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Vaccinium spp. anthocyanin and preparation method and application thereof

A blueberry anthocyanin and rabbit-eye blueberry technology, applied in organic chemistry, metabolic diseases, drug combination, etc., can solve the problems of anthocyanin structure damage, not suitable for mass production, large solvent consumption, etc., and achieve large desorption capacity , Excellent resolubility and low price

Inactive Publication Date: 2019-04-02
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the extraction of blueberry anthocyanins mainly adopts the heat reflux extraction method, which is not only inefficient and time-consuming, but also has low extraction yield, large solvent consumption, and high heating temperature during extraction, which will cause heat-sensitive components A large amount of degradation of anthocyanins; new technologies such as microwave-assisted extraction can effectively improve the extraction efficiency and purity of blueberry anthocyanins, but the structure of anthocyanins will also be destroyed due to high local temperature, and these technologies are costly and unsatisfactory. Suitable for industrial mass production

Method used

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  • Vaccinium spp. anthocyanin and preparation method and application thereof
  • Vaccinium spp. anthocyanin and preparation method and application thereof
  • Vaccinium spp. anthocyanin and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Take by weighing 2500g rabbit eye blueberry, after putting into blender and crushing, add 10L volume concentration and be 95% acid ethanol solution (hydrochloric acid concentration 0.02%, V / V) and stir evenly, leaching under normal temperature condition 24 hours, this extraction process repeats 2 The second time, the two extracts were combined, then vacuum filtered and the filtrate was collected, and concentrated in vacuum at 45°C to 500mL, and the obtained purple-red concentrated solution was the crude blueberry anthocyanin extract.

[0036] Fill the pretreated AB-8 macroporous resin into the chromatographic column, fill the column with distilled water, the liquid level is 1cm higher than the filler, and use the countercurrent perfusion method to remove the air bubbles in the chromatographic column. Use a plastic dropper to draw 500mL of the above-mentioned blueberry anthocyanin crude extract, the loading volume is 0.5 times the volume of the resin column, slowly add it...

Embodiment 2

[0038] After washing and homogenizing 500g of rabbit-eye blueberry frozen fruit, add 5L of 80% acidic ethanol solution (containing 0.1% hydrochloric acid, V / V), and carry out ultrasonic-assisted extraction in a water bath at 30°C, and extract once , the extraction time is 40 minutes. The extract was filtered through a 200-mesh filter cloth, and the filtrate was concentrated in vacuum to 250 mL to obtain a crude blueberry anthocyanin extract.

[0039] Put the crude extract of blueberry anthocyanin on a 500mm×50mm macroporous resin column, the resin model is AB-8, and the sample volume of the crude extract of blueberry anthocyanin is 0.1 times the volume of the resin column. After loading, the adsorption time is 12 hours . Then flush the column with distilled water to elute 3 times the volume of the resin. When the effluent is clarified, discard the eluent and use 3 times the volume of the resin, and an ethanol solution with a volume fraction of 80% is used as the eluent to elute...

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Abstract

The invention relates to the technical field of deep processing of agricultural products, in particular to a method for preparing Vaccinium spp. anthocyanin and a product of the method. The Vacciniumspp. Anthocyanin can be used for improving polydipsia and polyuria of diabetic patients. The method comprises the steps: adopting Vaccinium ashei as a raw material, extracting Vaccinium spp. anthocyanin by using a food-grade acidic ethanol solution, purifying the obtained Vaccinium spp. anthocyanin by using AB-8 macroporous resin column chromatography, performing elution so as to remove pectin, protein and other impurities by using distilled water, performing elution on the adsorbed Vaccinium spp. anthocyanin by using a food-grade ethanol solution. It is determined that the purity of the Vaccinium spp. anthocyanin prepared by using the method can reach 76% or above, and through gavage of diabetic mice, the symptoms of polydipsia and polyuria and related indexes to glucose and lipid metabolism of the mice can be improved effectively; and the method has low cost, high efficiency, safety and reliability, is suitable for mass production, and has good industrial prospects.

Description

technical field [0001] The invention belongs to the technical field of deep processing of agricultural products, and provides a blueberry anthocyanin and its preparation method and its application in improving polydipsia and polyuria in diabetic patients, and lowering blood sugar and urine sugar. Background technique [0002] Diabetes is a chronic disease with a high incidence worldwide, and my country has become the largest country in the world with diabetes patients. Diabetes mellitus is a syndrome of a series of metabolic disorders of sugar, fat, water, protein and electrolytes caused by insufficient insulin secretion or insulin resistance. Clinically, hyperglycemia is the main feature. Urinary and body weight loss and other manifestations, if not controlled in time, will lead to chronic progressive lesions in the kidneys, eyes, nerves and cardiovascular and other parts, and eventually lead to multi-system functional defects and failure of the body. The pathogenesis of d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/62A61P3/10A61P13/00
CPCA61P3/10A61P13/00C07D311/62
Inventor 黄午阳柴智吴寒李大婧刘春泉宋江峰
Owner JIANGSU ACAD OF AGRI SCI
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