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Test strip for quickly detecting swine Japanese encephalitis antibody and preparation method of test strip

A technology for swine Japanese encephalitis and test strips, which is applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of being unsuitable for grass-roots use, being highly specialized, and taking a long time, achieving low production costs, avoiding virus spread, Simple and labor-saving effect

Inactive Publication Date: 2013-05-22
GUANGXI VETERINARY RES INST
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These detection methods have disadvantages such as time-consuming and highly professional, and special technicians and equipment are required for operation, so they are not suitable for grassroots use

Method used

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  • Test strip for quickly detecting swine Japanese encephalitis antibody and preparation method of test strip
  • Test strip for quickly detecting swine Japanese encephalitis antibody and preparation method of test strip
  • Test strip for quickly detecting swine Japanese encephalitis antibody and preparation method of test strip

Examples

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Embodiment 1

[0032] Example 1 Rapid detection of porcine Japanese encephalitis antibody test strips

[0033] 1 Preparation of JEV E protein antigen

[0034] 1.1 Main materials, reagents and instruments

[0035] Plasmid pET-32a-E and Escherichia coli BL21 (DE3) PlySs were prepared and preserved by the Virus Laboratory of Guangxi Veterinary Research Institute; IPTG was purchased from Promega Company of the United States; Ni-NTA protein purification resin was a product of QIAGEN Company; trisodium citrate (C 6 h 5 Na 3 o 7 2H 2 O), chloroauric acid (AuCl 3 HCl 4H 2O), bovine serum albumin (BSA) are all products of Sigma Company in the United States; nitrocellulose membrane (NC), glass fiber filter paper and water-absorbing material membranes are all products of Millipore Company; the test strip bottom plate is a product of Shanghai Pharmaceutical Industry Research Institute; BioJet XYZ3000 film spotting instrument and CM4000 test strip cutter are products of BioDot Company of the Unite...

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Abstract

The invention discloses a test strip for quickly detecting a swine Japanese encephalitis antibody and a preparation method of the test strip. The test strip consists of absorbent paper, a nitrocellulose membrane, a glass cellulose membrane, a sample pad and a support object, wherein the nitrocellulose membrane contains a detection line coated by JEV E protein and a control line coated by a goat anti-JEV antibody; and the glass cellulose membrane is combined with colloidal gold marked JEV E protein. According to the invention, by adopting a membrane chromatography indirect sandwich method, a specific antibody in Japanese encephalitis immune pig or infected pig serum can be detected. When detecting a swine Japanese encephalitis antibody, the test strip disclosed by the invention is simple, convenient and quick to operate, special instrument or professional training is not required, and the result is clear and easy to distinguish; and the test strip is easy to popularize and particularly suitable for base field test and epidemiological investigation, and plays an important auxiliary role in the diagnosis of swine Japanese encephalitis.

Description

technical field [0001] The invention belongs to the technical field of detecting porcine Japanese encephalitis, in particular to a test strip for rapid detection of porcine Japanese encephalitis antibody and a preparation method thereof. Background technique [0002] Japanese encephalitis B (Japanese encephalitis B, JE), also known as epidemic Japanese encephalitis, Japanese encephalitis, referred to as Japanese encephalitis, is a kind of disease caused by Japanese encephalitis virus (Japanese encephalitis virus, JEV). Zoonotic mosquito-borne natural foci of infectious diseases. The disease is very harmful to humans and is one of the most common vector-borne diseases of the human central nervous system, which can cause death of patients and often leave serious neurological sequelae. Among animals, pigs are the main source of infection of JE, which can amplify JEV, and JEV infection generally takes the form of a pig-mosquito-human chain. JE can cause miscarriage, stillbirth...

Claims

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Application Information

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IPC IPC(8): G01N33/569
Inventor 秦毅斌李斌赵武梁家幸何颖梁保忠苏乾莲闭炳芬陈忠伟段群棚卢冰霞
Owner GUANGXI VETERINARY RES INST
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