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SSR (simple sequence repeat) molecular marker V for identifying descendant plants of Gala apple and application thereof

A molecular marker, Gala apple technology, applied in the field of plant genetic breeding and apple germplasm innovation research

Inactive Publication Date: 2017-05-31
AGRI BIOTECH RES CENT OF SHANXI PROVINCE +2
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0007] So far, there has been no report on the SSR molecular markers of Gala apple anther cultured plants

Method used

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  • SSR (simple sequence repeat) molecular marker V for identifying descendant plants of Gala apple and application thereof
  • SSR (simple sequence repeat) molecular marker V for identifying descendant plants of Gala apple and application thereof
  • SSR (simple sequence repeat) molecular marker V for identifying descendant plants of Gala apple and application thereof

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Embodiment Construction

[0025]Homozygous genotypes play an important role in the study of genetic mechanisms and breeding applications in higher plants (Murovec, et al. Haploids and doubled haploids in plant breeding. In: Abdurakhmonov I (ed) Plant Breeding: 2012: 87-106.). Apple is a fruit tree species with a highly heterozygous genome. Using a haploid genome can greatly reduce the difficulty of genome assembly (Dunwell. Haploids inflowering plants: origins and ex-ploitation. Plant Biotechnol J, 2010, 8 (4): 377-424. ). Apple is a perennial herb with a long reproductive cycle and self-incompatibility, which makes it difficult to obtain homozygous plants through multiple generations of selfing. The use of anther culture to induce embryoid bodies to obtain homozygous genotype lines is of great significance for apple breeding and genetic analysis of highly heterozygous genotypes (Germanà. Gametic embryogenesis and haploid technology as valuable support to plant breeding. Plant Cell Rep, 2011 , 30(5): ...

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Abstract

The invention belongs to the field of innovation and research of plant genetics breeding and apple germplasm, in particular to an SSR (simple sequence repeat) molecular marker V for identifying descendant plants of Gala apple and application thereof. The SSR molecular marker V is characterized in that in the detection process, the SSR molecular markers linked with No.9 and No.17 chromosomes are simultaneously used, DNA (deoxyribonucleic acid) of an apple gene group is used as a PCR (polymerase chain reaction) amplification template, the SSR molecular markers are respectively used as primer pairs, and the PCR amplification and product detection are performed, so as to identify the genetics linkage, anther culture plants and variety sources. The SSR molecular marker V has the advantages that the SSR molecular marker is used for identifying the apple linkage group of the identifying material for the first time, and the SSR molecular markers linked with No.9 and No.17 chromosomes of the apple are disclosed; the molecular marker is a co-dominant marker, so that the Gala apple genetic linkage group, the anther culture plant and the Gala source plant are quickly and accurately identified; the molecular level support is provided for further accelerating the utilization of the Gala apple and important agronomic trait linkage gene, and the genetic breeding of apple homozygous plants.

Description

technical field [0001] The invention belongs to the fields of plant genetic breeding and apple germplasm innovation research, and specifically relates to an SSR molecular marker V for identifying descendant plants of Gala apples and an application thereof. Background technique [0002] apple( Malus domestica Borkh.) has strong ecological adaptability and high nutritional value of fruit. It is one of the fruit tree species with wide cultivation area, large consumption and good economic benefits in the world. my country is one of the countries with the widest apple cultivation area and the highest total output. Especially in northern my country, apple is the fruit tree species with the largest cultivation area, and its output and area both rank first in the country's fruit. This industry has become a pillar industry in many provinces and cities in our country, and they play an increasingly important role in increasing farmers' income and promoting local economic development...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 侯丽媛曹秋芬邓舒张春芬肖蓉聂园军董艳辉李亚莉王亦学秦永军
Owner AGRI BIOTECH RES CENT OF SHANXI PROVINCE
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