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Amine oxidase NDAO, preparation method and application

A technology for the separation and purification of amine oxidase, applied in the field of agricultural biology, can solve the problems of high cost, limited binding ability, high risk of biological safety, etc., and achieve the effect of reducing harm

Pending Publication Date: 2021-12-21
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although many physical and chemical detoxification strategies have been developed to eliminate mycotoxin contamination, they cannot meet the production requirements due to high cost, high biosafety risk, and limited binding capacity. Therefore, in order to ensure food safety, the development of biological detoxification methods research is necessary

Method used

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  • Amine oxidase NDAO, preparation method and application
  • Amine oxidase NDAO, preparation method and application
  • Amine oxidase NDAO, preparation method and application

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Embodiment Construction

[0025] The embodiments of the present invention will be described in detail below. It should be noted that the embodiments are illustrative, not restrictive, and cannot limit the protection scope of the present invention.

[0026] The raw materials used in the present invention, unless otherwise specified, are conventional commercially available products; the methods used in the present invention, unless otherwise specified, are conventional methods in the art.

[0027] An amine oxidase NDAO, the amino acid sequence of the amine oxidase NDAO is SEQ ID NO.1.

[0028] The preparation method of the above-mentioned amine oxidase NDAO, the steps are as follows:

[0029] (1) Using molecular biology means to recombine the gene encoding the amine oxidase NDAO into a vector and then transform the host cell to obtain a recombinant strain;

[0030] (2) Activating and culturing the recombinant strain to induce the expression of amine oxidase NDAO;

[0031] (3) Separating and purifying a...

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Abstract

The invention relates to an amine oxidase NDAO. The amino acid sequence of the amine oxidase NDAO is SEQ ID NO. 1. The degradation rate of the amine oxidase NDAO provided by the invention on HFB1 is 100%. Amine oxidase NDAO is excellent in property, can be applied to industries such as agriculture, feeds and foods, and reduces harm of Fumonisins FB1 to animal and human health.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology, in particular to an amine oxidase NDAO, a preparation method and application. Background technique [0002] Fumonisins are a class of water-soluble mycotoxins mainly produced by Fusarium species. Fumonisins have a variety of toxicity, including neurotoxicity, immunotoxicity, tissue and organ toxicity, cytotoxicity, etc., and can also cause harm to humans. At present, fumonisins are polluted all over the world, mainly contaminating corn and other grains, which not only cause economic losses, but also pose a great threat to the health of humans and animals. As the study of mycotoxins has become more and more in-depth, people have become more and more aware of the hazards of toxins. In recent years, with the gradual increase of global food crop production, fungal pollution has become more and more serious, and people urgently need to eliminate the hazards of fumonisins to ensure the safet...

Claims

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Application Information

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IPC IPC(8): C12N9/06C12N15/53C12N15/70C12N1/21A23L5/20C12R1/19
CPCC12N9/0022C12N15/70A23L5/25C12Y104/03006
Inventor 李中媛夏忠强张同存
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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