Vaccinium australe tissue culture and rapid propagation method

Abstract

The invention discloses a vaccinium australe tissue culture and rapid propagation method. Vaccinium australe has the advantages of strong stress resistance, high growth speed and high yield. Therefore, establishment of a vaccinium australe in-vitro regeneration system is very necessary so as to lay a foundation for the cultivation of vaccinium australe with high quality, high yield and strong adaptability. According to the vaccinium australe tissue culture and rapid propagation method disclosed by the invention, stems with buds of vaccinium australe are taken as explants, a vaccinium australe in-vitro regeneration plant can be prepared by virtue of the steps such as induction culture, multiplication culture, rooting culture as well as acclimatization and transplanting, and thus the tissue culture and rapid propagation of vaccinium australe can be achieved so as to ensure that a new fast and effective way can be provided for mass propagation of vaccinium australe.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a method for tissue culture and rapid propagation of Nangao Congyue. Background technique [0002] Vaccinium belongs to Rhododendronaceae Vaccinium perennial deciduous or evergreen shrubs, the fruit is blue or red, native to North America. The fruit is blue or red, the size of the fruit varies with different types, the flesh is soft and juicy, the flavor is mellow, rich in vitamins and other special nutrients that are rarely found in fruits, and has high health care and medicinal value, so the international FAO lists it as one of the five health foods for human beings. my country's Vaccinium plant resources are very rich, but the concentrated cultivation area is still small, mainly concentrated in Daxinganling, Xiaoxing'an Mountains and Changbai Mountains. The propagation methods of lingonberry seedlings vary with varieties. High-bush bilberry ...

AI Technical Summary

Problems solved by technology

The propagation methods of lingonberry seedlings vary with varieties. High-bush bilberry mainly uses hard branch cuttings, rabbit-eye lingonberry adopts green branch cuttings, low-bush lingonberry hard branch cuttings and green branch cuttings can be used, other methods such as seedlings, Rhizome cuttings and ramets are also used, but these propagation methods have problems such as long cycle, low propagation coefficient, and high cost, which limit the work of rapid propagation and selection of new varieties.