Preparation method of electrochemical sensor for detection of heavy metal lead contaminants

A biosensor and heavy metal technology, applied in the field of electrochemical detection, can solve the problems of high cost, achieve the effect of facilitating commercialization, promoting development, and improving catalytic performance

Active Publication Date: 2018-01-23
CHONGQING MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods require expensive equipment and materials, and the tested samples must undergo complex pretreatment to eliminate other interferences. In addition, they must be analyzed by technicians in specialized laboratories

Method used

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  • Preparation method of electrochemical sensor for detection of heavy metal lead contaminants
  • Preparation method of electrochemical sensor for detection of heavy metal lead contaminants
  • Preparation method of electrochemical sensor for detection of heavy metal lead contaminants

Examples

Experimental program
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Embodiment 1

[0041] Step 1. Add 2mg of reduced graphene oxide rGO-TEPA into 1mL of ultrapure water, sonicate in an ultrasonic instrument for 1 hour, then transfer to a 5mL beaker, mix with 1.45mL of 0.8M AA aqueous solution, 0.5mL of 2.0M PVP aqueous solution, 1mL2% HAuCl4 mixed, heated to 90 ° C, vigorously stirred for 3 hours to obtain a reduced graphene oxide-gold nanoparticles (rGO-TEPA-Au) mixed solution;

[0042] Step 2. Use 0.3 and 0.05 μm Al respectively 2 o 3 Polish the electrode into a mirror surface with powder, then ultrasonically ultrasonicate the electrode for 5 minutes each in the order of ultrapure water, absolute ethanol, and ultrapure water, and dry at room temperature for later use;

[0043] Step 3. Add 10 μL of electrode modification material reduced graphene oxide tetraethylenepentamine-gold nanoparticles (rGO-TEPA-Au) dropwise on the surface of the electrode, and dry at room temperature;

[0044] Step 4. Rinse the electrode with ultrapure water and add 10 μL, 100 μg...

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Abstract

The invention relates to a preparation method and application of an electrochemical sensor for detection of heavy metal lead contaminants and belongs to the technical field of electrochemical detection. The preparation method is characterized by including; performing synthesis to obtain a Fe-MOFs nano material, reducing palladium-platinum bimetallic nanoparticles onto the Fe-MOFs nano material, and mixing a hairpin type DNA signal probe with the composite material to obtain a biological signal probe; performing layer-by-layer self-assembly through rGO-TEPA and avidin for fixing of '8-17'DNAzyme to obtain the electrochemical sensor for detection of the heavy metal lead contaminants. The sensor is successfully applied to detection of lead contaminants in the environment. The preparation method and application has the advantages of high flexibility, high specificity and rapid and convenient detection; experimental evidence is provided for research of lead contamination detection technology, and new concepts and new technology platforms are provided for monitoring the lead contaminants in the environment are provided.

Description

Technical field: [0001] The invention relates to a preparation method and application of an electrochemical sensor for quantitatively detecting heavy metal lead ions in the environment, especially a biosensor based on a metal-organic framework and a bimetallic palladium-platinum nanocomposite material as a signal probe, and uses "8 The specific catalytic function of -17"DNAzyme is used to detect heavy metal lead pollutants, which belongs to the field of electrochemical detection. Background technique: [0002] With the growth of the national economy, the serious damage to the ecological environment has attracted more and more attention from the government departments. In particular, heavy metal pollution is still a global problem, and the hazards to residents' public health are becoming increasingly apparent. Among them, lead is a heavy metal element with accumulation and multi-affinity, which is extremely harmful to living organisms, especially children. Excessive lead ac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/416
Inventor 何俊琳于超余雨洁
Owner CHONGQING MEDICAL UNIVERSITY
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