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Primers for molecular detection of nontoxic genes of Magnaporthe grisea and application thereof

A non-toxic gene and rice blast fungus technology, which is applied in the field of crop disease prevention and disease-resistant breeding, can solve the problems of large space, time-consuming manual inoculation, and isolation of germs, etc., to achieve wide application range, good practicability, and overcome laborious Effect

Inactive Publication Date: 2012-07-04
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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Problems solved by technology

The main drawback of this method is the need to isolate pathogens, and the manual inoculation takes a long time. It takes at least 30 days from seedling cultivation to the completion of plant phenotype investigation. In addition, rice plants need low temperature and moisture treatment after artificial inoculation. Inoculation assays, which need to be isolated from each other, thus requiring a larger space with controlled environmental conditions

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  • Primers for molecular detection of nontoxic genes of Magnaporthe grisea and application thereof
  • Primers for molecular detection of nontoxic genes of Magnaporthe grisea and application thereof
  • Primers for molecular detection of nontoxic genes of Magnaporthe grisea and application thereof

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Embodiment Construction

[0032] The present invention will be further described below in conjunction with specific implementation examples. It should be understood that the following examples are only used to illustrate the present invention and are not intended to limit the scope of protection of the present invention.

[0033] (1) Analysis of avirulence genes of blast fungus in different regions of Heilongjiang Province

[0034] Rice blast disease samples were collected from Jiansanjiang, Shangzhi, Suihua, Jiamusi Agricultural Science Institute and 856 farms in Heilongjiang Province in the autumn of 2009, and the single-spore isolated rice blast strains were used for the detection and analysis of Avrpik and Avrpizt avirulence genes.

[0035] Firstly, the strains of Magnaporthe grisea preserved on filter paper sheets were activated on the slant of tomato-oat medium, then subcultured on the plate of tomato-oat medium, cultured at 26°C for 5-7 days, and the bacteria on the surface of the medium were sc...

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Abstract

The invention relates to primers for molecular detection of nontoxic genes of Magnaporthe grisea and a use method thereof, is used for rapid molecular detection of nontoxic genes Avrpik and Avrpizt of Magnaporthe grisea, and belongs to the fields of crop disease control and breeding for disease resistance. According to the invention, two pairs of primers Avrpik-F / R and Avrpizt-F / R are designed, and used for respectively detecting the nontoxic genes Avrpik and Avrpizt of Magnaporthe grisea, wherein the lengths of the specifically amplified DNA (Deoxyribonucleic Acid) fragments are 184 bp and 153 bp respectively. The primers are suitable to be used for analyzing nontoxic gene composition of pure Magnaporthe grisea culture and Magnaporthe grisea colony from disease position.

Description

technical field [0001] The invention relates to a primer for the molecular detection of the avirulent gene of the rice blast fungus and a usage thereof, which is specially used for the rapid molecular detection of the rice blast fungus avirulent gene Avrpik and Avrpizt, and belongs to the field of crop disease prevention and disease-resistant breeding. Background technique [0002] Rice blast, caused by Magnaporthe oryzae (anamorphic: Pyricularia oryzae), is a sudden and epidemic disease of rice. Using host resistance, breeding and planting disease-resistant varieties can effectively control the occurrence of plant diseases. So far, through extensive genetic analysis, 85 rice blast resistance genes and 347 quantitative trait loci (Quantitative trait loci, QTL) have been located, and most of the genes and QTL are located on different chromosomes of rice, among which 11 of pi21, Pi21, Pi22, Pi23, Pi34, Pi35(t), Pb1, Pif, Pikur1, Pikur2, and Pi-sel belonged to partial resistan...

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11C12R1/645
Inventor 周永力李祥晓徐建龙王疏黎志康
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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