41results about How to "Same conditions" patented technology

The invention discloses a human leukocyte antigen HLA-A and HLA-B gene full-length sequencing method and an HLA gene sequencing and typing method. The HLA-A and HLA-B gene full-length sequencing method comprises the following steps of: a, performing PCR amplification on about 4kb full-length sequences of HLA-A and HLA-B genes by using a pair of primers respectively; and b, cloning the amplification products to a pGEM-Tea sy vector, sequencing the full-length sequences by using ten walking sequencing primers in positive and negative directions respectively, and totally obtaining 38 allele 4.3kb full-length sequences of the HLA-A and 30 allele 3.7kb full-length sequences of the HLA-B. The HLA-A and HLA-B sequencing and typing method comprises the following steps of: performing PCR amplification on typing target areas of the HLA-A and HLA-B by using two pairs of primers respectively; and performing two directional sequencing on products by using fourteen sequencing primers respectively, wherein the HLA-DRB1 and HLA-DQB1 sequencing and typing method comprises the following steps of: amplifying sequences of second and third exons of DRB1 and DQB1 by adopting group specificity primers respectively; performing two directional sequencing on the second and third exons of the DRB1 by adopting eight group specificity primers and three sequencing primers; and performing two directional sequencing on the second and third exons of the DQB1 by adopting four sequencing primers respectively.

The invention discloses technology for producing direct spinning terylene industrial filaments after liquid state polycondensation of prepolymer and belongs to the technical field of spinning. The technology comprises the processes of esterification, prepolycondensation, liquid state polycondensation, and spinning, and is characterized by comprising the following steps of: performing prepolycondensation reaction on esterified ethylene glycol and phthalic acid to prepare a melt with the viscosity of 0.3 to 0.4dl/g, and conveying the melt to a liquid state polycondensation kettle for liquid state polycondensation by using a booster pump; and conveying the melt to a spinning beam for spinning through a gear pump, wherein the inherent viscosity of the polyester melt after liquid state polycondensation is 0.85 to 1.05dl/g. By directly conveying the melt into the liquid state polycondensation kettle for polycondensation and performing the production technology of directly spinning the melt, the production technology for producing the direct spinning terylene industrial filaments after the liquid state polycondensation of the prepolymer shortens the process flow, reduces the production energy consumption and production cost, and solves the technical problem of difficulty in conveying the melt simultaneously.

The invention relates to a double-labeling kit for detecting troponin (Tn) and a compound and preparation and detection methods of the double-labeling kit, and belongs to the field of immunodetectionanalysis technologies and nano-biotechnologies. The kit comprises a reaction buffer solution integrated on a reagent strip, a cleaning solution, an enhancement solution, a magnetic particle solution coating a cTnI monoclonal antibody, a europium-labeled cTnC monoclonal antibody solution and a samarium-labeled cTnI monoclonal antibody solution and 2-6 bottles of additional freeze-dried calibrationproducts of natural cTnI antigens (containing cTnC) with different concentrations. The kit can provide a nearly-homogeneous double-labeling reaction system to ensure that the content of cTnI with complete fragments and the cTnI-C compound is detected simultaneously, the detection time is shortened, the efficiency is improved, and convenience is brought to clinical use.

A reproducing apparatus and method for reproducing recorded information recorded on an information recording medium. A drive is provided for reproducing the recorded information from the information recording medium. A non-volatile memory is provided for memorizing at least two informations, each including an identifier of the information recording medium, and position information indicative of a position on the recording medium, where the drive is reproducing, while maintaining correspondence with each other therebetween. A controller is provided for deciding on whether the identifier is memorized or not in the non-volatile memory, which is coincident with an identifier reproduced from the information medium by the drive previously. The recorded information is reproduced from the information recording medium loaded onto the drive.

The invention discloses a method of synthesizing GHK acetate at low cost. The method includes steps of: 1) performing a reaction to Trt-Gly-OH with N-hydroxysuccinimide to generate Trt-Gly-OSu; 2) performing a reaction with H-His(Trt)-OH to generate Trt-Gly-His(Trt)-OH; 3) performing a reaction with N-hydroxysuccinimide to generate Trt-Gly-His(Trt)-OSu; 4) performing a reaction with Lys(Trt)-OH togenerate Trt-Gly-His(Trt)-Lys(Trt)-OH, and removing protective groups from the Trt-Gly-His(Trt)-Lys(Trt)-OH in acetic acid to generate the GHK acetate. In the method, all the protective groups are triphenylmethyl group, so that optical purity is guaranteed well; deprotection can be directly carried in acetic acid, so that byproducts generated during deprotection of trifluoroacetic acid can be avoided. The GHK acetate is free of reverse phase chromatograph for purification and ion exchange chromatography for remove trifluoroacetic acid, so that production cost is reduced largely and the methodcan be used in large-scale production.

