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High-affinity penicillin binding protein and application thereof

A technology for binding proteins and penicillin, which can be used in chemical instruments and methods, biochemical equipment and methods, material testing products, etc., and can solve problems such as insufficient affinity.

Inactive Publication Date: 2012-08-08
PINGYUAN COUNTY WEIFENG YONGZHU TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the known affinity between penicillin-binding proteins and β-lactam antibiotics does not meet the detection standards required by the state. In order to achieve rapid detection of β-lactam antibiotics, it is urgent to find a protein with higher affinity for β-lactam antibiotics. penicillin binding protein

Method used

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  • High-affinity penicillin binding protein and application thereof
  • High-affinity penicillin binding protein and application thereof
  • High-affinity penicillin binding protein and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0023] Example Colloidal gold test strip assembly

[0024] A kind of β-lactam antibiotic quick detection test strip comprises: bottom plate, sample pad, colloidal gold pad, nitrocellulose membrane, absorption pad, detection line, quality inspection line (see attached image 3 ). There is colloidal gold-labeled WFYZH-Pro-β-lactam on the colloidal gold pad, and there are detection lines and quality inspection lines on the nitrocellulose membrane. There are ampicillin-conjugated antigens on the detection line and WFYZH-Pro-β on the quality control line -monoclonal antibody to lactam.

[0025] A kind of preparation method of β-lactam antibiotic quick detection test strip comprises:

[0026] (1) Preparation of WFYZH-Pro-β-lactam

[0027] For details of the preparation process, see "Specific Embodiments".

[0028] (2) Preparation of antigen coupled with bovine serum albumin and ampicillin

[0029] 25 mg ampicillin dissolved in pH 9.6 Na 2 CO 3 In the buffer solution, after mi...

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Abstract

The invention provides a high-affinity penicillin binding protein. The invention is characterized in that the high-affinity penicillin binding protein is obtained by respectively transforming isoleucine 526 and glutamine 547 in a streptococcus agalactiae penicillin binding protein (PBP 1b) into lysine and arginine, so that the affinity of beta-lactam antibiotics is improved. The invention furtherprovides a quick detection test strip produced based on the protein, which can be used for quickly detecting beta-lactam antibiotic residues in agricultural and sideline products.

Description

technical field [0001] The invention relates to the field of food safety detection. More specifically, the present invention relates to a high-affinity penicillin-binding protein obtained by molecular transformation and its application in the rapid detection of β-lactam antibiotic residues in agricultural by-products. Background technique [0002] Dairy cows are susceptible to diseases such as mastitis and vaginitis during breeding. Due to the high efficacy and low price of β-lactam antibiotics, dairy farmers use a large amount of them when cows are sick, resulting in the residue of β-lactam antibiotics in raw milk The phenomenon is common, which greatly increases the difficulty of milk source quality and safety control for dairy companies, and dairy products containing β-lactam antibiotic residues will affect the health and life safety of consumers. Therefore, the Ministry of Agriculture issued the industry standard "Pollution-free Food Fresh Milk" (NY5045-2008), requiring...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/315C12N1/21G01N33/68C12R1/19
Inventor 侯伟周峰邢媛
Owner PINGYUAN COUNTY WEIFENG YONGZHU TECH
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