Probe of human papillomavirus and DNA chip comprising the same

Abstract

Oligonucleotide probes for analyzing 40 types of HPV were synthesized, and DNA chips were produced by using the oligonucleotide probes. The synthesis of the oligonucleotide probes is based on clones of Ll and E6 / E7 genes of 35 types of HPV obtained from cervical cell specimens from 4,898 Korean adult women and tissue specimens from 68 cervical cancer cases in addition to information based on American and European cases. The DNA chips can analyze the 40 types of HPV found in cervical, diagnose complex infection by at least one type of HPV, and have excellent diagnostic sensitivity and specificity on HPV genetic type up to 100% and reproducibility. Also, the DAN chips are superior to conventional analytic method, and very economical, since they can analyze numerous specimens in shortest time. Accordingly, the DNA chips are useful for predicting cervical cancer and precancerous lesion.

Description

technical field

[0001] The present invention relates to probes that bind complementary to the nucleic acid of human papillomavirus (HPV), which is the main cause of cervical cancer and the most prevalent sexually transmitted disease, and to DNA chips or microchips containing the probes. Arrays, diagnostic kits for genotyping HPV, and methods for testing for the presence and genotyping of HPV infection. Background technique

[0002] Human papillomavirus (HPV) is a virus with a double-stranded DNA genome surrounded by a capsid, which is shaped like a golf ball. HPV is important to humans in two ways. First, HPV is the most common sexually transmitted disease (STD). It is reported that 50% or more of all adult women will be infected with HPV at least once in their lifetime. Second, HPV infects human epithelial cells, inducing hyperproliferation. In most cases, this hyperplasia is a benign tumor such as simple skin warts, genital warts around the external genitalia or anus, ...

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