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Linked molecular marker for dominant resistance gene ZYMV-2 of cucurbita pepo L. ZYMV and application of linked molecular marker

A technology of ZYMV-2 and molecular markers, which is applied in the application field of detection of disease-resistant plants, can solve the problems of lack of zucchini ZYMV disease-resistant genes, etc., and achieve the effects of convenient identification, stable amplification, and accelerated utilization

Inactive Publication Date: 2015-04-08
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But at present, there is no research report on molecular markers closely linked with ZYMV disease resistance gene of zucchini at home and abroad.

Method used

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  • Linked molecular marker for dominant resistance gene ZYMV-2 of cucurbita pepo L. ZYMV and application of linked molecular marker
  • Linked molecular marker for dominant resistance gene ZYMV-2 of cucurbita pepo L. ZYMV and application of linked molecular marker
  • Linked molecular marker for dominant resistance gene ZYMV-2 of cucurbita pepo L. ZYMV and application of linked molecular marker

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Experimental program
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Embodiment 1

[0035] ZYMV dominant disease resistance gene in zucchini ZYMV-2 Tightly linked SSR molecular marker, said molecular marker is ZY-126, which has the DNA sequence described in SEQ ID NO. 1 and SEQ ID NO. 2 in the sequence listing.

[0036] The ZYMV dominant disease resistance gene of zucchini ZYMV-2 The method for obtaining closely linked SSR molecular markers is characterized in that it comprises the following steps:

[0037] The SSR molecular markers were obtained by using the SSR-BSA technique using the following method: using zucchini inbred lines BS11 and BS6 as susceptible and resistant parents to prepare F 2 Generation segregation population, using 584 pairs of SSR primers to screen the ZYMV dominant resistance gene in zucchini ZYMV-2 Linked molecular markers, a co-dominant molecular marker, ZY126, and disease resistance gene ZYMV-2 The genetic linkage distance of 0.7 cM.

[0038] The application of the molecular marker in the identification and transfer of ZYMV d...

Embodiment 2

[0048] This embodiment specifically describes the ZYMV dominant disease resistance gene of zucchini and zucchini ZYMV-2 A method for obtaining closely linked SSR molecular markers, comprising the following steps:

[0049] (1) Zucchini inbred lines BS11 and BS6 F 2 Generation generation and phenotyping:

[0050] (1) Hybrid F obtained by crossing zucchini inbred line BS11 (female parent) and BS6 (male parent) 1 , F 1 F 2 generation groups.

[0051] (2)F 2 The single plant of the generation group was planted in a nutrient pot in a greenhouse, covered with an insect-proof net, and inoculated with viral diseases after the cotyledons were fully unfolded, and the disease resistance traits were investigated 15 days after the inoculation. The identification results are shown in Table 1.

[0052] Table 1 BS11×BS6 F after inoculation with ZYMV virus disease 2 Generational population genetic analysis

[0053]

[0054] R and S represent resistant and susceptible plants, respec...

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Abstract

The invention discloses an SSR molecular marker (ZY-126) linked with a dominant resistance gene ZYMV-2 of a zucchini yellow mosaic virus (ZYMV) of cucurbita pepo L., belongs to the technical field of breeding of vegetable molecules for disease resistance, and relates to an SSR molecular marker linked with the dominant resistance gene of ZYMV, and an application of the SSR molecular marker. The genetic linkage distance between the molecular marker ZY-126 and the resistance gene is 0.7cm. Whether a disease resistance character transfer offspring plant carries with the resistance gene ZYMV-2 or not can be rapidly and accurately detected by virtue of the molecular marker ZY-126, thus the disease-resistant breeding efficiency is significantly improved; the breeding cycle is shortened; and a solid foundation is laid for breeding of disease-resistant varieties of the ZYMV. The obtained molecular marker ZY-126 has the advantages of being stable, reliable, simple to operate, good in repeatability and the like, and has high utilization value in identification of resistant and susceptible varieties and a disease-resistant transfer single offspring plant.

Description

technical field [0001] The present invention relates to a kind of with zucchini ( Cucurbitapepo L.) Dominant resistance gene to zucchini yellow mosaic virus (ZYMV) ZYMV-2 Linked SSR molecular markers and the application of the markers in detecting disease-resistant plants. Background technique [0002] Zucchini is one of the most important vegetables widely grown in the world. The cultivated area in my country is increasing year by year. Especially in recent years, protected cultivation has developed rapidly, and it has become the second largest cultivated crop after cucumber. It is of great significance to increase farmers' income, adjust agricultural structure and enrich people's vegetable basket. [0003] Zucchini yellow mosaic virus (ZYMV) is one of the main diseases that endanger the production of Cucurbitaceae crops such as zucchini. The spread of ZYMV spreads rapidly all over the world. In recent years, the damage to zucchini production and seed production in my ...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
Inventor 张国裕李海真张帆贾长才姜立纲
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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