Method for biosynthesis of cyclic lipopeptide surfactant

Abstract

The invention discloses a method for biosynthesis of acyclic lipopeptide surfactant. The method comprises the steps of first taking seawater with additional ammonium chloride as a culture medium, and performing staphylococcus WX2701 low-temperature anaerobic fermentation; performing sterile filtration on obtained fermentation broth, and sequentially adding achromobacter WX1501 and long-chain aliphatic hydrocarbon (C20-40) monomers or mixture into obtained filtrate to perform fermentation; and obtaining the cyclic lipopeptide surfactant having high surface activity through separation from the fermentation broth. According to the method, two-step microorganism total synthesis of the lipopeptide surfactant is adopted, mutual competition between microorganism strains is effectively avoided, a metabolite inhibiting effect is greatly reduced, the yield of the lipopeptide surfactant reaches 38.7 g / L, the yield of the surfactant produced by single bacterium fermentation is greatly improved, and the industrialization of the lipopeptide surfactant is expected to be achieved.

Description

technical field [0001] The present invention relates to a biosynthesis method, in particular to a biosynthesis method of a cyclolipopeptide surfactant. Background technique [0002] The existing microbial-derived cyclolipopeptide surfactants are mainly derived from the fermentation production of a single microbial strain. Although the yield of lipopeptide surfactants can be improved by optimizing the fermentation conditions such as nitrogen sources, due to the many synthesis steps of lipopeptide surfactants in microorganisms and strict regulation, the fermentation culture conditions of a single strain are required to be high and the yield is low ( ≤10g / L), which greatly increases the production cost, it is difficult to replace chemical surfactants in terms of price, and the large-scale promotion and application are limited. SUMMARY OF THE INVENTION [0003] In order to solve the above technical problems, the present invention discloses a biosynthesis method of a cyclolipo...

AI Technical Summary

Problems solved by technology

Although the production of lipopeptide surfactants can be improved by optimizing fermentation conditions such as nitrogen sources, lipopeptide surfactants have many synthesis steps in microorganisms and are strictly regulated, resulting in high requirements for single-strain fermentation and culture conditions and low yields ( ≤10g / L), which greatly increases the production cost, it is difficult to replace chemical surfactants in price, and the large-scale promotion and application are limited