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Preparation method of modified glassy carbon electrode as glucose sensor and application thereof

A technology of glucose sensor and glassy carbon electrode, which is applied in the field of electrochemical analysis and detection, can solve the problems of low electron transfer efficiency of enzymes, and achieve the effects of high catalytic performance, high detection efficiency and low cost

Inactive Publication Date: 2009-11-18
SHANGHAI UNIV
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Problems solved by technology

However, because the active site of the enzyme is located deep inside the protein, the electron transfer efficiency of the enzyme in the biosensor is very low without an electronic mediator, and the addition of an electronic mediator in the solution can improve the detection sensitivity of the sensor.

Method used

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  • Preparation method of modified glassy carbon electrode as glucose sensor and application thereof
  • Preparation method of modified glassy carbon electrode as glucose sensor and application thereof

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Embodiment 1

[0017] The preparation process and steps in this embodiment are as follows:

[0018] (1) Pretreatment of the glassy carbon electrode: First, the glassy carbon electrode is polished with metallographic sandpaper, and then 1.0 μm, 0.3 μm and 0.05 μm Al 2 o 3 The suspension was polished to a mirror finish on suede, and finally with 1:1 HNO 3 Solution, absolute ethanol and twice distilled water were ultrasonically cleaned and set aside;

[0019] (2) Preparation of glassy carbon electrode modifier: a certain amount of chitosan is dissolved in acetic acid solution to prepare a chitosan solution with a mass solubility of 0.5%; a certain amount of spinel-type nano-nickel ferrite Disperse in the above chitosan solution, and then ultrasonically disperse for 2 hours to form a dispersion containing spinel nano-nickel ferrite; then add a certain amount of glucose oxidase to the above dispersion, and then ultrasonically disperse for 3~ 10 minutes, make disperse evenly, obtain glassy carb...

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Abstract

The invention relates to a preparation method which uses spinel nano nickel ferrite, chitosan and glucose oxidase mixed dispersion liquid for modifying glassy carbon electrode as glucose sensor and application thereof, belonging to the technical field of electrochemical analysis and detection. In the invention, the spinel nano nickel ferrite is added into the chitosan solution with fixed content of glucose oxidase to improve the transmission of electronic, and is used for electrochemical gaging of the glucose. The preparation method of the modified glassy carbon electrode as glucose sensor is as follows: the spinel nano nickel ferrite is dispersed into the chitosan solution with certain concentration; the mixed solution is evenly dispersed by ultrasound; the glucose oxidase with certain amount is added into the mixed solution; the mixed solution is dispersed evenly by ultrasound once again to obtain a modifier of the nickel ferrite, the chitosan and the glucose oxidase. The modifier is dripwise added on the surface of a clean glassy carbon electrode to be dried for 24 hours at the temperature of 4 DEG C, and then an evenly complex film is formed on the surface of the electrode to obtain the modified glassy carbon electrode which also can be used as glucose sensor and applied into rapid electrochemical gaging of the glucose.

Description

technical field [0001] The invention relates to a preparation method and application of a modified glassy carbon electrode used as a glucose sensor modified by a mixed dispersion of spinel-type nano-nickel ferrite, chitosan and glucose oxidase, and belongs to the technical field of electrochemical analysis and detection. Background technique [0002] A biosensor combines the techniques and methods of analytical chemistry and biology, and is a device that uses biological factors or biological principles to detect or measure compounds. It usually consists of a molecular recognition part (sensitive element) and a conversion part (transducer). Electrochemical biosensor is the most studied type in the field of biosensors, and it has been widely studied because of its advantages such as: simple structure, no need for expensive detection equipment; easy operation, the transducer converts the reaction energy into Electrical signal, easy to detect and convert into digital signal and...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N27/49
Inventor 罗立强李秋霞徐彦红丁亚平王霞李丽
Owner SHANGHAI UNIV
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