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This invention provides a rumen methane inhibitor and its application, belonging to the field of ruminant farming. The active ingredients of the rumen methane inhibitor of this invention include: *Lactobacillus reuteri* inoculum, *Lactobacillus gasseri* inoculum, *Lactobacillus casei* inoculum, amylase, phytase, xylanase, cellulase, and β-glucanase. The rumen methane inhibitor can effectively regulate the intestinal microbiota of dairy cows, promote the decomposition of fibrous material in the rumen, optimize the rumen fermentation environment, thereby controlling rumen function, reducing methane production, and improving the productivity of ruminants. The rumen methane inhibitor is safe and environmentally friendly, which is conducive to its application in ruminant farming, agriculture, and other fields, and has good practicality.