30 results about "Ornithine-Oxo-Acid Transaminase" patented technology

The invention relates to a method for synthesizing a natural product (+ / -)-Pestaloxazine A from ornithine. The method comprises the steps of carrying out series reaction such as condensation, oxidation and cyclization on the protected ornithine to obtain a symmetric dioxazine alkane spiropiperazine compound A; and then carrying out condensation reaction on the symmetric dioxazine alkane spiropiperazine compound A and a side chain compound B to prepare the natural product (+ / -)-pestaloxazine A.

The invention provides a chemical-enzyme method for preparing D-basic amino acid hydrochloride. The method comprises the following steps of: derivatizing DL-basic amino acid serving as a raw material to obtain DL-N-diphenyl acetyl basic amino acid by utilizing an acylating agent, hydrolyzing the DL-N-diphenyl acetyl basic amino acid by using immobilized penicillin acylase as a biocatalyst through non-stereo selectivity and enantioselectivity to obtain L-basic amino acid, phenylacetic acid and D-alpha-N-phenylacetyl basic amino acid; and performing acidolysis on the D-alpha-N-phenylacetyl basic amino acid to obtain phenylacetic acid and D-basic amino acid dihydrochloride, and performing treatment of epoxypropane to obtain the D-basic amino acid hydrochloride. The D-lysine hydrochloride and D-ornithine hydrochloride which are prepared by the method have the yield of more than 40 percent, and ee is more than 99 percent. The method has the characteristics of high yield and optical purity, simple process and the like, and is suitable for the large-scale production of the D-basic amino acid hydrochloride.

The present invention relates to the use of a first amino acid selected from the group consisting of glycine, alanine, cysteine, histidine, leucine, methionine, phenylalanine, serine, tryptophan and tyrosine or a mixture of two or more thereof; a second amino acid selected from the group consisting of aspartic acid, cystine, glutamic acid, glutamine, isoleucine, lysine, aspartic acid, ornithine, threonine, valine, proline and hydroxyproline or a mixture of two or more thereof and one or more furanones for increasing the palatability of a foodstuff to a companion animal. The invention also relates to a pet foodstuff or supplement comprising a first amino acid selected from the group consisting of glycine, alanine, cysteine, histidine, leucine, methionine, phenylalanine, serine, tryptophan and tyrosine or a mixture of two or more thereof; a second amino acid selected from the group consisting of aspartic acid, cystine, glutamic acid, glutamine, isoleucine, lysine, aspartic acid, ornithine, threonine, valine, proline and hydroxyproline or a mixture of two or more thereof and one or more furanones, and also to a method of increasing the palatability of a foodstuff to a companion animal.

Disclosed are enantiomerically pure cyclopentane-based compounds that are prepared by a multiple-step synthesis process. The disclosed compounds have been designed to inhibit gamma-aminobutyric acid-amino transferase (GABA-AT) activity and ornithine aminotransferase (OAT) activity. Some of the enantiomerically pure compounds inhibit OAT activity more potently than the racemic compound. The disclosed compounds may be used to selectively inhibit OAT activity, for example, to treat hepatocellular carcinoma and / or used to selected inhibit GABA-AT activity, for example, to treat neurological diseases and disorders.

The invention discloses a method for producing L-ornithine hydrochloride by TOP10 / PBAD / Thio-TOPO-Arg-Bsub colibacillus genetic engineering bacteria. The strain can express and generate excess bacillus subtilis arginase, and efficiently converts L-arginine into L-ornithine.

The present invention relates to a novel flavour composition for modulating the flavour of a nutritional product, comprising a mixture of a single amino acid and wet alkane polyol, wherein the single amino acid is selected from the group consisting of ornithine, lysine, glycine, alanine, monosodium glutamate, histidine, threonine, phenylalanine, tyrosine, serine, methionine, arginine, glutamic acid and cysteine. The invention also concerning the use of such a flavour composition for modulating the flavor and / or flavour shelf-life of a nutritional product, the process for preparing such a flavour composition and a nutritional product comprising such a flavour composition.

Disclosed herein are methods of treating and preventing muscle loss using ornithine in combination with at least one of phenyl acetate and phenylbutyrate.

