Preparation method of fertilizer for preventing and controlling rice blast
A technology for rice blast and fertilizer, which is applied in the field of fertilizer preparation, can solve problems such as environmental pollution, pesticide residues, waste of human and material resources, etc., and achieve the effects of improving crop yield, improving cold resistance, and improving vitality and development.
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preparation example Construction
[0026]A preparation method for a fertilizer for preventing and treating rice blast, comprising the steps of:
[0027] (1) Take brassinolide at a mass ratio of 2:75~80:3, add an ethanol solution with a mass fraction of 80% and diethyl aminoethyl hexanoate for mixing, after dissolving, a mixture is obtained, and add it to the mixture at a mass ratio of 20:1 : 100, add nonylphenol polyoxyethylene ether and water, stir at 30-40°C for 2-3 hours to obtain a compound preparation, and set aside;
[0028] (2) Take kiwifruit stem and leaf powder and add phosphate buffer solution and cellulase at a mass ratio of 3:10:2, enzymolyze it at 30-40°C for 1-2 hours, inactivate the enzyme for 4-5 minutes, and take the inactivated substance at a mass ratio of 1 : 30~35 Add absolute ethanol and mix, under the condition of microwave power 300W, microwave once every 1min, each time for 1min, extraction time 5~6min, centrifuge at 6000r / min for 10min at 3~4℃, filter , take the filtrate and concentrat...
example 1
[0032] Vitamin B 100x: In terms of parts by mass, take 0.2 parts of thiamine hydrochloride, 0.3 parts of riboflavin, 0.03 parts of niacin, 0.5 parts of vitamin B6, 1 part of inositol, 0.5 parts of calcium pantothenate, and 3 parts of aminobenzoic acid , 4 parts of biotin, and 1000 parts of pure water were mixed and sterilized by filtration through a microporous membrane with a diameter of 0.22 μm.
[0033] Humic acid agar medium: in parts by mass, take 1 part of humic acid, 0.2 part of disodium hydrogen phosphate, 0.8 part of potassium chloride, 0.02 part of magnesium sulfate heptahydrate, 0.05 part of ferrous sulfate heptahydrate, 0.01 part of calcium carbonate , 18 parts of agar powder, 1 part of VitaminB 100x, 1000 parts of pure water, pH value 7.0, sterilized at 121°C for 20 minutes, cooled to 50°C, taking the cooled product and adding benomyl at a mass ratio of 1000:1 to obtain humic acid agar Medium.
[0034] Pretreatment of kiwifruit stems: Wash fresh kiwifruit stems a...
example 2
[0042] Vitamin B 100x: In parts by mass, take 0.5 parts of thiamine hydrochloride, 0.6 parts of riboflavin, 0.05 parts of niacin, 0.8 parts of vitamin B6, 2 parts of inositol, 1.2 parts of calcium pantothenate, and 5 parts of aminobenzoic acid , 6 parts of biotin and 1000 parts of pure water were mixed, and sterilized by filtration through a microporous membrane with a diameter of 0.22 μm.
[0043] Humic acid agar medium: in parts by mass, take 4 parts of humic acid, 0.4 part of disodium hydrogen phosphate, 1.2 part of potassium chloride, 0.05 part of magnesium sulfate heptahydrate, 0.09 part of ferrous sulfate heptahydrate, 0.04 part of calcium carbonate , 25 parts of agar powder, 3 parts of VitaminB 100x, 1000 parts of pure water, pH value 7.4, sterilized at 121°C for 25 minutes, cooled to 55°C, taking the cooled product and adding benomyl at a mass ratio of 1000:2 to obtain humic acid agar Medium.
[0044] Kiwifruit stem pretreatment: wash fresh kiwifruit stems and leaves,...
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