Self-assembly antibacterial peptide

A self-assembly, antibacterial peptide technology, applied in the direction of antibacterial drugs, peptides, peptide/protein components, etc., can solve problems such as insufficient depth, and achieve the effects of increasing water solubility, good self-assembly ability, excellent antibacterial activity and selectivity

Active Publication Date: 2016-07-13
CHINA UNIV OF PETROLEUM (EAST CHINA)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at this stage, people do not have a deep understanding of the relationship between the structure and activity of self-assembled antimicrobial peptides.

Method used

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  • Self-assembly antibacterial peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Synthesis of self-assembling antimicrobial peptides:

[0028] 1. Materials

[0029] (1) Take by weighing MBHA resin (toluene hydrogenamine resin) 0.7861g;

[0030] (2) Prepare a DMF (dimethylformamide) solution of the following substances at a concentration of 0.2mol / L:

[0031] Fmoc-Phe-OH (N-fluorenylmethoxycarbonyl-phenylalanine): volume 21mL, mass 1.63g;

[0032] Fmoc-Lys(Boc)-OH(N-fluorenylmethoxycarbonyl-N'-tert-butoxycarbonyl-lysine): volume 21mL, mass 1.97g;

[0033] Fmoc-Pro-OH (N-fluorenylmethoxycarbonyl-proline): volume 11mL, mass 0.74g;

[0034] Fmoc-Leu-OH (N-fluorenylmethoxycarbonyl-leucine): volume 21mL, mass 1.48g;

[0035] Fmoc-Gly-OH (N-fluorenylmethoxycarbonyl-glycine): volume 11mL, mass 0.65g;

[0036] Fmoc-Ala-OH (N-fluorenylmethoxycarbonyl-alanine): volume 11mL, mass 0.68g;

[0037] Fmoc-Arg(Pbf)-OH(N-Fremoxycarbonyl-2,2,4,6,7-pentamethyldihydrobenzofuran-5-sulfonyl-arginine): volume 11mL, mass 1.43g;

[0038] Nap-CH 2 COOH: volume 11mL, m...

Embodiment 2

[0048] Self-assembled morphology and secondary structure of self-assembled antimicrobial peptides

[0049] (1) Prepare the phosphate buffer solution (pH7.0, ionic strength 10mM) of the polypeptide molecule Nap-Phe-Phe-Lys-Pro-Leu-Gly-Leu-Ala-Arg-Lys, and its concentration is 0.25mM, 0.5mM respectively mM, 1.0mM and 2.0mM, placed at room temperature for 3 days, observed circular dichroism spectrum results and atomic force microscope results.

[0050] The results of the circular dichroism spectrum are as follows figure 1 As shown in a, it is found that the polypeptide molecule mainly presents a random coil secondary structure when the concentration is below 1.0 mM; at a concentration of 2.0 mM, it presents a β-sheet secondary structure.

[0051] Atomic force microscopy results such as figure 1 b. figure 1 As shown in c, it was found that under the concentration of 1.0mM polypeptide molecules, there were many random aggregates in the solution, but no regular self-assembly stru...

Embodiment 3

[0056] Cell / bacterial culture test

[0057] (1) Cell experiment: first inoculate 100 μl in a sterile 96-well plate with a density of 1×10 5 Cells / mL HeLa / NIH3T3 cells were placed in a 37°C incubator for 24 hours. After they adhered to the wall, the culture solution in the well plate was sucked out, and 100 μl of fresh culture solution and 100 μl of filtered different concentrations of Polypeptide solution, its absorbance is Abs peptide , 4 parallels were set up for each concentration, and the wells that only added Tris (trishydroxymethylaminomethane) buffer and no peptide were used as a control group, and the absorbance was Abs Tris , and then put the well plate back in the incubator at 37°C for 6h. After the effect was completed, 20 μl of MTT (3-(4,5-dimethylthiazole-2)-2 at a concentration of 5 mg / mL was added to each well. , 5-diphenyltetrazolium bromide) solution, continue culturing in the incubator for 4 hours, then suck out the liquid in the well plate, add 150 μl of d...

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Abstract

The invention provides self-assembly antibacterial peptide, and belongs to the field of self-assembly polypeptide.The sequence of the antibacterial peptide is Nap-Phe-Phe-Lys-Pro-Leu-Gly-Leu-Ala-Arg-Lys (Nap represents a naphthyl group, Phe represents phenylalanine, Lys represents lysine, Pro represents proline, Leu represents leucine, Gly represents glycine, Ala represents alanine and Arg represents arginine), and the antibacterial peptide is cationic amphiphilic oligopeptide.The antibacterial peptide has good self-assembly capacity, and the self-assembly behaviors present enzyme responsiveness.The antibacterial peptide has no toxicity to Gram negative bacteria, human cells and animal cells within a specific concentration range (50-150 micrometers), has powerful selective killing capacity only for Gram positive bacteria and has good antibacterial activity and selectivity in other words.

Description

technical field [0001] The invention belongs to the field of self-assembled polypeptides, in particular to a cationic amphiphilic self-assembled antibacterial peptide. Background technique [0002] Since the discovery of penicillin, antibiotics have been a powerful weapon for humans to treat diseases caused by pathogenic microorganisms. However, because traditional antibiotics mainly exert antibacterial effects by interfering with bacterial cell walls, DNA or protein biosynthesis, they are prone to produce drug resistance of pathogenic bacteria and suppress immune function, which seriously affect the therapeutic effect. Therefore, it is urgent to develop new antibiotics. Antimicrobial peptides are a class of small molecule polypeptides, generally consisting of no more than 60 amino acid residues. Most of these active peptides have the characteristics of strong alkalinity, thermal stability, and broad-spectrum antibacterial activity, and have extensive inhibitory effects on ...

Claims

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Application Information

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IPC IPC(8): C07K7/06A61K38/08A61P31/04
CPCC07K7/06A61K38/00Y02A50/30
Inventor 曹美文徐海王继乾王宁宁谢子龙赵文婧
Owner CHINA UNIV OF PETROLEUM (EAST CHINA)
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