An external standard solution for use in the analysis of amino acid in plasma, containing,(1) at least one amino acid selected from the following components A, at a concentration of 0.0007 M to 0.49 M, and (2) (i) at least one amino acid selected from the following components B, at a concentration of 0.2 to 0.9 times of the lowest-concentration amino acid among the amino acids selected from components A, (ii) at least one amino acid selected from the following components C, at a concentration of 0.1 to 0.4 times of the lowest-concentration amino acid among amino acids selected from the components A, or (iii) at least one amino acid selected from the following components D, at a concentration of 0.05 to 0.2 times of the lowest-concentration amino acid among amino acids selected from the components A;[Components A] valine, glycine, alanine and glutamine[Components B] serine, proline, threonine, taurine, leucine, isoleucine, lysine, histidine, phenylalanine and tyrosine[Components C] asparagine, ornithine, arginine and tryptophan[Components D] glutamic acid, methionine, citrulline and cystine.

The invention discloses a method for evaluating the temperature and humidity state of the environment where nursery pig individuals grow. According to the method, biochemical indexes and metabolite concentration in nursery pig blood are quantitatively determined, and the indexes are used for evaluating the temperature and humidity state of the environment where nursery pigs grow. Biochemical indexes and metabolite indexes in blood are as follows: biochemical indexes and metabolite indexes in blood; albumin and glutamic oxalacetic transaminase are added; glutamic-pyruvic transaminase, blood urea nitrogen, creatinine, glucose, triglyceride, total cholesterol, lactic acid, creatine kinase, immunoglobulin M, 3-methyl-L-histidine, ortho-phosphoethanolamine, L-carnosine, L-arginine, ethanolamine, L-glycine, L-aspartic acid, L-sarcosine, beta-alanine, L-ornithine, L-cystine, L-tyrosine, hydroxyproline and alpha-serine.

The invention relates to the technical field of medicines, in particular to a targeting inducible nitric oxide synthase (iNOS) triptolide prodrug, and a preparation method and application thereof. R1of the triptolide targeting prodrug is L-arginine, L-arginine methyl ester, L-Nomega-NO-arginine, L-N omega-NO-arginine methyl ester, L-proline, L-citrulline, L-thiocitrulline or L-ornithine. The highexpression characteristic of the inducible nitric oxide synthase (iNOS) at an inflammatory site is utilized, and the triptolide is semi-synthesized by an iNOS specific substrate (group) to prepare the prodrug, the toxicity of the triptolide is reduced and the bioavailability are improved through the targeting property of the prodrug, the anti-inflammatory effect is significantly better than thatof the triptolide, and the triptolide can be effectively developed and utilized.

The invention relates to a zymologic method for circularly testing the activity of dimethyl arginine dimethylamine hydrolytic enzyme, and a diagnostic reagent thereof. In a designed product detection method, hydrolysis of asymmetric dimethyl arginine is catalyzed by dimethyl arginine dimethylamine hydrolytic enzyme in a tissue sample to generate citrulline; in a novel design method, citrulline is made to continuously react with an enzyme circular reaction system consisting of citrulline hydrolytic enzyme (EC 3.5.1.20), ornithine carbamoyltransferase (EC 2.1.3.3) and a substrate of the ornithine carbamoyltransferase, so that the reaction sensitivity is greatly enhanced, and the yield of the citrulline is proportional to the activity of the dimethyl arginine dimethylamine hydrolytic enzyme in the sample; and the aim of testing the activity of the dimethyl arginine dimethylamine hydrolytic enzyme in the sample can be fulfilled by testing the generating rate of ammonia in the circular enzyme reaction system. A reagent can be a dry powder reagent, a liquid reagent, a single-dose reagent, a double-dose agent or a multi-dose agent.

The invention discloses a preparation method of enzymolysis ovalbumin, and belongs to the technical field of enzymolysis ovalbumin production. The enzymolysis ovalbumin is prepared from the following raw materials in parts by weight: 0.21 to 0.51 part of ornithine, 1.50 to 6.00 parts of lysine, 0.80 to 1.80 parts of histidine, 2.25 to 5.50 parts of arginine, 0.80 to 3.80 parts of threonine, 1.50 to 6.00 parts of serine, 1.41 to 8.24 parts of glutamic acid, 1.00 to 2.58 parts of proline, 0.30 to 2.98 parts of glycine, 1.86 to 5.17 parts of alanine, and the balance of water. The immobilized enzyme can be recycled and can be repeatedly used, so that the investment of the preparation cost is reduced, the immobilized enzyme can be easily and conveniently recycled, time and labor are saved, and the production efficiency of the enzymolysis ovalbumin is guaranteed.

