Pea anti-powdery mildew er1 allele er1-7 and gene er1-7 linked-molecular marker

Abstract

The invention relates to a pea anti-powdery mildew er1 new allele er1-7 and a gene er1-7 linked-molecular marker, wherein the allele is positioned on an er1 gene locus of the VI linkage group of a pea genetic map. A pea resource G0003967 is immune to China powdery mildew isolate EPYN and EPBJ. Through a genetical analysis of resistance, genetic linkage mapping and determining on a candidate gene PsMLO1cDNA sequence of er1, an anti-disease gene of G0003967 is authenticated, and it is found that compared with a wild type susceptible gene PsMLO1cDNA sequence, the 111-120 base groups of an open reading frame of the PsMLO1cDNA sequence of the G0003967 are deleted, thus causing change of protein functions of PsMLO1. Such small fragment deletion mutation is a new PsMLO1 deviant form, and shows that the anti-powdery mildew gene contained in the G0003967 is a new er1 allele and is named as er1-7, thus providing a new gene resource for molecular breeding of powdery mildew resistance of pea resource.

Description

technical field [0001] The invention relates to the fields of plant pathology, genetic breeding and molecular biology, in particular to a new pea powdery mildew resistant er1 allele er1-7 and a molecular marker linked to the gene er1-7. Background technique [0002] Pea (Pisumsativum L.) is an important legume crop in the world. Pea powdery mildew caused by powdery mildew (Erysiphepisi D.C.) is a worldwide important disease, which generally occurs in temperate and subtropical regions, especially in warm daytime and cold nighttime climate conditions, causing serious damage, causing 25%~ 50% yield loss, and the loss of severely infected pea varieties can reach more than 80% (Nisare et al., 2011; Fondevilla et al., 2012). [0003] The most economical, effective and environmentally safe way to control pea powdery mildew is to plant disease-resistant varieties. At present, a large number of powdery mildew-resistant pea resources have been identified abroad, and three independen...

AI Technical Summary

Problems solved by technology

The resistance mechanism of er2 is to limit the development of powdery mildew through the death of host cells when pathogens invade, and only show resistance on leaves, and the resistance is affected by various factors such as temperature and leaf age, and in most cases it is expressed as susceptibility, which limits its application in breeding (Fondevilla et al., 2006)

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