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Echinocandin B synthetic medium and application

A technology for synthesizing culture medium and echinocandin, which is applied in the field of culture medium, can solve the problems of poor stability and poor repeatability, and achieve the effects of good repeatability, simple preparation and clear chemical composition

Active Publication Date: 2021-04-13
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The purpose of the present invention is to provide a synthetic medium with high yield of echinocandin B to solve the problems of poor stability and poor repeatability in the fermentation process of echinocandin B in the prior art. The synthetic medium of the present invention is suitable for For the cultivation of Aspergillus nidulans to efficiently produce echinocandin B

Method used

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  • Echinocandin B synthetic medium and application
  • Echinocandin B synthetic medium and application
  • Echinocandin B synthetic medium and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Aspergillus nidulans CCTCC M 2020495

[0027] Aspergillus nidulans (Aspergillus nidulans) CCTCC M 2020495 is obtained by the following steps

[0028] Aspergillus nidulans (Aspergillus nidulans) CCTCC NO: M 2012300 (disclosed in patent application CN2013104776708) was subjected to ARTP mutagenesis treatment, spread on a plate, and cultured for 12-14 days to obtain a mutagenesis treatment mutant strain. The specific method is as follows:

[0029] (1) Aspergillus nidulans (Aspergillus nidulans) CCTCC M 2012300 was inoculated into the activation medium, and cultured at 25° C. in the dark for 12-14 days to obtain activated Aspergillus nidulans. The final concentration of the activation medium consists of: potassium nitrate 3g / L, K 2 HPO 4 ·3H 2 O 1g / L, MgSO 4 ·7H 2 O 0.5g / L, KCl 0.5g / L, FeSO 4 ·7H 2 O 0.01g / L, sucrose 30g / L, agar 20g / L, the solvent is water, and the pH is natural; wash the plate with dark green spores on the surface after 12-14d growth with ...

Embodiment 2-8

[0051] Under the same conditions in Comparative Example 1, medium 1 was replaced by medium 2-medium 8 in Table 1, and the corresponding echinocandin B productions were 568mg / L, 1023mg / L, 1196mg / L, 1612mg / L , 1359mg / L, 1584mg / L, 1877mg / L, the results are shown in Table 2.

Embodiment 9

[0053] Change the culture medium of Comparative Example 1 into medium 5 in table 1, insert the inoculum size of the seed liquid according to the volume concentration 10% in the 5L fermenter that 3L fermented echinocandin B synthetic medium is housed and carry out fermentation culture, ferment After culturing for 240 hours, during the entire fermentation process, the fermentation temperature was controlled at 25°C, the ventilation ratio was 1:1.8, and after the fermentation was completed at 450rpm, the production of echinocandin B reached 1600mg / L.

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Abstract

The invention discloses an echinocandin B synthetic medium and application of the echinocandin B synthetic medium in preparation of echinocandin B. The echinocandin B synthetic medium is prepared from, by mass concentration, 20-100g / L of methyl oleate, 10-60g / L of starch, 10-30g / L of oleic acid, 10-30g / L of histidine, 5-20g / L of glutamic acid, 2-5g / L of L-threonine, 5-8g / L of L-ornithine hydrochloride, 5-15g / L of potassium nitrate, 5-20g / L of K2HPO4.3H2O, 0.1-1g / L of MgSO4.7H2O, 0.1-0.5 g / L of MnSO4.H2O, 0.01-0.1g / L of FeSO4.7H2O, 0.1-0.5g / L of CaCl2, and 0.5-1g / L of CuSO4.5H2O, wherein water serves as a solvent, and pH is 6.5-7.0. The yield of echinocandin B reaches 1950mg / L.

Description

[0001] (1) Technical field [0002] The invention relates to a culture medium, in particular to a synthetic culture medium for producing echinocandin B. [0003] (2) Background technology [0004] In recent years, due to the abuse of antibiotics, the deterioration of the environment, and the aging population, fungal infections have attracted more and more attention. Fungal infections are infectious diseases of humans and animals caused by fungi. Among them, Candida infection accounts for the highest proportion of deep infections in my country, reaching 91%. The fatality rate of invasive candida infection is reported to be 30%, and if left untreated, the mortality rate can be as high as 100%. Therefore, the diagnosis and treatment of antifungal infections, especially the research and development of antifungal drugs has become a hot spot. [0005] Antifungal drugs currently on the market are mainly polyenes, azoles, fluoropyrimidines and echinocandins. Alkenes and azoles gene...

Claims

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Application Information

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IPC IPC(8): C12P21/04C07K7/56C12N1/14C12R1/66
CPCC12P21/02C07K7/56C12N1/14
Inventor 胡忠策郎克鹏牛坤邹树平柳志强郑裕国
Owner ZHEJIANG UNIV OF TECH
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