Modeling method of non-rodent animal model for studying intestinal flora

A modeling method, the technology of intestinal flora, applied in the field of intestinal symbiotic bacteria research, can solve the problems such as no successful reports, and achieve the effect of stable model, short time and low mortality

Inactive Publication Date: 2018-04-13
INST OF MEDICAL BIOLOGY CHINESE ACAD OF MEDICAL SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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  • Modeling method of non-rodent animal model for studying intestinal flora
  • Modeling method of non-rodent animal model for studying intestinal flora
  • Modeling method of non-rodent animal model for studying intestinal flora

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Experimental program
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Embodiment 1

[0024] Establishment, evaluation and analysis of a non-rodent animal model for the study of gut commensal flora.

[0025] 1. Construct a primate model in which the intestinal commensal flora has been cleared

[0026] 1.1 Take 12-month-old rhesus monkeys as experimental animals, 2 males (numbered 1 and 2 respectively), with a body weight of 3kg±0.5kg. Raised in a barrier environment, fed the same diet. The daily food needs are sterilized by autoclaving and consumed within 72 hours; the drinking water is sterilized by autoclaving and consumed within 24 hours.

[0027] 1.2 Preparation of mixed antibiotics: the antibiotics used include aminoglycosides, penicillins, glycopeptides and nitroimidazole antibiotics, including neomycin, streptomycin, ampicillin sodium, kanamycin, metronidazole and Vancomycin, both in powder form. The dosages are: neomycin 60mg / kg / day, streptomycin 60mg / kg / day, ampicillin sodium 60mg / kg / day, kanamycin 50mg / kg / day, metronidazole 60mg / kg / day , Vancomyci...

Embodiment 2

[0069] Repeat Example 1, only the number of rhesus monkeys and the antibiotic formula in step 1.2 are different:

[0070] The numbers of the two rhesus monkeys in this embodiment are 3 and 4 respectively.

[0071] 1.2 Preparation of mixed antibiotics: the antibiotics used include aminoglycosides, penicillins, glycopeptides and nitroimidazole antibiotics, including neomycin, streptomycin, ampicillin sodium, kanamycin, metronidazole and Vancomycin, both in powder form. The dosages are: neomycin 60mg / kg / day, streptomycin 60mg / kg / day, ampicillin sodium 60mg / kg / day, kanamycin 50mg / kg / day, metronidazole 60mg / kg / day , Vancomycin 60mg / kg / day. The average dose was divided into 3 times.

[0072] 1.2.1 Antibiotic formula 1: Weigh the corresponding weights of neomycin, streptomycin, ampicillin sodium and kanamycin respectively and dissolve them in 5% sterile sucrose water, mix them well and put them in the medicine tube ; Another corresponding weight of vancomycin was dissolved in 5% ...

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Abstract

The invention discloses a modeling method of a non-rodent animal model for studying intestinal flora. A male rhesus monkey of 12-months old is taken as a laboratory animal and fed with mixed antibiotic for 21 days to clear symbiotic intestinal flora in the body. The antibiotic includes neomycin, streptomycin, ampicillin sodium, kanamycin, metronidazole and vancomycin; different antibiotic formulasare adopted for the first 10 days and later 11 days of feeding. The modeling result is verified by combining a Realtime-PCR method and an amplicon sequencing method. The method disclosed by the invention has the advantages of simplicity and easiness in implementation, short time, high repeatability, low death rate and stable model. The method has an important application value in the treatment offlora-related metabolic diseases, inflammatory bowel diseases and infectious diseases, evaluation of intestinal flora in the immune system, development of new medicines and the like.

Description

technical field [0001] The invention belongs to the technical field of intestinal symbiotic bacteria research, and in particular relates to a modeling method of a non-rodent animal model used for the research of intestinal symbiotic flora. Background technique [0002] The digestive tract of mammals is colonized with a large number and variety of symbiotic flora. The relationship between them and the host is intricate and they are an integral part of the body. With the development of sequencing technology, the relationship between symbiotic flora and host health has become increasingly clear, and the changes and roles in the occurrence and development of human diseases have gradually become clear. Among them, the intestinal tract is the site with the highest abundance of symbiotic bacteria, the most vigorous metabolism, and the most researched sites. Intestinal symbiotic bacteria play an important role in the development of the body's immune system, digestion and metabolism...

Claims

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Application Information

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IPC IPC(8): A61K31/7036A61K31/4164A61K38/14A61K31/43C12Q1/6869C12Q1/686C12Q1/04
CPCA61K31/4164A61K31/43A61K31/7036A61K38/14C12Q1/686C12Q1/6869A61K2300/00C12Q2535/122C12Q2561/113
Inventor 刘龙丁李洪哲王晶晶李恒郭磊黄星范海涛杨泽宁郑惠文储曼曼
Owner INST OF MEDICAL BIOLOGY CHINESE ACAD OF MEDICAL SCI
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