Kit for detecting beta-lactam antibiotic ligand in milk by receptor method and detection method thereof

Abstract

The invention discloses a kit for detecting beta-lactam antibiotic ligand in milk by a receptor method and a detection method thereof. The kit comprises the following components: (1) an enzyme-labeled plate which is coated by penicillin-binding protein PBP2xa or PBP2xb recombinant protein; (2) an enzyme-labeled marker; (3) standard solution of ampicillin sodium; (4) 20 times concentrated cleaning buffer solution; (5) enzyme-labeled diluent; (6) 20 times concentrated sample extract; (7) developing solution; and (8) reaction stop solution. The kit of the invention can simultaneously screen eight beta-lactam antibiotic residues in the milk; the detection limit is lower than the minimum residue limit of China and main developed countries in the world; the detection time is within 50 minutes; and the kit has the characteristics of simple, rapid and accurate operation, low cost and the like and is suitable for large-scale popularization and application.

Description

A kind of β-lactam antibiotic ligand receptor method detection kit and detection method in milk technical field The invention belongs to the field of micro-analysis of biochemistry, and in particular relates to a detection kit and a detection method of β-lactam antibiotic ligand receptor method in milk. Background technique With the increasing awareness of food safety, the problem of antibiotic residues in milk has attracted more and more attention from consumers. Residues in milk are mainly caused by antibiotic residues in cow feed, cow drug injection and milk insurance additives. There are many kinds of antibiotics used in veterinary clinics, among which β-lactam antibiotics are the most common, and the residues of β-lactam antibiotics are the most harmful to human body. At present, controlling the amount of antibiotics used in milk and formulating a standard method for rapid detection of antibiotic content in milk is a necessary guarantee to ensure the quality and saf...

AI Technical Summary

Problems solved by technology

Its principle is the specific recognition reaction between ligands and receptors. It has the characteristics of strong specificity, high sensitivity, short detection time, and does not require complicated equipment. However, the disadvantage is that there are few such products on the market at present, and the The types of β-lactam antibiotics that can be detected are not enough, and the detection limit is higher than the relevant residue limit standards at home and abroad, which cannot fully meet the needs of daily residue monitoring

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