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Preparation and New Application of Penicillin Binding Protein Antibacterial Agent

A technology that binds protein and penicillin, applied in the field of penicillin-binding protein extract, to achieve the effects of industrial production, ecological environment safety, and good environmental compatibility

Active Publication Date: 2019-03-12
BEIJING GUANGHE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the application of penicillin-binding protein in the preparation of microbial source bacteriostatic agents is rarely reported in the existing literature and actual production. application on

Method used

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  • Preparation and New Application of Penicillin Binding Protein Antibacterial Agent
  • Preparation and New Application of Penicillin Binding Protein Antibacterial Agent
  • Preparation and New Application of Penicillin Binding Protein Antibacterial Agent

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0010] The penicillin-binding protein extract of the present invention is made by the following method: get 10 parts by weight of tryptone, 5 parts by weight of yeast extract (such as produced by Shanghai Jinnan Trading Co., Ltd. or Shandong Yantai Huahai Biological Products Co., Ltd.), and 10 parts by weight of sodium chloride Parts, 1000 parts by volume of distilled water, stirred at room temperature; use a pipette gun to inoculate the strain Staphylococcus aureus into the liquid medium according to the inoculum amount of 1%, put it into a shaker at 36 degrees Celsius, rotate at 180 rpm, and shake for 24 hours Transfer the thalline suspension after shaking culture into a sterilized centrifuge tube, put it into a refrigerated centrifuge with a rotation speed of 8000 rpm for 10 minutes, and take the supernatant; then salt out the crude protein in the supernatant, Then it was purified by anion column chromatography, and compared with a standard product by liquid chromatography t...

experiment example 2

[0012] The bacteriostatic activity determination of the elution peak component of the bacteriostatic protein of the present invention is made by the following method: the measurement is carried out by the plate coating and punching method, and the following work is carried out in an ultra-clean bench. Each component was filtered through a 0.22 μm filter. Take 200uL protein solution of each component and spread evenly on the potato dextrose agar medium plate. Apply 20mmol / L phosphate buffer solution on potato dextrose agar medium as blank control (CK). Potato dextrose agar medium covered with peach brown rot was punched with a hole punch. Use the inoculation needle to pick off the long agar block, and stick the spore side down to the center of the potato dextrose agar medium. Each group was repeated three times. Cultivate at 28°C, observe the antibacterial effect and record the data, that is, measure the antibacterial activity of the elution peak components of antibacterial ...

experiment example 3

[0016] The antibacterial protein crude extract of the present invention has different gradient antibacterial activity assays, which is made by the following method: after obtaining the optimum ammonium sulfate saturation, filter the protein extract under the optimum concentration and then filter the bacteria in the ultra-clean bench according to the gradient Dilute the 4 gradients, take 4 sterilized test tubes, add 5ml crude protein stock solution to the first test tube, then add 4.5ml 20mmol / L phosphate buffer solution to each of the other three test tubes. Take 0.5ml of the stock solution in the second test tube, mix well, which is 10 times dilution; then take 0.5ml of 10 times dilution in the third test tube, mix evenly, that is 100 times dilution; take 0.5ml 100 times dilution In the fourth test tube, mix evenly, which is a 1000-fold dilution. 200uL of each gradient protein solution was evenly spread on the potato dextrose agar medium plate. Apply 20mmol phosphate buffer ...

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Abstract

The present invention discloses a penicillin binding protein extract and novel application thereof. The antibacterial function of the protein has not been reported yet. The penicillin binding protein extract passes through a DEAE-cellulose anion exchange chromatography to obtain a high purity penicillin binding protein; and a flat coating punching method is used for determination of the influence of penicillin binding protein in different concentrations on Monilinia fructicola. Through antibacterial spectrum determination, the protein has the advantages of broad-spectrum antibacterial property, strong antibacterial activity, safety to mammals, no cause of pesticide injury, toxicity residue, safety for people, livestock, and ecological environment. At the same time, the protein is conducive to industrial production, and provides a scientific basis for biological control of agricultural pest.

Description

technical field [0001] The invention relates to a penicillin-binding protein extract and its new application, in particular to a new application of the penicillin-binding protein extract in inhibiting fruit tree or vegetable fungi. Background technique [0002] Crop disease control is an important link in agricultural production. There are many types of pathogens, a wide range of hosts, a large area of ​​occurrence, and strong adaptability. They cause serious losses to agricultural production and are difficult to control. In the past, it mainly relied on the use of a large amount of chemical pesticides to prevent and control plant diseases, but it had a very large negative impact on the environment and human health. In recent years, due to the advantages of microbial pesticides with broad antibacterial spectrum and diversified active functions, more attention has been paid to biological pesticides. Antibacterial proteins have the characteristics of small relative molecular ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/00C07K1/36C07K1/30C07K1/18A01N63/02A01P3/00C12R1/445
Inventor 任建军李永平李秋水张长现梁硕何文豪
Owner BEIJING GUANGHE BIOTECH CO LTD
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