Disclosed are enantiomerically pure cyclopentane-based compounds that are prepared by a multiple-step synthesis process. The disclosed compounds have been designed to inhibit gamma-aminobutyric acid-amino transferase (GABA-AT) activity and ornithine aminotransferase (OAT) activity. Some of the enantiomerically pure compounds inhibit OAT activity more potently than the racemic compound. The disclosed compounds may be used to selectively inhibit OAT activity, for example, to treat hepatocellular carcinoma and / or used to selected inhibit GABA-AT activity, for example, to treat neurological diseases and disorders.

The invention belongs to the technical field of microorganism fermentation and food nutrition, and particularly relates to a compound amino acid nutrition bag and a preparation method thereof. The compound amino acid nutrition bag is prepared from L-arginine, L-threonine, L-ornithine, L-lysine, L-proline, L-glutamic acid, L-valine, L-phenylalanine, L-leucine, organic protein and reducing sugar. The compound amino acid nutrition bag is rich in nutritional ingredient, green, healthy, fragrant and sweet in taste, diverse in type and capable of being widely applied to feed. The preparation method comprises the processes of L-arginine seed enlarge culturing, fermental culturing, micro-filtering for bacterium removal, concentration cream forming or concentration drying, and the preparation method is simple.

The invention discloses a composition for soothing liver-gallbladder, detoxifying and protecting liver and a preparation method thereof. The composition is prepared from the following raw materials in parts by weight: 12-15 parts of liver extract, 20-22 parts of silybum marianum, 9-11 parts of ornithine and 3-5 parts of turmeric. The composition can significantly improve abnormality of indexes of glutamic oxalacetic transaminase, glutamic-pyruvic transaminase, total protein, albumin, globulin, total bilirubin, direct bilirubin, total bile acid and alkaline phosphatase, repair liver injury, significantly enhance the hangover alleviating ability and improve bad symptoms after drinking or after drunkenness, and the composition is easy to industrially produce and popularize and use.

The present invention relates to the technical field of inspection and measurement. Disclosed is an enzymology detection method for detecting concentration of citrulline and asymmetric dimethylarginine (ADMA) in a reaction system by using an ATP detection method. The basic principle of the reaction is mainly as follows: ADMA is hydrolyzed by using dimethylarginine dimethylaminohydrolase to obtain citrulline; when ADP and Mg2+ exist, the citrulline is coupling-catalyzed by using ornithine carbamoyltransferase and carbamoyl phosphate kinase to finally obtain ornithine, carbon dioxide, ammonia gas, and ATP, wherein the released ATP can be detected by using a reagent. The present invention has the advantages of flexible and quick reaction, high accuracy, and low cost; ADMA or citrulline with the concentration of 0.1μmol / L at lowest can be detected, and the detection result approaches the concentration of ADMA in the normal serum and is far less than the concentration of citrulline in the normal serum, thereby providing a new applicable method for clinical ADMA detection.

The invention provides a method for preparing N-methylpyrroline through the combined catalysis of three enzymes, comprising the following steps: using L-ornithine as a substrate, using ornithine decarboxylase EcODC, putrescine-N-methyl It is obtained through joint catalysis of transferase AtPMT and amine oxidase AaDAO3. The above method can also be realized by fermentation of genetically engineered bacteria expressing these three enzymes. The method of the invention is environment-friendly, has warm reaction conditions, and has industrialized development prospects.

The invention relates to the field of pharmaceutical preparations, in particular to an ornithine-aspartate injection and a preparation method thereof. If impurities such as amide, arginine, and dimeric ornithine exceed the standard, the following prescription is adopted: including pre-purified aspartic acid ornithine, metal ion complexing agent, pH buffer pair and water for injection, in which the pH buffer pair In order to maintain the stable pH of the injection, the metal ion complexing agent avoids the decomposition and catalysis of the main drug by the metal ion; the preparation process of the present invention includes: two parts: pre-purification of aspartic acid ornithine and preparation of the medicinal solution, wherein Pre-purification adopts the method of recrystallization. During the preparation of the medicinal solution, two sterilization methods are adopted: 121°C high-pressure steam sterilization for 15 minutes and 0.22 μm microporous membrane. The injection of the present invention has stable physical and chemical properties and low impurity content , can be stored for a long time.

The present invention relates to a method for manipulating the high mannose glycoform content of recombinant glycoproteins by modulating ornithine metabolism during cell culture.