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Monoclonal antibody against pbp2a derived from mrsa with dual binding activities

A monoclonal antibody, binding activity technology, applied in antibacterial immunoglobulins, instruments, depsipeptides, etc., can solve problems such as low affinity and drug resistance

Inactive Publication Date: 2013-01-23
RAYBIOTECH INC GUANGZHOU
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

This bacterium is widely resistant to a variety of commonly used antibiotics, including methicillin, and its main drug resistance mechanism is due to the penicillin binding protein 2a (Penicillin Binding Protein 2a, PBP 2a) produced by MRSA bacteria, with a protein molecular weight of 75kDa; The protein is encoded by the bacterial chromosome, i.e. the mecA gene. A large number of experiments have confirmed that PBP2a exhibits low-affinity binding to β-lactam antibiotics, leading to drug resistance

Method used

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  • Monoclonal antibody against pbp2a derived from mrsa with dual binding activities
  • Monoclonal antibody against pbp2a derived from mrsa with dual binding activities
  • Monoclonal antibody against pbp2a derived from mrsa with dual binding activities

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Embodiment 1

[0062] Figure 1. The primary antibody used in western blotting is purified mouse anti-MRSA antibody (2 μg / ml), the secondary antibody is HRP-labeled anti-mouse secondary antibody (1:5000), and then the specific protein bound to the antibody is detected by a chemiluminescent system. The arrows in the figure indicate that the antibody specifically recognizes and binds to the target protein in the bacterial sample.

[0063]

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Abstract

Mouse monoclonal antibodies specifically recognizing the Penicillin Binding Protein 2a (PBP2a) derived from a strain of Methicillin-Resistant Staphylococcus aureus (MRSA) were produced and characterized. The immunogen used to generate an immune response in a mouse was a PBP2a recombinant protein derived from a strain of Methicillin-Resistant Staphylococcus aureus (MRSA). The data showed that both monoclonal antibodies of the disclosure were able to distinguish MRSA from MSSA bacteria. The monoclonal antibodies have distinct recognition patterns for the regions of the PBP2a protein sequence. Epitope mapping has localized regions of the PBP2a protein specifically recognized by one or both of the monoclonal antibodies. The monoclonal antibodies of the present disclosure having the ability to distinguish between MRSA and MSSA strains can be useful as the basis for a diagnostic assay useful in the clinical setting for determining whether and which antibiotics to administer to a patient.

Description

Technical field: [0001] The invention belongs to the technical field of monoclonal antibodies (technical field of biomedicine), in particular, a monoclonal antibody capable of specifically recognizing epitopes of Staphylococcus aureus (MRSA) and capable of distinguishing MRSA and MSSA has been confirmed by experiments. Background technique: [0002] MRSA (methicillin-resistant Staphylococcus aureus), that is, methicillin-resistant Staphylococcus aureus, is one of the most important drug-resistant pathogens causing infection in patients worldwide. This bacterium is widely resistant to a variety of commonly used antibiotics, including methicillin, and its main drug resistance mechanism is due to the penicillin binding protein 2a (Penicillin Binding Protein 2a, PBP 2a) produced by MRSA bacteria, with a protein molecular weight of 75kDa; The protein is encoded by the bacterial chromosome, that is, the mecA gene. A large number of experiments have confirmed that PBP2a exhibits lo...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/12C07K7/08C07K14/31G01N33/577
CPCC07K7/08G01N33/577C07K2317/33C07K16/1271C07K16/12G01N33/56938C07K14/31
Inventor 黄若磐张英
Owner RAYBIOTECH INC GUANGZHOU
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