Bio-orthogonal activated time-resolved response type rare earth probe and its preparation method and application

A time-resolved, bio-orthogonal technology, applied in the direction of material excitation analysis, chemical instruments and methods, preparations for in vivo experiments, etc., can solve the problems of high copper ion toxicity, unfavorable large-scale promotion, large fluorescence background, etc., to achieve good water solubility
CN111732576BActive Publication Date: 2021-08-06SOUTH CHINA NORMAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SOUTH CHINA NORMAL UNIVERSITY
Publication Date
2021-08-06

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Abstract

The invention discloses a bioorthogonal activated time-resolved responsive rare-earth probe and a preparation method and application thereof, belonging to the technical field of biological markers and imaging. The rare earth probe provided by the present invention contains a tetrazine group, which is combined with Tb 3+ However, the rare earth ion Tb undergoes a bioorthogonal reaction with D-mannose cyclooctene, and the fluorescence resonance energy transfer in the probe cannot occur, resulting in the rare earth ion Tb 3+ The characteristic fluorescence is released, and the probe solution is accompanied by a naked-eye color change from pink to colorless, which can be used for cell membrane imaging of tumor cells; in addition, the rare earth probe has good water solubility and will be used in the solution to tumor cells. The problem of autofluorescence caused by imaging processes such as zebrafish and zebrafish is of great significance.
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Description

technical field

[0001] The invention belongs to the technical field of biological labeling and imaging, in particular to a bio-orthogonal reaction-based probe with time-resolved fluorescence responsiveness and a preparation method thereof, in particular to a bio-orthogonal activated time-resolved response type rare earth probe and a preparation method thereof and application. Background technique

[0002] Bioorthogonal reaction is a type of chemical reaction that can be carried out in living cells without interfering with the biochemical reaction of the organism itself, mainly including azide and alkynyl or phosphoric acid groups, tetrazine and alkenes or trans-cyclooctene , has the characteristics of high selectivity and rapid response. Therefore, the development of bioorthogonal reaction probes is very necessary in broadening biological applications.

[0003] At present, this reaction combined with organic fluorescent small molecule probes and its imaging technology has ...

Claims

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