Detection card for rapidly and quantitatively detecting penicillin and application thereof

A quantitative detection and penicillin technology, applied in the field of penicillin detection, can solve problems such as low detection sensitivity of carlin, and achieve the effects of improving sensitivity, reducing production cost and shortening detection time.

Pending Publication Date: 2019-12-17
武玉香
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] For this reason, the embodiment of the present invention provides a test card for rapid quantitativ

Method used

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  • Detection card for rapidly and quantitatively detecting penicillin and application thereof
  • Detection card for rapidly and quantitatively detecting penicillin and application thereof
  • Detection card for rapidly and quantitatively detecting penicillin and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] This embodiment is a preparation method of penicillin whole antigen, which preparation method comprises the following steps:

[0037] Weigh 5mg of penicillin G, add 1000μL of distilled water to dissolve, then add 30mg of EDC, mix well; place in a shaker at 25°C, shake at 180rpm for 1h to activate the carboxyl group;

[0038] Weigh 50mg of bovine serum albumin, add 2ml of 0.1M boric acid buffer solution with pH9.0;

[0039] The bovine serum albumin solution was added dropwise to the activated penicillin solution, placed in a shaker at 25° C., and shaken at 180 rpm for 2 hours to obtain the penicillin whole antigen.

[0040] The above-mentioned penicillin whole antigen was identified by ultraviolet scanning; the identification results were as follows: figure 1 shown;

[0041] Depend on figure 1 It can be seen that penicillin G has an absorption peak at 300nm, BSA and other proteins have absorption peaks at 280nm, and penicillin whole antigen has strong absorption peaks...

Embodiment 2

[0043] This embodiment is a method for labeling penicillin-binding protein with quantum dots, the method comprising the following steps:

[0044] Mix 1ml (1000μg) ZnS / CdSe composite quantum dots (produced by Shandong Ludu Biotechnology Co., Ltd., batch number LD028-L) and 1000μg N-hydroxysuccinimide, stir and react at room temperature for 20min, then add 2000μg N, N '-Dicyclohexylcarbodiimide and 2000 μg of penicillin-binding protein (from Shandong Lvdu Biotechnology Co., Ltd., product number LD-Ag089) were reacted at room temperature for 1.5 h, and the supernatant was discarded by centrifugation. Serum protein, 0.05% NaN 3 Dissolve in 0.01M PBS to obtain the quantum dot-labeled penicillin-binding protein.

Embodiment 3

[0046] This embodiment is a method for preparing a binding pad containing a quantum dot-labeled penicillin-binding protein, the preparation method comprising the following steps:

[0047] Select polyester fiber 6613 as the binding pad, soak the binding pad in pH7.4 0.01M PBS buffer containing 0.2% Triton X-100, 0.5% alanine, 0.05% NaN3 for 5min, take it out and dry it at 37°C; The penicillin-binding protein marked with quantum dots of 1 mg / ml (calculated by quantum dot mass) obtained in Example 2 was sprayed on the treated junction with a film-drawing instrument at a concentration of 10 μL / cm, and dried naturally at room temperature to form Conjugate pad containing quantum dot-labeled penicillin-binding protein.

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Abstract

The embodiment of the invention discloses a detection card for rapidly and quantitatively detecting penicillin and an application thereof. The detection card comprises a bottom plate, a sample pad, acombination pad, a base membrane and a water absorption pad, and is characterized in that the combination pad contains quantum dot labeled penicillin binding protein. The detection card disclosed by the invention uses quantum dots to label penicillin binding protein, so that the sensitivity of fluorescence detection can be greatly improved. Besides, the detection card can be used for rapidly, sensitively and quantitatively detecting penicillin, a result can be rapidly obtained by adopting a quantum dot quantitative reading instrument, the detection time is greatly shortened, and convenience isprovided for field detection.

Description

technical field [0001] The embodiments of the present invention relate to the technical field of penicillin detection, in particular to a detection card for rapid quantitative detection of penicillin and its application. Background technique [0002] Penicillin G (penicillin G, benzyl penicillin) is also commonly called penicillin. It is obtained from the culture medium of Penicillium fungus. Penicillin G is an organic acid that can combine with metal ions or organic bases to form salts. Low doses of penicillin do not cause toxicity. When used in large doses, neuropsychiatric symptoms may appear, such as hyperreflexia, sensory disturbance, hallucinations, convulsions, drowsiness, etc., and may also lead to transient mental disorders, which can be recovered by stopping the drug or reducing the dose. In a small number of patients with coagulation disorders, high doses of penicillin can disrupt the coagulation mechanism, leading to bleeding. There is also a Hershey reaction....

Claims

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Application Information

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IPC IPC(8): G01N33/58G01N33/68G01N33/558G01N33/532
CPCG01N33/588G01N33/582G01N33/68G01N33/558G01N33/532
Inventor 武玉香沈志强
Owner 武玉香
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