The invention discloses an artificial climate simulation test box, which comprises an environmental gas preparation and humidity control unit and an environmental simulation unit, can regulate and control conditions such as spectral distribution, illuminance, irradiance, air temperature, humidity, wind power, underground water level burial depth, soil temperature and the like in a simulation testbox body, and can realize indoor artificial simulation of a natural environment. An external gas generation chamber is matched with a fin cold and heat source medium temperature control module to realize temperature and humidity regulation and control; frequent starting and stopping, and sequential starting and stopping of electric heating, electric refrigeration and dehumidification equipment areavoided, the adverse effects of residual heat and residual cold left after the equipment is powered off on air temperature and humidity control in a simulation test box body are eliminated, and highprecision and low fluctuation of air temperature and humidity regulation and control in the simulation test box body are guaranteed. Interference of air blowing equipment of a traditional artificial climate simulation box on aerodynamic conditions in the simulation test box body is avoided, and single-variable and combined-variable research on wind power conditions can be achieved.

A paper insertion limiting device of a paper feeding unit which prevents papers from being inserted to a drive roller when the papers are loaded. The paper insertion limiting device of a paper feeding unit is provided with a sheet regulating member mounted on the front end of the paper feeding unit to rotate by a certain angle, and regulate an insertion depth of papers loaded in the paper feeding unit, and a regulating member activator to actuate the sheet regulating member to block the front ends of papers when the carrier reaches a certain position, and to feed papers when the carrier moves out of the certain position. The sheet regulating member forms a right angle to the face of papers when the carrier reaches the certain position, and the paper regulation member forms an obtuse angle to the papers when the carrier moves out of the certain position.

The invention discloses a production method of melt direct spinning flat polyester industrial filaments. A spinneret plate with special '-' shaped section spinneret orifice is applied. Products are made by tackifying of molten fluid, melt spinning and tension winding molding. By using the production method of melt direct spinning flat polyester industrial filaments, the reaction time is decreased, technological process is effectively lessened, the efficiency is increased, energy consumption is reduced, and the cohesive property is greatly increased on the basis of keeping advantages of common industrial yarn. Because of the approximate rectangle section, the space between yarns is small, the contact area is added, the arrangement is more smooth and compact, and thus the yarn has great raw erectness and high cohesive property, relative good bending rigidity and flexibility. Because of compact arrangement of yarns and increased flatness, the aesthetics after blade coating is greatly increased, the production process is reduced and the production art is simplified.

A holding device with limitation of a moment for objects formed as piston rods to be screwed into components formed as pistons contained in an interior of syringe cylinders, the holding device comprising a holder for an object; a braking device which is formed to non-rotatably hold the holder and, through the holder, the object with a predetermined braking moment, the braking device having an electric brake which acts on the holder.

The invention provides a testing device for judging influence of different water depths on fish farming. The device comprises a combined framework (1), a mesh box (2), a connector (3), a bearing base (4), a rotating shaft (5) and a sleeve (6), wherein the combined framework (1) is composed of at least two rectangular frameworks, and every rectangular framework is mutually and vertically overlapped and connected into one; the mesh box (2) is matched with every framework and fixed at inside of the framework through a network wire; the connector (3) is fixedly connected to the upper end part of the combined framework (1), and the top end thereof is connected to the sleeve (6) and formed into an integrated structure with it; one end of the rotating shaft (5) crosses through the sleeve (6) and the fixing piece fixes both ends or one end of the sleeve thereon to form a dynamic fit; the other side is sleeved to the bearing base (4). The device is simple in structure, convenient to operate, low in cost, and flexible in use; moreover, the testing device has direct guiding significance for the best water depth of the fish farming within the given water domain scale under the natural condition.

The invention provides a portable negative pressure sensor field calibration device. A miniature pump-and-pull dual-purpose air pump is used to connect a miniature air storage tank. Through controlling the switches of four two-position two-pass solenoid valves, automatic switching of an air exhaust or pumping air channel is realized. Two kinds of work modes, which are vacuum pumping and pressurization, are established. The precision regulating valve of a tee-junction structure is used to cooperate with a high-precision digital display pressure meter so that the pressure in the air storage tankcan be accurately controlled and a 10-300KPa continuous-adjustable pressure environment is formed. All components are installed in a calibration box which has a small size and is convenient to carry.The design of the device is reasonable, operation is simple and carrying is convenient. The device is suitable for negative pressure sensor calibration in a test and industrial field environment.

The invention discloses complex heating type biological carbon production equipment with automatic temperature control. The complex heating type biological carbon production equipment with automatic temperature control comprises an induced draft fan, a motor, an external heating device, a drying and carbonization system, a flue gas purification system and a cooling and carbon discharge device; the drying and carbonization system is composed of a carbonization furnace, a combustion chamber, pyrolysis chambers, external heating devices, a temperature sensor and a flue gas exhaust pipeline; each pyrolysis chamber is fixed at the upper part of the carbonization furnace through a detachable hanging device; the upper end of each pyrolysis chamber is sealed; the lower end of each pyrolysis chamber is opened; a double-layer sealing cover plate is arranged at the bottom of each pyrolysis chamber; and a carbonization furnace chamber, the flue gas purification system and the induced draft fan are connected with one another through an exhaust pipeline. The invention also provides a complex heating type biological carbon production process with automatic temperature control. The equipment and the process method are low in energy consumption, constant in temperature in production process and rapid in pyrolysis speed, can realize batch production, saves energy and is green and environmentally friendly.

