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Aspartate amino transferase detection kit

An aspartate amino group, detection kit technology, which is applied in measurement devices, color/spectral property measurement, material analysis by optical means, etc., can solve the problems of low accuracy, cumbersome operation, unstable reagents, etc. Improve stability, improve accuracy, and eliminate the effect of interference

Inactive Publication Date: 2017-05-10
王贤俊
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] The purpose of the present invention is to provide an aspartate aminotransferase detection kit for clinical application, which mainly solves the problems of low accuracy, cumbersome operation, polluted environment, and unstable reagents in the existing AST detection field.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] The Aspartate Aminotransferase Detection Kit consists of Reagent 1 and Reagent 2. The specific components and contents are as follows:

[0047] Composition of Reagent 1:

[0048] Tris-HCl buffer 120mmol / L, PH=8.2

[0049] L-Aspartic Acid 400mmol / L

[0050] NADH0.2mmol / L

[0051] Malate dehydrogenase 800U / L

[0052] KCl 5g / L

[0053] Tween-20 0.5%

[0054] BSA 1%

[0055] Sucrose 5g / L

[0056] Proclin-300 0.5%

[0057] Composition of Reagent 2:

[0058] Tris-HCl buffer 100mmol / L, PH=7.4

[0059] α-ketoglutarate 60mmol / L

[0060] KCl 6g / L

[0061] PEG 6000 0.5%

[0062] Proclin-300 0.5%

Embodiment 2

[0064] The Aspartate Aminotransferase Detection Kit consists of Reagent 1 and Reagent 2. The specific components and contents are as follows:

[0065] Composition of Reagent 1:

[0066] Tris-HCl buffer 150mmol / L, PH=8.2

[0067] L-Aspartic Acid 500mmol / L

[0068] NADH0.3mmol / L

[0069] Malate dehydrogenase 1000U / L

[0070] KCl 8g / L

[0071] Triton X-100 1%

[0072] Glycerin 10%

[0073] Maltose 6g / L

[0074] Proclin-300 1%

[0075] Composition of Reagent 2:

[0076] Tris-HCl buffer 80mmol / L, PH=7.4

[0077] α-ketoglutarate 70mmol / L

[0078] KCl 8g / L

[0079] PEG 8000 1%

[0080] Proclin-300 1%

Embodiment 3

[0082] The Aspartate Aminotransferase Detection Kit consists of Reagent 1 and Reagent 2. The specific components and contents are as follows:

[0083] Composition of Reagent 1:

[0084] Tris-HCl buffer 180mmol / L, PH=8.2

[0085] L-Aspartic Acid 600mmol / L

[0086] NADH0.4mmol / L

[0087] Malate dehydrogenase 1200U / L

[0088] KCl 10g / L

[0089] Triton X-100 1.5%

[0090] PEG 8000 1.5%

[0091] Glycerin 15%

[0092] Fructose 6g / L

[0093] Glucose 6g / L

[0094] NaN 3 1g / L

[0095] Composition of Reagent 2:

[0096] Tris-HCl buffer 60mmol / L, PH=7.4

[0097] α-ketoglutarate 80mmol / L

[0098] KCl 10g / L

[0099] Tween-80 1%

[0100] PEG 6000 1%

[0101] NaN 3 0.6g / L

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Abstract

The invention relates to an aspartate amino transferase (AST) detection kit and belongs to the technical field of clinic in vitro diagnostic reagents. The invention aims to provide the aspartate amino transferase detection kit with capability of effectively eliminating endogenous alpha-oxoglutarate interference and high stability in order to achieve more accurate and reliable determination results of aspartate amino transferase. The kit provided by the invention comprises a reagent 1 and a reagent 2, wherein the reagent 1 comprises the following components: Tris-HCl buffer solution, L-asparaginic acid, reduction coenzyme (NADH), malic dehydrogenase (MDH), enzyme protective agent, stabilizer and preservative, and the reagent 2 comprises the following components: Tris-HCl buffer solution, alpha-oxoglutarate, a stabilizer and a preservative. The enzyme protective agent and the stabilizer are added into the AST detection kit provided by the invention so that the stability of the aspartate amino transferase detection kit is promoted; the aspartate amino transferase detection kit is suitable for various full-automatic biochemical analyzers; the operation is simple and safe; the aspartate amino transferase detection kit has convenience in clinical application and popularization.

Description

[0001] Technical field: [0002] The invention belongs to the technical field of clinical in vitro diagnostic reagents, and relates to an aspartate aminotransferase detection kit. [0003] Background technique: [0004] Aspartate aminotransferase (AST), also known as aspartate aminotransferase, mainly exists in cardiomyocytes, followed by liver cells. It is abundant in myocardial cells. When myocardial infarction occurs, the activity of AST in serum increases significantly within 6-12 hours after onset, reaches the peak at 48 hours, and returns to normal in about 3-5 days. Serum AST can also originate from liver cells. Various liver diseases can cause the increase of serum AST, and toxic hepatitis can even be higher. Muscle inflammation, pleurisy, nephritis, etc. can also cause a slight increase in serum AST, and there may also be damage to cardiomyocytes, liver cells, and skeletal muscle. Clinically, it is mainly used for auxiliary diagnosis of viral hepatitis, obstructive j...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N21/17
CPCG01N21/31G01N21/17
Inventor 王贤俊张敏
Owner 王贤俊
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