Lactobacillus plantarum AB-3 having bacteria inhibition activity and application thereof

A technology of Lactobacillus plantarum and antibacterial activity, which is applied to a strain of Lactobacillus plantarum AB-3 with antibacterial activity and its application field, which can solve problems such as lack of

Active Publication Date: 2015-08-05
INNER MONGOLIA AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Therefore, there is a lack of green and pollution-free prevention and control methods for such crop diseases in the prior art, that is, biological control, such as using probiotics to control pests and d...

Method used

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  • Lactobacillus plantarum AB-3 having bacteria inhibition activity and application thereof
  • Lactobacillus plantarum AB-3 having bacteria inhibition activity and application thereof
  • Lactobacillus plantarum AB-3 having bacteria inhibition activity and application thereof

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Experimental program
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Effect test

preparation example Construction

[0079] Preparation of Lactobacillus plantarum fermentation supernatant: Inoculate the preserved Lactobacillus plantarum solution in the cryopreservation tube into 5ml of MRS medium, shake and mix well, culture at a constant temperature of 37°C for 24 hours, activate three generations continuously, and take the third generation fermentation liquid Centrifuge at 4000×g for 10 min, take the supernatant, filter the supernatant through a 0.22 μm micro-membrane, and store it in a -20°C refrigerator for later use.

[0080]Indicator bacterium: Inoculate Phytophthora meloni on PDA slant medium, culture in a 28°C incubator for 4-7 days, observe the colony morphology and optimal culture time, restore the activity of the indicator bacteria to normal, and use PBS buffer ( Containing 0.05% Tween 80) to clean its culture medium, wash into 90mL of PBS damping fluid and make 10 5 cfu / ml of Phytophthora meloni spore suspension.

[0081] The cultivation (or activation) conditions of Fusarium me...

Embodiment 1

[0083] Example 1 Determination of the inhibitory activity of Lactobacillus plantarum AB-3 to Phytophthora melon, Penicillium loudii, Melon wilt and apple anthracnose.

[0084] The test was carried out by the hole-diffusion method, and 2 small holes were evenly punched on each plate with a 7mm hole puncher, and the test number of Lactobacillus plantarum AB-3 was marked, and 100 μL of the MRS fermentation supernatant of the Lactobacillus plantarum was added to each hole , diffused in a 4°C refrigerator for 12h, then cultured at a constant temperature of 28°C for 48h, observed the size of the inhibition zone, and measured the size of the inhibition zone of the Phytophthora melon strain with a vernier caliper. The antibacterial effect of the MRS fermentation supernatant of L.plantarum AB-3 is shown in Table 1.

[0085] Table 1 The size of the inhibition zone of L.plantarum AB-3 against Phytophthora melon (mm)

[0086]

[0087] The MRS fermentation supernatant of L.plantarum AB...

Embodiment 2

[0093] Determination of inhibitory activity of embodiment 2L.plantarum AB-3 to escherichia coli, Salmonella typhimurium and Shigella flexneri

[0094] Dilute the suspensions of indicator bacteria (Escherichia coli, Salmonella typhimurium and Shigella flexneri) to 10 6 Add cfu / mL at 1:100 (V / V) to the sterilized MRS medium cooled to 45°C, shake well, add 20mL / plate quantitatively, cool down to make a plate containing bacteria, and use The 8mm hole puncher evenly punches 6 small holes and marks the bacteria number. Add 100 μL of the corresponding Lactobacillus plantarum MRS or whole milk fermentation supernatant to each well, diffuse in a refrigerator at 4°C for 12 hours, then culture at a constant temperature of 37°C for 48 hours, and observe the effect of L.plantarum AB-3 on Escherichia coli, Salmonella typhimurium and Freund’s The size of the inhibition zone of Shigella, the results are shown in Table 3 and Table 4.

[0095] Table 3 The size of the inhibition zone (mm) agai...

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Abstract

The invention provides a lactobacillus plantarum AB-3 strain having an activity of resisting phytophthora melonis kalsura, of which the accession number is CGMCC No.9655. The lactobacillus plantarum AB-3 has effective inhibition properties on escherichia coli, salmonella typhimurium and shigella flexneri, has effective inhibition properties on the phytophthora melonis kalsura, fusarium oxysporum, penicillium roqueforti and glomerella cingulata and is excellent in thermostability. A fermentation supernate of the strain shows a significantly increased bacteria inhibition rate after treatment at 100 DEG C and is enhanced in bacteria inhibition activity through enzymolysis by a neutral protease. The fermentation supernate of the strain has a storage resistance property and is excellent in bacteria inhibition activity even after normal storage for 20 days.

Description

【Technical field】 [0001] The invention relates to a plant Lactobacillus strain (Lactobacillus plantarum) AB-3 isolated from Sichuan pickles, and the application of the plant Lactobacillus in fermenting food, inhibiting pathogenic bacteria, and in biological pesticides and fruit and vegetable fresh-keeping agents. 【technical background】 [0002] Among all infectious diseases of plants, fungal diseases are the most numerous, accounting for more than 70% of all diseases. Melon Phytophthora is a fungal disease caused by Phytophthora drechsleri Tucker infecting melon plants. Melon Phytophthora is an important disease in melon production, and once it occurs, it is difficult to control. Leaves, stems and fruits can be infected. At present, chemical control is the main measure to control Phytophthora of muskmelon, but long-term use of chemical pesticides will cause pathogenic bacteria to develop resistance, and the disadvantages of chemical residues and environmental pollution are ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23C9/12A23L1/29A23L3/3571A01N63/00A01P3/00A01P1/00A23B7/155C12R1/25A23L33/00
CPCA01N63/00A23B7/155C12R2001/25C12N1/205A23V2400/169
Inventor 陈永福张和平李常坤
Owner INNER MONGOLIA AGRICULTURAL UNIVERSITY
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