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Method for in-vitro directional differentiation inducing of mesenchymal stem cell towards melanocyte

A technology of mesenchymal stem cells and directed differentiation, applied in the direction of artificial cell constructs, animal cells, vertebrate cells, etc., can solve the problems of low immunogenicity and difficult acquisition, and achieve low immunogenicity, easy acquisition, and difficulty in individual differences Effect

Inactive Publication Date: 2014-12-24
AFFILIATED HOSPITAL OF JIANGSU UNIV
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  • Abstract
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  • Application Information

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Problems solved by technology

Objective disadvantages of existing technologies: sources of ES cells and sources of bone marrow or adipose-derived mesenchymal stem cells are limited or difficult to obtain, human umbilical cord mesenchymal stem cells (hUC-MSCs) are primitive and self Stronger ability to update and proliferate; low immunogenicity, will not trigger immune response; easy to obtain materials, high purity and no virus and tumor cell contamination, in view of the incomparable advantages of these other sources of mesenchymal stem cells, umbilical cord mesenchymal stem cells Mesenchymal stem cells have broad application prospects in scientific research and clinical practice
However, there is no report in the literature that hUC-MSCs can be induced to differentiate into melanocytes

Method used

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  • Method for in-vitro directional differentiation inducing of mesenchymal stem cell towards melanocyte
  • Method for in-vitro directional differentiation inducing of mesenchymal stem cell towards melanocyte
  • Method for in-vitro directional differentiation inducing of mesenchymal stem cell towards melanocyte

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Embodiment Construction

[0041] The present invention will be further described below in conjunction with specific implementation examples.

[0042] The method for inducing directional differentiation of umbilical cord-derived mesenchymal stem cells to melanocytes in vitro is completed according to the following steps:

[0043] (1) Cultivation of primary hUC-MSCs by explant method (subculture and morphological observation)

[0044] A. After the human umbilical cord is obtained from the operating table, it is immersed in 0.9% sterile saline and transported aseptically to the laboratory.

[0045] laboratory.

[0046] B. Take it out in the ultra-clean bench, wash 3 times with PBS buffer containing 1% double antibody (penicillin and streptomycin),

[0047] To remove blood stains and fully clean the lumen of the umbilical vein, peel off the arteries and veins, take the connective tissue of the umbilical cord, and cut it into 4-5mm3 tissue pieces.

[0048] C. Inoculate the tissue block into a 6-well ce...

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Abstract

The invention relates to a method for in-vitro directional differentiation inducing of a mesenchymal stem cell towards melanocyte, particularly relates to a method for in-vitro directional differentiation inducing of the mesenchymal stem cell with an umbilical cord source towards the melanocyte, and belongs to the technical field of biology. The mesenchymal stem cell with the umbilical cord source is cultivated by adopting an explants method, and then the mesenchymal stem cell with the umbilical cord source is identified, so as to determine the characteristics of the mesenchymal stem cell; a culture medium is induced to carry out in-vitro inducing differentiation to form the melanocyte by using improved melanine; and finally the cultivated melanocyte is identified. An ES cell source and a marrow or adipose tissue-derived stromal cell source are limited or not easy to obtain while the stem cell can be easily obtained from the umbilical cord, the immunogenicity is very low, and an individual difference is not easily generated, so that the method is suitable for large-scale clinical application, and the induced melanocyte can be applied to epichrosis leucasmus therapy.

Description

technical field [0001] The invention relates to a method for inducing directional differentiation of mesenchymal stem cells to melanocytes in vitro, in particular to a method for inducing directional differentiation of umbilical cord-derived mesenchymal stem cells to melanocytes in vitro, and belongs to the field of biotechnology. Background technique [0002] Vitiligo (vitiligo) is a common local or systemic depigmentation skin disease, the incidence of the disease is about 0.5-1%, and it is related to genetic factors, immune factors, oxidative stress imbalance, etc. It is the destruction of melanocytes caused by a combination of factors, which causes depigmentation and the formation of white spots. There are many treatment methods for this disease, such as drugs, laser, surgery, etc., but the curative effects are not exact, and some even have isomorphic reactions. [0003] In recent years, the method of stem cell transplantation to treat diseases has received more and mor...

Claims

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Application Information

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IPC IPC(8): C12N5/0775C12N5/071A61K35/12A61P17/00
CPCY02A50/30
Inventor 李遇梅李小战李兴天刘莉萍
Owner AFFILIATED HOSPITAL OF JIANGSU UNIV
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