Protective agent of live bacillus subtilis preparation

A technology of Bacillus subtilis and live bacterial preparations, which is applied in the direction of bacterial medical raw materials, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc., which can solve the problems of high freezing costs, inconvenient transportation, and frozen storage. Strong center dependence and other problems, to achieve high application value, solve the difficulty of storage at room temperature, and easy to operate

Inactive Publication Date: 2011-04-06
TIANJIN RINGPU BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the preservation period of liquid live bacteria can be significantly extended by the method of cryopreservation, the cost of maintaining freezing is high, the transportation is inconvenient, and the dependence on the cryopreservation center is too strong

Method used

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  • Protective agent of live bacillus subtilis preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Embodiment 1: the preparation of bacillus subtilis live bacteria preparation

[0012] Activation of Bacillus subtilis strains The strains were inoculated on beef extract peptone solid medium. Then put it into an incubator and cultivate it at 37°C for 16 hours;

[0013] Fermentation culture of Bacillus subtilis The above-mentioned activated cultured colonies were eluted, added to beef extract peptone liquid medium by aseptic method, and cultivated for 16 hours at 37°C and 200 rpm.

[0014] Preparation of live Bacillus subtilis preparations The above-mentioned fermented fermentation liquid was introduced into a centrifuge through a pipeline, and centrifuged with a large-capacity centrifuge at 6000 rpm for 15 minutes at low temperature (4° C.). Collect the sludge-like bacteria in the centrifuge tube, and dry under reduced pressure at 40°C to obtain 10 g of dry bacteria powder of Bacillus subtilis, and the number of viable bacteria was checked to be 2.2×10 16 cfu / g.

[0...

Embodiment 2

[0017] Embodiment 2: Stability detection of Bacillus subtilis live bacteria preparation

[0018] Divide the live Bacillus subtilis preparation prepared according to the method of Example 1 into 2 parts, and place them in room temperature and 37°C sample storage box. The samples placed at room temperature are sampled every 3 months to detect the number of viable bacteria and the viable index. Placed samples are taken once a month to detect the number of live bacteria.

[0019] Viable bacteria count detection method:

[0020] 1. Fully shake the liquid sample, draw 0.5ml of the liquid sample into the test tube, add 4.5ml of diluent, and shake fully to form a 1:10 test solution;

[0021] 2. Dilution: draw 0.5ml of the test solution, add 4.5ml of the diluent to another test tube, mix well to make a 1:100 test solution, repeat the above operation, and perform 10-fold serial dilution until the required dilution;

[0022] 3. Dropping sample: Take 0.2ml of the bacterial solution of ...

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PUM

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Abstract

The invention discloses a protective agent of a live bacillus subtilis preparation, comprising the following components (W/V): 3-4.5% of lactose, 0.5-1% of sodium carboxymethylcellulose, 0.5-1% of potassium sorbate, 0.1-0.5% of vitamin E, 0.1-0.5% of vitamin C, and 0.1-0.2% of water quality lemon. The protective agent of the invention solves the problem that the bacillus subtilis preparation produced by the conventional method is difficult to be stored, and has the advantages of simple and convenient method, easy operation, suitability for the batch preparation of the bacillus subtilis preparation, and high application value.

Description

technical field [0001] The invention relates to the field of biological products, and in particular provides a protective agent for live bacillus subtilis preparations. Background technique [0002] Bacillus subtilis is an obligate aerobic bacterium with a strong ability to capture oxygen, but it cannot grow under anaerobic conditions. In poultry intestines, although Bacillus subtilis can be found, the number is very small, even if there is some proliferation, it will be quickly metabolized. Therefore, the research and development of Bacillus subtilis products and supplementing the quantity of Bacillus subtilis colonized in the intestine by exogenous means are of great significance for adjusting the balance of intestinal microecology, improving immunity and promoting health. [0003] In recent years, the industry of Bacillus subtilis products at home and abroad has developed rapidly and has become one of the fastest growing industries in the world, occupying an increasingly...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/38A61K47/26A61K47/22A61K47/12A61K47/46A61K35/74A61P1/00A23K1/16A23K10/18A23K20/00A23K20/10A61K35/742
Inventor 王小娥
Owner TIANJIN RINGPU BIO TECH
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