The invention relates to a balance pressurization type high efficiency digestion apparatus, which comprises a pressure vessel, a pressurization unit, a heating block, a heating resistance wire, an air cooling pipeline, an inflator, a sample pipe and outlet pipe; the pressurization unit and the pressure vessel are communicated, and the heating block is equipped in a pressure vessel, and the heating resistance wire and the heating block are connected, and the air cooling pipeline is in the heating block, and one end of the air cooling pipeline is connected to the inflator equipped outside the pressure vessel, and the other end extends to the outside part of the pressure vessel, and the sample pipe is inserted on the heating block, and one end of the outlet pipe is equipped in the pressure vessel, and the other end extends to the outside of the pressure vessel. Compared with the prior art, the invention employs a method for digesting many samples simultaneously in an integral pressurization container, thereby completing digestion front treatment of the sample, and ensuring accuracy of the test result under the same condition, and a filtering processing can be obtained by gas generated in digestion; the invention has the advantages of rapid, safety, environmental protection and energy saving.

The invention belongs to the technical field of cell observation, and particularly relates to an intelligent dyeing system and method. The system comprises a transmission part which comprises a motion transmission mechanism, a first mechanical arm and a sensor and is used for automatically putting a smear into a dyeing area and taking the smear out of the dyeing area after dyeing, a liquid dripping part which comprises a second mechanical arm, a dyeing liquid dripping pipeline, a cleaning liquid flushing pipeline and a waste liquid collecting pipeline, and is used for dripping dyeing liquid and buffer liquid to the smear and flushing redundant dyeing liquid after dyeing, a mixing part which is used for uniformly mixing the dyeing liquid and the buffer liquid on the smear, a gland part which is used for fixing and sealing the smear, a drying part which is used for drying the smear which is dyed and washed, and a control part which is used for controlling the transmission part, the liquid dripping part, the mixing part, the gland part and the drying part according to preset operation steps. The observation liquid prepared by using the system not only has a better observation effect, but also enables the observation to be more efficient.

The invention discloses a high-throughput detecting system for obtaining trace mass and molecular structure information. A micro cantilever array fixed on a piezoelectric ceramic actuator is driven bya voice coil motor to translate along a width direction of a micro cantilever beam, so that laser beams are projected one by one onto the micro cantilever array, the laser reflected by a tip is detected by a photoelectric position sensitive detector to obtain a bending signal of a free end of each micro cantilever beam, and the vibration frequency of the micro cantilever beam is adjusted by the piezoelectric ceramic actuator; a micro Raman spectrum unit is disposed and is composed of an observation optical path sub-unit and a Raman laser optical path sub-unit, which work in a time division manner; the microstructure of a sample on the surface of the free end of the micro cantilever beam is obtained by using the observation optical path sub-unit; and a Raman spectrum is obtained by using the Raman laser optical path sub-unit, and then the molecular structure information of the sample is obtained. The high-throughput detecting system disclosed by the invention combines the micro cantilever beam sensing and micro Raman technology to realize the detection of trace mass changes, and is used for observing the appearance of the sample, and detecting the change of a molecular structure ina reaction process in real time.

The invention relates to a printing machine for multicolour printing, in which all printing units are configured in the same manner. Said machine is provided with a feed system for printable matter, which is suitable for both sheet-type and web-type printable matter. The printable matter can be connected to the feed system for printable matter in correct alignment and transported through all printing units (1-4) of the printing machine, using appropriate devices. The machine is provided with a transport belt, as the feed system for printable matter. Said belt is fed in a straight line through all the printing zones of the printing units (1-4) and is assigned to the printing cylinders (7). The transport belt can be attached to the printable matter by means of adhesion, contact pressure, application or using retaining strips.

The invention relates to the technical field of plant disease-resistance identification, and provides a method for inoculating sclerotinite to a rapeseed field stem. The method includes the followingsteps: collecting a centrifuge tube cover, preparing a mycelia module, field inoculating, and phenotypic observation statistics. The method utilizes the centrifuge tube cover to moisturize an agar block containing the mycelia, further moisturizes and fixes with a preservative film, and does not need to carry out artificial spraying for 7 days after the inoculation to maintain the field humidity topromote the disease. The method is simple and reliable, and is convenient for large-scale inoculation in the field; the inoculation efficiency is high, the success rate is high, and the result is stable; can effectively ensure the humidity and disease conditions of the mycelial block after field inoculation, and is suitable for large-scale inoculation identification of materials.