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Application of ANO1 protein in prediction on prognosis of esophagus cancer and precancerous lesion risk

A technique for predicting precancerous lesions and risks, applied in the field of kits or detection reagents

Active Publication Date: 2015-03-18
CANCER INST & HOSPITAL CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, none of these molecules has so far been clinically accepted for use
The research on the prediction of cancer risk for people with precancerous lesions in high-occurrence sites is still in the initial stage of exploration

Method used

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  • Application of ANO1 protein in prediction on prognosis of esophagus cancer and precancerous lesion risk
  • Application of ANO1 protein in prediction on prognosis of esophagus cancer and precancerous lesion risk
  • Application of ANO1 protein in prediction on prognosis of esophagus cancer and precancerous lesion risk

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0083] Retrospective study: 359 cases of esophageal squamous cell carcinoma and their corresponding surgical incision tissues were prepared into tissue microarrays, in which 3 spots were taken for tumor tissue and 2 spots were taken for surgical incision tissue. The expression of ANO1 protein was detected by immunohistochemical method, scored and analyzed. Table 1 lists the specific detection results of ANO1 immunohistochemistry, figure 1 It is a representative picture of immunohistochemical detection results. Kaplan-Meier method and Log-rank test were used for survival analysis, and the results showed that patients with positive expression of ANO1 protein had a shorter survival time after radical surgery than those without expression (such as figure 2 shown). Table 2 shows the results of multivariate COX regression analysis based on the data of radical resection cases in Table 1. It can be seen that the high expression of ANO1 and lymph node metastasis can be used as indep...

Embodiment 2

[0100] Retrospective study: 12 cases of endoscopic biopsy pathologically diagnosed as esophageal squamous cell carcinoma specimens and 75 cases of endoscopic biopsy pathologically diagnosed as esophageal normal epithelial specimens were prepared into large tissue pieces, and the expression level of ANO1 protein was detected by immunohistochemical method , and analyze it. The result is as image 3 As shown, ANO1 protein was not expressed in normal esophageal population, with a specificity of 100%; it was positively expressed in some patients with esophageal squamous cell carcinoma, with a sensitivity of 25% (3 / 12). Table 3 lists the specific test results.

[0101] Table 3 Immunohistochemical detection results of ANO1 protein in endoscopic biopsy esophageal squamous cell carcinoma and normal population specimens

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Embodiment 3

[0106] Retrospective study: 168 cases of endoscopic biopsy of esophageal atypical hyperplasia were prepared into large tissue pieces, and the expression level of ANO1 protein was detected and analyzed by immunohistochemical method. The result is as Figure 4 As shown, ANO1 protein was positive in 1 patient with mild dysplasia; positive in 1 patient with severe dysplasia. Table 4 lists the specific test results.

[0107] Table 4 Immunohistochemical detection results of ANO1 protein in endoscopic biopsy specimens of esophageal atypical hyperplasia at high detection sites

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Abstract

The invention belongs to the field of prognosis estimation and canceration risk prediction on clinic of molecular biology, and particularly relates to application of a molecule of a detection ANO1 protein in the preparation of a kit or a detection agent for the prognosis estimation and precancerous disease progression risk prediction of esophageal squamous carcinoma. The molecule expressed by the detection ANO1 protein can be a nucleic acid, a protein or a compound, thus showing that the ANO1 protein can be used as a molecular marker for the prognosis estimation and precancerous disease progression risk prediction of the esophageal squamous carcinoma.

Description

technical field [0001] The invention belongs to the fields of molecular biology, clinical prognosis judgment and cancer risk prediction, and in particular relates to the detection of ANO1 protein molecules in the preparation of kits or detection reagents for the prognosis of esophageal squamous cell carcinoma and the prediction and judgment of the disease course progression risk of precancerous lesions. application. The present invention also relates to kits or detection reagents comprising molecules for detecting ANO1 protein. Background technique [0002] Esophageal cancer is one of the ten most common malignant tumors in human beings. Globally, there are more than 482,000 new cases of esophageal cancer and more than 407,000 deaths each year. China is a high-incidence area of ​​esophageal cancer in the world, especially in men. The latest data from the "China Cancer Registry Annual Report" published in 2012 shows that esophageal squamous cell carcinoma ranks fifth in the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/57484G01N33/6893G01N2800/50
Inventor 王明荣商利张钰郝佳洁徐昕蔡岩
Owner CANCER INST & HOSPITAL CHINESE ACADEMY OF MEDICAL SCI
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