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Gene and chemical drug cotransporters and methods of use

A gene and drug technology, applied in the field of gene and chemical drug co-transporter, can solve the problems of cumbersome synthesis steps, difficult to decompose, poor biocompatibility of lipid molecules, etc., and achieve the effect of simple synthesis process

Active Publication Date: 2021-12-10
CHINA UNIV OF PETROLEUM (EAST CHINA)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, most of the drug carriers that have been studied are single drug carriers, and there are few studies on co-transporters, and most of them are surface-modified lipid molecules, which can provide hydrophobic cavities and DNA binding sites. However, these lipid molecules serve as There are still two outstanding problems in drug carriers: first, the surface modification process of lipid molecules is complicated, and the synthesis steps are cumbersome; second, lipid molecules have poor biocompatibility and are not easy to be decomposed in vivo

Method used

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  • Gene and chemical drug cotransporters and methods of use
  • Gene and chemical drug cotransporters and methods of use
  • Gene and chemical drug cotransporters and methods of use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Polypeptide molecular solution preparation:

[0042]Prepare a peptide molecule Ac-IIIIIIGPLGLAGGRRRRRRR-NH2 solution with a concentration of 4.0mM, dissolve it in Hepes buffer with a pH of 7.4 and a concentration of 20mM, and leave it for more than 3 days. It self-assembles into a nanofibrous structure, and its two The order structure and its morphology were characterized by transmission electron microscopy and atomic force microscopy. The results are as follows figure 2 As shown, a fibrous structure with a diameter of 5-8 nm and a length of more than 1 μm is formed;

[0043] Preparation of peptide / drug molecule carrier solution: Prepare a mixed solution of peptide molecules and Nile red molecules, fix the peptide concentration at 4.0mM, add Nile red at a molar ratio of 5%, ultrasonicate for 30 minutes, and centrifuge to remove the untreated solution at the bottom of the solution after three days. Loaded Nile Red, calculated to determine that the saturated load is abo...

Embodiment 2

[0049] The experimental procedure is the same as in Example 1, the difference is that in the preparation step of the polypeptide / drug molecule carrier solution, the polypeptide molecule Ac-IIIIGPLGLAGGRRRRRRR-NH 2 The concentration of the peptide was adjusted to 8.0mM, and the ratio of the positive charge of the peptide molecule to the negative charge of the gene molecule was adjusted to 20.0 in the preparation step of the peptide / drug / gene molecule carrier solution. The system can also successfully simulate the drug molecule (Nile Red) and Co-transport and efficient gene transfection of green fluorescent protein plasmid (pEGFP-N2).

Embodiment 3

[0051] The experimental procedure is the same as in Example 1, the difference is that in the preparation step of the polypeptide / drug molecule carrier solution, the polypeptide molecule Ac-IIIIGPLGLAGGRRRRRRR-NH 2 The concentration of the peptide was adjusted to 2.0mM, and the ratio of the positive charge of the peptide molecule to the negative charge of the gene molecule was adjusted to 10.0 in the preparation step of the peptide / drug / gene molecule carrier solution. The system can also successfully realize the simulation of drug molecules (Nile Red) and Co-transport and efficient gene transfection of green fluorescent protein plasmid (pEGFP-N2).

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Abstract

The invention proposes a gene and chemical drug co-transport carrier and its use method, belonging to the field of biological materials, the carrier can co-transport gene drug and chemical drug, and the load of hydrophobic drug does not affect the self-assembly structure of polypeptide and the combination with DNA Ability to simultaneously deliver simulated hydrophobic drug molecules and DNA into cells. The technical scheme includes dissolving polypeptide molecules in a buffer solution to self-assemble into a nanofibrous structure, first fully combining with hydrophobic drug molecules or simulated drug molecule solutions to obtain a polypeptide / drug molecule carrier solution, and then dissolving the polypeptide / drug molecule The molecular carrier solution and the gene molecule solution are fully mixed under predetermined conditions, that is, the polypeptide / drug / gene molecule carrier solution. The invention can be applied in the process of co-transport of genes and chemical drugs.

Description

technical field [0001] The invention belongs to the field of biological materials, in particular to a gene and chemical drug co-transport carrier and its application method. Background technique [0002] Due to the emergence of organisms' resistance to chemotherapeutic drugs, a single drug often cannot obtain the best curative effect in the treatment of diseases. Therefore, multi-drug combination therapy has become a hot spot in drug research in recent years. At present, combined drug therapy mainly includes different chemotherapy combinations, among which the combined therapy of small molecule chemotherapeutic drugs and gene drugs has received more and more attention. However, its development is hampered by a major factor, namely, the construction of drug delivery systems, the key of which lies in the design of simple co-transport drug carriers. [0003] For a molecule to be a gene-drug co-transporter, it must have two functions: first, as a gene carrier, it should be an e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/64A61K9/08A61K31/704A61K31/713A61K31/538A61K45/06
CPCA61K45/06A61K47/64A61K9/0019A61K9/08A61K31/538A61K31/704A61K31/713A61K2300/00
Inventor 曹美文赵文婧卢沙王瑜王生杰夏永清
Owner CHINA UNIV OF PETROLEUM (EAST CHINA)
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