of methylpyrroline and microorganisms, which is applied in the fields of biocatalysis and metabolic engineering, and can solve problems such as large environmental pollution
CN111100886BActive Publication Date: 2021-07-20CAS CENT FOR EXCELLENCE IN MOLECULAR PLANT SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
CAS CENT FOR EXCELLENCE IN MOLECULAR PLANT SCI
Publication Date
2021-07-20

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Abstract

The invention provides a method for preparing N-methylpyrroline through the combined catalysis of three enzymes, comprising the following steps: using L-ornithine as a substrate, using ornithine decarboxylase EcODC, putrescine-N-methyl It is obtained through joint catalysis of transferase AtPMT and amine oxidase AaDAO3. The above method can also be realized by fermentation of genetically engineered bacteria expressing these three enzymes. The method of the invention is environment-friendly, has warm reaction conditions, and has industrialized development prospects.
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Description

technical field

[0001] The invention belongs to the technical field of biocatalysis and metabolic engineering, and in particular relates to a biosynthesis method of N-methylpyrroline. Background technique

[0002] The compound N-methylpyrrolinium shown in formula I (N-methylpyrrolinium), mainly exists in the form of salt, and molecular formula is C 5 h 10 N + , forming equilibrium with N-methylaminobutyraldehyde in a certain solution environment) is a common key intermediate in the biosynthetic pathway of nicotine from plant sources and plant natural product drugs tropium alkaloids. The biosynthetic route of N-methylpyrroline starting from L-ornithine is reported ((Chase et al.2003; 2004; et al.2005; Ruetsch et al.2001.), see appendix figure 1 , including three steps: L-ornithine is catalyzed by ornithine decarboxylase to generate putrescine, putrescine is catalyzed by N-methyltransferase to generate N-methylated putrescine, N-methylated putrescine It is further cata...

Claims

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