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Method of removing lipopeptides from solutions and changing their structure

A technology of lipopeptide and solution, applied in the field of changing the structure of lipopeptide, can solve the problems of no environmental protection

Pending Publication Date: 2021-01-22
INVENTIONBIO SPOLKA Z OGRANIKZONA ODPOVIEDZIALNOSTSIYA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Basic chemical hydrolysis of lactone bonds resulted in 97% efficiency of the final product compared to only 56% efficiency of acid hydrolysis
However, chemical hydrolysis requires the use of toxic (methanol) and corrosive (NaOH, NH 3 h 2 0 or HCl) compounds, which makes the process less environmentally friendly

Method used

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  • Method of removing lipopeptides from solutions and changing their structure
  • Method of removing lipopeptides from solutions and changing their structure
  • Method of removing lipopeptides from solutions and changing their structure

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Surfactin is released from a post-fermentation solution containing a mixture of lipopeptides with a predominant surfactin content using known methods. 100ml of the resulting 0.8mg / ml surfactin aqueous solution was placed in a 250ml Erlenmeyer flask. Then add 1 gram of activated carbon, the particle size of activated carbon is 0.2 to 1mm, and the pore area is 650m 2 / g, and the pH of its aqueous suspension is 8.5. The suspension was mixed using a shaker at 20°C for 72 hours. After 72 hours, the solution was separated from the charcoal by filtration and centrifugation. The amount of adsorbed surfactin retained on the activated carbon was 5% of that originally introduced into the solution. The remainder of surfactin reacts on the surface of the activated carbon to form surfactin hydrolyzate having a linear structure represented by formulas (I) and (II).

[0025]

[0026] Figure 1. The amino acid sequence is the formula of the linear analogue of surfactin of L-Glu-L-Le...

Embodiment 2

[0028] Same as Example 1, the only difference is that the concentration of surfactin aqueous solution is 2 mg / ml. Subsequently, 3 g of activated carbon was introduced with a particle size of 0.1 to 0.2 mm and an internal surface area of ​​750 m 2 / g, pH 9 in aqueous suspension. The suspension was mixed using a shaker at 25°C for 72 hours. After 72 hours, the solution was separated from the charcoal by filtration and centrifugation. Surfactin was adsorbed on activated carbon in an amount of 3% initially introduced into the solution. The remainder of the surfactin reacts on the carbon surface to form a linear structured surfactin hydrolyzate. The mixture of linear products was separated into analogs with different alkyl chain lengths by preparative liquid chromatography, the structure of which is shown in formula (III).

[0029]

[0030] R 1 - Alkyl chains with 9 to 12 carbon atoms

[0031] Fig. 2 amino acid sequence is the formula of the linear analogue of surfactin of...

Embodiment 3

[0033] Same as Example 1, except that the surfactin solution was applied to the column. In aqueous suspension, the column is filled with 20 grams of activated carbon in the form of a monolith with a mesh size of 0.5 x 0.5 mm, a wall thickness of 0.5 mm and a pore area of ​​750 m 2 / g, pH value is 9. At 25° C., 2000 ml / h of 2 mg / ml surfactin in water was introduced from above using a peristaltic pump. After passing through the activated carbon bed, the solution is directed to a treatment tank from which it is drawn using a pump for treatment in a flow system. This treatment was carried out for 48 hours. Surfactin was adsorbed on activated carbon in an amount of 10% initially introduced into the solution. The remaining part of surfactin reacted on the surface of activated carbon to form a linear structure of surfactin hydrolyzate, as shown in formulas (IV)–(VI), which remained in the circulating solution. Charcoal can be used multiple times in this process. Charcoal can be ...

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Abstract

The method for removing lipopeptides from solutions and changing their structure from cyclic to linear, characterised in that lipopeptide capture and sequestration takes place from solutions containing water through sorption, and in the case of cyclic lipopeptides, in which the peptide ring is closed by a lactone bond, sorption is additionally associated with hydrolysis reactions occurring on thesurface of active carbon, leading to linearisation as a result of breaking the lactone bond.

Description

technical field [0001] The subject of the present invention is a method for removing lipopeptides in solution and for modifying the structure of lipopeptides. Background technique [0002] Biosurfactants are surfactants produced by microorganisms or obtained by biotransformation. Compared with their synthetic analogues, biosurfactants are biodegradable, have low toxicity, and are highly resistant to extreme environmental conditions, while being outstanding in reducing the surface tension of phase boundaries. [0003] Lipopeptides are a group of biosurfactants whose molecules consist of cyclic peptides and β-hydroxy fatty acid chains linked by ester (lactone) bonds. The best known lipopeptide is surfactin, produced by various strains of Bacillus. The form of the lipopeptide can be a series of homologues which differ in the length of the carbon chain, 12-17 carbon atoms, as described by P.Biniarzand M.Lukaszewicz in "Direct quantification of lipopeptidebiosurfactants in bio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06
CPCC07K7/06C07K1/12C07K1/16C07K1/14B01D15/08
Inventor 马鲁克·库拉温斯基马辛·卢卡泽维奇汉娜·法蒂诺维奇
Owner INVENTIONBIO SPOLKA Z OGRANIKZONA ODPOVIEDZIALNOSTSIYA
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