Molecular marker related to cucurbita pepo PRSV-W virus disease resistance and application of molecular marker

A technology of PRSV-W and molecular markers, which is applied in the determination/testing of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., and can solve the problem of no closely linked molecular markers for disease resistance genes

Active Publication Date: 2021-03-30
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at present, there are no research reports on molecular markers closely linked to the zucchini PRSV-W disease resistance gene that can be used at home and abroad.

Method used

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  • Molecular marker related to cucurbita pepo PRSV-W virus disease resistance and application of molecular marker
  • Molecular marker related to cucurbita pepo PRSV-W virus disease resistance and application of molecular marker
  • Molecular marker related to cucurbita pepo PRSV-W virus disease resistance and application of molecular marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0083] Embodiment 1, the identification of zucchini PRSV-W virus disease resistance character

[0084] In this example, primers used to identify or assist in the identification of PRSV-W virus disease resistance traits of zucchini are used to identify the PRSV-W virus disease resistance traits of zucchini by A1. A1 is composed of single-stranded DNA named P1 and P2, and P1 is The single-stranded DNA shown in positions 1-22 of SEQ ID No.1, and P2 is a single-stranded DNA reversely complementary to positions 162-144 of SEQ ID No.1.

[0085] 1. The zucchini to be identified are as follows, and 50 strains of each zucchini are selected for testing:

[0086] Xianyibai, a zucchini inbred line, is susceptible to PRSV-W virus disease;

[0087] Zucchini inbred line 08-13, whose phenotype is resistant to PRSV-W virus disease;

[0088] Ailu No. 2, whose phenotype is susceptible to PRSV-W virus disease, is a product of Beijing Aipu Green Agricultural Technology Co., Ltd.;

[0089] Yuhu ...

Embodiment 2

[0114] Example 2, the acquisition method of the SSR molecular marker closely linked with the dominant disease resistance gene CpPRSV-W of zucchini PRSV-W virus disease

[0115] The molecular marker of zucchini PRSV-W virus disease in Example 1 is named molecular marker Cp126, and this embodiment specifically describes the SSR molecular marker Cp126 closely linked with the dominant resistance gene CpPRSV-W of zucchini PRSV-W virus disease The method of obtaining includes the following steps:

[0116] (1) Zucchini inbred lines Xianyibai and 08-13F 2 Generation generation and phenotyping:

[0117] (1) The inbred line Xianyibai (female, P1) of zucchini was crossed with 08-13 (male, P2) to obtain the hybrid F 1 , F 1 F 2 generation groups.

[0118] (2)F 2 The single plant of the generation group was planted in a nutrient pot in a greenhouse, covered with an insect-proof net, and inoculated with PRSV-W virus disease after the cotyledons were fully expanded, and the disease res...

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Abstract

The invention relates to a molecular marker related to cucurbita pepo PRSVW virus disease resistance and application thereof. The cucurbita pepo PRSV-W virus disease molecular marker provided by the invention is a DNA molecule obtained by taking genome DNA of cucurbita pepo as a template and conducting amplifying by adopting a primer pair A1, wherein A1 is composed of single-stranded DNAs named asP1 and P2, P1 is single-stranded DNA specifically combined with the upstream of the 39th site of SEQ ID No. 1, and P2 is single-stranded DNA specifically combined with the downstream of the 54th siteof SEQ ID No. 1. Experiments prove that the resistance condition of the cucurbita pepo PRSV-W virus disease can be identified by utilizing the primer pair A1 for identifying or assisting in identifying the resistance character of the cucurbita pepo PRSV-W virus disease and the molecular marker of the cucurbita pepo PRSV-W virus disease.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a molecular marker related to the resistance of zucchini PRSV-W virus disease and its application. Background technique [0002] Zucchini (Cucurbita pepo L.) is an important melon vegetable in my country, and its cultivated area tends to expand year by year. Especially, the cultivated area in protected areas is second only to cucumber. It has become an important crop for increasing farmers' income and adjusting agricultural structure. [0003] Papaya ringspot virus watermelon strain (PRSV-W) is one of the main virus diseases that harm the production of zucchini. It is common and serious in the field. After the zucchini plants are infected with PRSV-W virus disease, the leaves show symptoms such as mosaic, shrinkage and curling. In the later stage, the plants will be dwarfed, the surface of the fruit will be bumpy and uneven, the flesh will be stiff and bitter, and the fruit will lose...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 张国裕李海真张帆田佳星贾长才
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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