Enzymological detection method

A detection method and enzymological technology, applied in the field of medical testing, can solve the problems of inability to apply clinical large-flow automatic analysis, high price, complicated operation, etc., and achieve high scientific value and economic value, simple operation, and sensitive detection effect
CN103207173BInactive Publication Date: 2015-06-17SHAOXING INST OF TECH COLLEGE OF ENG PEKING UNIV BIOENG CENT
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SHAOXING INST OF TECH COLLEGE OF ENG PEKING UNIV BIOENG CENT
Publication Date
2015-06-17
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention relates to the technical field of inspection and measurement. Disclosed is an enzymology detection method for detecting concentration of citrulline and asymmetric dimethylarginine (ADMA) in a reaction system by using an ATP detection method. The basic principle of the reaction is mainly as follows: ADMA is hydrolyzed by using dimethylarginine dimethylaminohydrolase to obtain citrulline; when ADP and Mg2+ exist, the citrulline is coupling-catalyzed by using ornithine carbamoyltransferase and carbamoyl phosphate kinase to finally obtain ornithine, carbon dioxide, ammonia gas, and ATP, wherein the released ATP can be detected by using a reagent. The present invention has the advantages of flexible and quick reaction, high accuracy, and low cost; ADMA or citrulline with the concentration of 0.1μmol / L at lowest can be detected, and the detection result approaches the concentration of ADMA in the normal serum and is far less than the concentration of citrulline in the normal serum, thereby providing a new applicable method for clinical ADMA detection.
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Description

technical field

[0001] The invention relates to the technical field of medical testing, in particular to an enzymatic detection method using ATP chemiluminescence method for detection. Background technique

[0002] Asymmetric dimethylarginine (ADMA) is a methylated protein degradation product, it is a nitric oxide synthase inhibitor, it can competitively inhibit the activity of nitric oxide synthase, and reduce the production of nitric oxide , and can directly induce oxidative stress, as an endogenous relaxation factor, nitric oxide has the function of relaxing blood vessels, and plays an important role in diseases of the cardiovascular system. ADMA widely exists in human tissues, cells, plasma, and urine. It is mainly produced by the methylation of intracellular proteins under the action of arginine methyltransferase, and then hydrolyzed. 80% of ADMA in the human body ADMA is metabolized and inactivated by dimethylarginine dimethylamine hydrolase (DDAH), and only a small p...

Claims

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