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Bifidobacteria viable bacteria preparation and special-purpose protective agent thereof

A technology of bifidobacteria and live bacteria preparations, applied in the direction of bacteria, etc., can solve the problems of unresolved storage at room temperature and easy inactivation, and achieve the effect of solving the difficulty of storage at room temperature, easy operation, and high application value

Active Publication Date: 2007-08-15
LIVZON PHARM GRP INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Strains are easily inactivated during production, transportation and storage
In order to keep the number of viable bacteria in the product at a certain level, researchers at home and abroad have done a lot of research on bacterial genetic modification, microencapsulation technology, and bacterial cell embedding technology. However, from the current point of view, the product is often used It is required to be stored at low temperature, and most products containing bifidobacteria have not solved the problem of room temperature storage

Method used

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  • Bifidobacteria viable bacteria preparation and special-purpose protective agent thereof

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Experimental program
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Effect test

Embodiment 1

[0021] Embodiment 1, the preparation of bifidobacterium live bacteria preparation and its storage time test

[0022] One, the preparation of Bifidobacterium live bacteria preparation

[0023] 1. Activation of Bifidobacteria

[0024] The strain Bifidobacterium adolescentis DM8504 (purchased from Dalian Medical College) was inoculated on the modified BS agar slant medium. Then put it into an anaerobic incubator, and cultivate it anaerobically at a temperature of 37±0.5° C. for 48-72 hr.

[0025] Components of the improved BS medium: tomato juice 200ml, soybean peptone 15g, yeast extract 6g, glucose 10g, Tween 80 1g, tryptone 10g, beef extract 0.3g, soluble starch 0.5g, beef liver extract 5ml, compound salt Solution 4.0ml, L-cysteine ​​hydrochloride 0.5g, agar 15g (used when preparing solid medium). Wherein the composition of compound salt solution is: K 2 HPO 4 1.0g, CaCl 2 0.2g, MgSO 4 ·7H 2 O 0.48g, KH 2 PO 4 1.0g, NaHCO 3 10.0g, NaCl 2.0g.

[0026]The preparat...

Embodiment 2

[0071] Embodiment 2, the preparation of bifidobacterium live bacteria preparation and its storage time test

[0072] One, the preparation of Bifidobacterium live bacteria preparation

[0073] 1. Activation of Bifidobacteria

[0074] The strain Bifidobacterium infantis ATCC 15697 (purchased from the American ATCC Culture Collection Center) was inoculated on the slant medium of the modified TPY medium. Then put it into an anaerobic incubator, and cultivate it anaerobically at a temperature of 37±0.5° C. for 48-72 hr.

[0075] The above-mentioned improved TPY medium is made of the following components: tryptone 12g / L, peptone 3g / L, soybean peptone 5g / L, glucose 5g / L, maltooligosaccharide 5g / L, yeast extract powder 5g / L L, Tween-801.0g / L, L-cysteine ​​hydrochloride 0.5g / L, beef liver extract 200mL / L, tomato juice 100mL / L, mixed salt solution 5mL / L, distilled water 675mL / L, adjusted pH to 7.0-7.2, 121°C, sterilize for 15-20 minutes.

[0076] Mixed salt solution: K 2 HPO 4 20...

Embodiment 3

[0112] Embodiment 3, the preparation of bifidobacterium live bacteria preparation and its storage time test

[0113] One, the preparation of Bifidobacterium live bacteria preparation

[0114] 1. Activation of Bifidobacteria

[0115] The strain Bifidobacterium adolescentis CGMCC 1.2190 (purchased from China General Microorganism Culture Collection and Management Center) was inoculated on the modified BS agar slant medium. Then put it into an anaerobic incubator, and cultivate it anaerobically at a temperature of 37±0.5° C. for 48-72 hr.

[0116] Components of the improved BS medium: tomato juice 200ml, soybean peptone 15g, yeast extract 6g, glucose 10g, Tween 80 1g, tryptone 10g, beef extract 0.3g, soluble starch 0.5g, beef liver extract 5ml, compound salt Solution 4.0ml, L-cysteine ​​hydrochloride 0.5g, agar 15g (used when preparing solid medium). Wherein the composition of compound salt solution is: K 2 HPO 4 1.0g, CaCl 2 0.2g, MgSO 4 ·7H 2 O 0.48g, KH 2 PO 4 1.0...

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Abstract

The invention discloses a preparing method of bifidobacteria bacterial active agent and single-purpose protecting agent, which is characterized by the following: allocating 0.01-5.0 wt glycerin, 0.1-6.0 wt polyvinyl pyrrolidon, 0.1-5.0 carbowax, 1.0-10.0 wt skimmed milk powder, 0.5-15.0 wt mycose, 0.1-5.0 wt glutavene, 0.1-5.0 wt L-aspartic acid, 0.1-5.0 wt vitamin C sodium and 0.1-5.0 wt ornithine hydrochlorate. This invention solves the problem of preservation difficulty at normal temperature with conventional method.

Description

technical field [0001] The invention relates to a live bifidobacterium preparation and a special protective agent thereof. Background technique [0002] Bifidobacterium (Lactobacillus bifidus) is a class of Gram-positive anaerobic bacteria, which is one of the most abundant and common normal flora in the intestinal tract of healthy people. In 1899, it was first isolated from the feces of healthy breastfed infants by Dr. Tissier of the Pasteur Institute in France. After more than 30 years of research, researchers from Japan and other countries, including European countries, gradually discovered the beneficial effects of bifidobacteria on the human body, and it has become a function of measuring the health of the body and evaluating the improvement of gastrointestinal flora. One of the symbols of sexual food. Bifidobacterium is the dominant bacterium in the gastrointestinal tract of healthy adults and long-lived elderly people. It changes dynamically with age and is closely ...

Claims

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Application Information

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IPC IPC(8): C12N1/20
Inventor 苏日佳胡振湘徐乐意王云华王燕清
Owner LIVZON PHARM GRP INC
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