30 results about "Ornithine-Oxo-Acid Transaminase" patented technology

The invention discloses a method for producing L-ornithine hydrochloride by TOP10 / PBAD / Thio-TOPO-Arg-Bsub colibacillus genetic engineering bacteria. The strain can express and generate excess bacillus subtilis arginase, and efficiently converts L-arginine into L-ornithine.

The present invention relates to a novel flavour composition for modulating the flavour of a nutritional product, comprising a mixture of a single amino acid and wet alkane polyol, wherein the single amino acid is selected from the group consisting of ornithine, lysine, glycine, alanine, monosodium glutamate, histidine, threonine, phenylalanine, tyrosine, serine, methionine, arginine, glutamic acid and cysteine. The invention also concerning the use of such a flavour composition for modulating the flavor and / or flavour shelf-life of a nutritional product, the process for preparing such a flavour composition and a nutritional product comprising such a flavour composition.

Disclosed herein are methods of treating and preventing muscle loss using ornithine in combination with at least one of phenyl acetate and phenylbutyrate.

The invention relates to a zymologic method for circularly testing the activity of dimethyl arginine dimethylamine hydrolytic enzyme, and a diagnostic reagent thereof. In a designed product detection method, hydrolysis of asymmetric dimethyl arginine is catalyzed by dimethyl arginine dimethylamine hydrolytic enzyme in a tissue sample to generate citrulline; in a novel design method, citrulline is made to continuously react with an enzyme circular reaction system consisting of citrulline hydrolytic enzyme (EC 3.5.1.20), ornithine carbamoyltransferase (EC 2.1.3.3) and a substrate of the ornithine carbamoyltransferase, so that the reaction sensitivity is greatly enhanced, and the yield of the citrulline is proportional to the activity of the dimethyl arginine dimethylamine hydrolytic enzyme in the sample; and the aim of testing the activity of the dimethyl arginine dimethylamine hydrolytic enzyme in the sample can be fulfilled by testing the generating rate of ammonia in the circular enzyme reaction system. A reagent can be a dry powder reagent, a liquid reagent, a single-dose reagent, a double-dose agent or a multi-dose agent.

The invention belongs to the technical field of microorganism fermentation and food nutrition, and particularly relates to a compound amino acid nutrition bag and a preparation method thereof. The compound amino acid nutrition bag is prepared from L-arginine, L-threonine, L-ornithine, L-lysine, L-proline, L-glutamic acid, L-valine, L-phenylalanine, L-leucine, organic protein and reducing sugar. The compound amino acid nutrition bag is rich in nutritional ingredient, green, healthy, fragrant and sweet in taste, diverse in type and capable of being widely applied to feed. The preparation method comprises the processes of L-arginine seed enlarge culturing, fermental culturing, micro-filtering for bacterium removal, concentration cream forming or concentration drying, and the preparation method is simple.

The present invention relates to the technical field of inspection and measurement. Disclosed is an enzymology detection method for detecting concentration of citrulline and asymmetric dimethylarginine (ADMA) in a reaction system by using an ATP detection method. The basic principle of the reaction is mainly as follows: ADMA is hydrolyzed by using dimethylarginine dimethylaminohydrolase to obtain citrulline; when ADP and Mg2+ exist, the citrulline is coupling-catalyzed by using ornithine carbamoyltransferase and carbamoyl phosphate kinase to finally obtain ornithine, carbon dioxide, ammonia gas, and ATP, wherein the released ATP can be detected by using a reagent. The present invention has the advantages of flexible and quick reaction, high accuracy, and low cost; ADMA or citrulline with the concentration of 0.1μmol / L at lowest can be detected, and the detection result approaches the concentration of ADMA in the normal serum and is far less than the concentration of citrulline in the normal serum, thereby providing a new applicable method for clinical ADMA detection.

The invention relates to the field of pharmaceutical preparations, in particular to an ornithine-aspartate injection and a preparation method thereof. If impurities such as amide, arginine, and dimeric ornithine exceed the standard, the following prescription is adopted: including pre-purified aspartic acid ornithine, metal ion complexing agent, pH buffer pair and water for injection, in which the pH buffer pair In order to maintain the stable pH of the injection, the metal ion complexing agent avoids the decomposition and catalysis of the main drug by the metal ion; the preparation process of the present invention includes: two parts: pre-purification of aspartic acid ornithine and preparation of the medicinal solution, wherein Pre-purification adopts the method of recrystallization. During the preparation of the medicinal solution, two sterilization methods are adopted: 121°C high-pressure steam sterilization for 15 minutes and 0.22 μm microporous membrane. The injection of the present invention has stable physical and chemical properties and low impurity content , can be stored for a long time.

The present invention relates to a method for manipulating the high mannose glycoform content of recombinant glycoproteins by modulating ornithine metabolism during cell culture.