A kind of method for extracting the pigment in the leaf of black rice plant

A technology of black rice leaves and pigments, which can be used in pharmaceutical formulations, non-central analgesics, medical preparations containing active ingredients, etc. rate, the effect of increasing dissolution

Inactive Publication Date: 2011-12-14
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, water or organic solvents are mostly used in the extraction of pigments from the leaves of the black rice tree in China, and the supercritical carbon dioxide fluid extraction method is also used to study the leaves of the black rice tree. However, the cost and equipment requirements of the supercritical carbon dioxide fluid extraction method are high, and the feasibility is limited.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Crush the dried black rice leaves, weigh 500g, add 12 times the amount of 5% acetic acid solution to soak for 4 hours, extract 3 times, filter with an 80-mesh filter to obtain the filtrate, perform high-speed centrifugation at 2900rpm, add the supernatant In the membrane separation system, ultrafiltration, the ultrafiltration membrane molecular weight cut-off is 3000-10000, nanofiltration, the nanofiltration membrane molecular weight cut-off is 200-300, the concentrated solution is obtained, dispersed with water, adsorbed on a polyamide column, first washed with water to remove impurities, and then Elute with 7BV50% ethanol solution, concentrate the eluate, and freeze-dry to obtain 23g of black rice leaf pigment.

Embodiment 2

[0018] Grind the dried black rice leaves, weigh 1kg, add 18 times the amount of 5% acetic acid solution, soak for 3 hours and extract 3 times, filter with a 160-mesh filter to obtain the filtrate, perform high-speed centrifugation at 2500rpm, and add the supernatant to the membrane In the separation system, ultrafiltration, the ultrafiltration membrane molecular weight cut-off is 3000-10000, nanofiltration, the nanofiltration membrane molecular weight cut-off is 200-300, and the concentrated solution is obtained, dispersed with water, adsorbed on a polyamide column, washed with water to remove impurities, and then used 5BV was eluted with 50% ethanol solution, the eluate was concentrated, and freeze-dried to obtain 56 g of the leaf pigment of U.

Embodiment 3

[0020] Crush the dried black rice leaves, weigh 1kg, add 15 times the amount of 5% acetic acid solution, soak for 2 hours and extract twice, filter with a 160-mesh filter to obtain the filtrate, perform high-speed centrifugation at 2500rpm, and add the supernatant to the membrane In the separation system, ultrafiltration, the ultrafiltration membrane molecular weight cut-off is 3000-10000, nanofiltration, the nanofiltration membrane molecular weight cut-off is 200-300, and the concentrated solution is obtained, dispersed with water, adsorbed on a polyamide column, washed with water to remove impurities, and then used 6BV was eluted with 50% ethanol solution, the eluate was concentrated, and freeze-dried to obtain 68 g of the leaf pigment of U.

[0021] Example 3:

[0022] Grind the dried black rice leaves, weigh 5kg, add 16 times the amount of 5% acetic acid solution, soak for 3 hours and extract 3 times, filter with a 100-mesh filter to obtain the filtrate, perform high-speed...

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PUM

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Abstract

The invention relates to a method for extracting pigments from the leaves of black rice leaves, comprising the following steps: (1) crushing the dried black rice leaves, adding 2-5 times the amount of 5% acetic acid solution to soak for 2-4 hours, and extracting 2-4 times , filter with a 80-160 mesh filter to obtain the filtrate; (2) The filtrate is subjected to high-speed centrifugation at 2500-2900rpm to obtain the supernatant; (3) The supernatant is added to the membrane separation system for ultrafiltration and nanofiltration to obtain The concentrated solution is dispersed with water, adsorbed on a polyamide column, washed with water to remove impurities, and then eluted with 5-7BV50% ethanol solution, concentrated eluent, and freeze-dried to obtain the leaf pigment of U. The leaf pigment extracted by the invention has high stability and high purity, less energy consumption and less pollution in the production process, and is suitable for industrialized production.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for extracting pigments in leaves of black rice plant. Background technique [0002] Black rice tree ( Vaccinium bracteatum Thunb . ) is a plant of the Rhododendronaceae Vaccinium genus, also known as Nanzhu, Wufanzi, etc. The active ingredients in the branches and leaves of Wufan tree include anthocyanins, flavonoids, tannins, triterpenes, etc., among which the content of water-soluble blue melanin is high, which can promote the resynthesis of rhodopsin, Vp-like function, and improve blood microcirculation , anti-ulcer, anti-inflammatory, anti-aging and other physiological activities. [0003] It has been reported in the literature (Hu Zhijie et al., Re-study on the extraction process and properties of the pigment of the leaves of Wufan tree [J]. China Wild Plant Resources, 2001, 22 (4): 47-48) The pigment extract of the leaves of Wufan tree is black paste, w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/45A61P9/00A61P17/02A61P29/00A61P39/06A61K127/00
Inventor 刘东锋万冬梅
Owner NANJING ZELANG MEDICAL TECH
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