Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Construction and screening of drosophila biological model

A biological model, Drosophila technology, applied in the biological field, can solve difficult problems such as the analysis of intermolecular interactions of histones

Inactive Publication Date: 2015-01-28
中国医科大学
View PDF2 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current research work can only be carried out on the basis of capturing the internal chromatin structure changes, so it is difficult to conduct an in-depth genetic analysis of the intermolecular interactions of histones

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Taking human androgen receptor (AR) as the representative of nuclear receptor, AR-mediated transcriptional regulation biological system was constructed in Drosophila. Because it is necessary to clarify the regulatory function of chromatin structure to study the mechanism of transcriptional regulation, the AR-mediated transcriptional regulation system constructed in this project is an in vivo system that uses both chromatin structure changes and transcriptional regulation as indicators. Puncta position effect (PEV) is an analysis system related to the state and transcription frequency of heterochromatin and euchromatin, which is often used in studies to analyze the regulation of chromatin structure. Under special conditions in Drosophila, the P sequence (P element) can be randomly inserted into the Drosophila chromosomal gene. The PEV analysis system uses this characteristic of Drosophila to insert the Drosophila red eye gene sequence (white) between the P sequence at th...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses construction and screening of a drosophila biological model. A system screening method comprises the following steps: S1, performing a first screening through observing a phenotypic change of drosophila eye colors decided by a red eye gene of the drosophila; S2, performing a second screening based on the first screening through detecting the strength change of a green fluorescent protein gene expressed on eye plates of drosophila larvae. By the construction and screening of the drosophila biology model disclosed by the invention, the drosophila biology model which can observe a chromatin structure change and can observe the mediated gene transcription regulation level change of a receptor is constructed.

Description

technical field [0001] The invention relates to the biological field, in particular to the construction and screening of a fruit fly biological model. Background technique [0002] At present, the research on the regulation of chromatin structure in organisms is mainly carried out with yeast or Drosophila molecular genetic analysis system. In this research field, researchers have conducted detailed studies on the new functions of known factors and their localization on chromosomes using an analysis system that includes intrinsic chromatin structural changes, and have been highly evaluated by world-class journals . In recent years, further studies have been conducted on tissue-specific histone modifying enzymes and histone replacement mechanisms. However, the current research work can only be carried out on the basis of capturing the internal chromatin structure changes, so it is difficult to conduct an in-depth analysis of the molecular interactions of histones genetically...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01K67/033G01N21/64
CPCA01K67/0339G01N21/6486
Inventor 赵越王春玉林琳邹仁龙王胜利孙洪邈孙士莹吴怡
Owner 中国医科大学
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com