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Rice induced disease-resistant gene OsAAA1 and application thereof

A technology for inducing disease resistance and genes, applied in the field of genetic engineering

Inactive Publication Date: 2015-03-04
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the involvement of AAA-ATPase gene in rice disease resistance

Method used

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  • Rice induced disease-resistant gene OsAAA1 and application thereof
  • Rice induced disease-resistant gene OsAAA1 and application thereof
  • Rice induced disease-resistant gene OsAAA1 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027]Example 1 OsAAA-ATPase-1 gene is induced to up-regulate expression by Magnaporthe grisea

[0028] At the 4-5 leaf stage of the rice line Nipponbare seedlings, use the rice blast strains race007 and race102 that are compatible with Nipponbare (Compatible) and Incompatible (Incompatible) to inoculate, and set up Mock controls at the same time, and collect 2 and 3 samples before and after inoculation respectively. , 4, 5 and 6 d in the young leaves at different time points, and the expression changes of OsAAA1 were analyzed by qRT-PCR technology. see results figure 1 ,Depend on figure 1 It can be seen that the expression of this gene is strongly induced by Magnaporthe grisea.

Embodiment 2

[0029] Example 2 OsAAA1 gene depends on SA-induced up-regulated expression

[0030] At the 3-4 leaf stage of Nipponbare seedlings, the roots were treated with phytohormones ABA, ACC, BTH (functional analog of SA), CK, IAA, JA and GA at a certain concentration respectively, and a Mock control was set up, and the samples were collected after 24 h of treatment. In the leaves, the response of OsAAA-ATPase-1 was analyzed by qRT-PCR. see results figure 2 , the results showed that the gene was induced by salicylic acid. In order to further prove the response of the gene to SA, the stably inherited salicylic acid decomposing enzyme NahG transgenic lines NahG#3 and NahG#6 were used to inoculate the rice blast strain race007, and a Mock control was set at the same time, and 2 samples were collected before and after inoculation. and young leaves at different time points of 4 d for expression analysis, the results are shown in figure 2 It showed that in SA-deficient plants, the gene ...

Embodiment 3

[0031] Example 3 Overexpression of the OsAAA1 gene shows obvious resistance to rice blast and bacterial blight

[0032] The OsAAA1 full-length gene overexpression vector was constructed, and the Agrobacterium-mediated genetic transformation was used to transform into the Nipponbare recipient strain. The expression analysis of the T0 and T1 generations was carried out, and the strains with up-regulated expression were inoculated with compatible rice blast fungus and blight Leaf blight bacteria, all showed obvious disease resistance, and the results were as follows: image 3 and Figure 4 shown.

[0033]

[0034]

[0035]

[0036]

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PUM

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Abstract

The invention provides a rice induced disease-resistant gene OsAAA1 which is doubly induced by salicylic acid and magnaporthe grisea. The nucleotide sequence of the rice induced disease-resistant gene OsAAA1 is as shown in SEQ ID NO:1. The amino acid sequence of a rice disease-resistant protein coded by the rice induced disease-resistant gene OsAAA1 is as shown in SEQ ID NO:2. The rice induced disease-resistant gene OsAAA1 provided by the invention can be applied to breeding of a rice variety with resistance to rice blast and bacterial blight. A specific application mode is as follows: an excessive expression vector is constructed according to the rice induced disease-resistant gene OsAAA1 provided by the invention; the excessive expression vector is transferred into a receptor variety by utilizing agrobacterium-mediated genetic transformation. The variety shows the antibacterial property for magnaporthe grisea and xanthomonas oryzae under the induction of salicylic acid.

Description

technical field [0001] The invention provides a rice induced disease resistance gene OsAAA1 which is double-induced by salicylic acid and rice blast fungus. The gene is used for rice blast and bacterial blight molecular breeding and belongs to the technical field of genetic engineering. Background technique [0002] Rice is one of the world's important food crops, animal feed and bioenergy. Rice blast, bacterial blight and sheath blight are the three most serious diseases in rice production. From the point of view of environmental protection and agricultural sustainable development, cultivating and rationally using disease-resistant strains is the most economical, effective and environmentally friendly means to control these diseases. However, the resistance mediated by the main disease resistance gene is often due to the diversity and variability of the pathogen population, resulting in the loss of resistance after 3-5 years of popularization of disease-resistant strains. ...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/82C07K14/415A01H5/00
Inventor 刘新琼姜昌杰王春台程钢刘学群
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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