Whey protein antioxidant peptides, preparation method thereof and application thereof

A whey protein and anti-oxidant peptide technology, which is applied in peptide preparation methods, chemical instruments and methods, food preparation, etc., can solve the problem of lack of extraction of whey protein anti-oxidative peptides

Inactive Publication Date: 2010-07-14
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] However, there is still a lack of methods for extracting whey protein antioxidant peptides that can be widely used in practice, as well as the amino acid sequence, properties and uses of these whey protein antioxidant peptides. Therefore, the applicant has carried out a lot of experimental research, and finally made the invention

Method used

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  • Whey protein antioxidant peptides, preparation method thereof and application thereof
  • Whey protein antioxidant peptides, preparation method thereof and application thereof
  • Whey protein antioxidant peptides, preparation method thereof and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0158] Embodiment 1: Preparation of whey protein antioxidant peptide

[0159] Weigh whey protein with a protein concentration greater than 80% sold by New Zealand Fonterra (NZMP) under the trade name Whey Protein Concentrate (WPC80) and put it into an enzyme reactor, add deionized water and stir evenly to prepare milk with a concentration of 30mg / mL Albumin solution, the solution was pretreated at 50°C for 15min, then the pH of the solution was adjusted to 7.6 with 1.0mol / L HCl or NaOH, and 2% of the pancreatin produced by Wuxi Xuemei Enzyme Preparation Co., Ltd. was added in terms of whey protein weight. Protease, then control the pH value of the reaction system to be constant, carry out enzymolysis at this temperature, and measure the degree of hydrolysis by pH-stat method until the degree of hydrolysis reaches 18%. The pH was adjusted to 7.0, refrigerated and centrifuged at 8000r / min for 30min, and the supernatant was freeze-dried. 50 It was 5.52mg / mL.

[0160] The macrop...

Embodiment 2

[0165] Embodiment 2: Preparation of whey protein antioxidant peptide

[0166] Carry out in the same manner as Example 1, except that the 2709 alkaline protease produced by Wuxi Xuemei Enzyme Preparation Co., Ltd. is used.

[0167] As a result, the degree of hydrolysis is 25.09%, and the scavenging rate of DPPH free radical is 63.85%.

Embodiment 3

[0168] Embodiment 3: Preparation of whey protein antioxidant peptide

[0169] Carry out in the same manner as Example 1, except that the papain produced by Wuxi Xuemei Enzyme Preparation Co., Ltd. is used.

[0170] As a result, the hydrolysis degree is 4.50%, and the DPPH free radical scavenging rate is 47.84%.

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Abstract

The invention relates to whey protein antioxidant peptides, a preparation method thereof and application thereof. In the method, whey protein, which is a cheese by-product, and an enzymatic preparation undergo enzymatic hydrolysis to form a whey protein antioxidant peptide zymolyte, the zymolyte is subjected to static or dynamic primary purification by macroporous absorption and separation and purification by gel filtration chromatography, preparative high-performance liquid chromatography and analytical high-performance liquid chromatography to give pure whey protein antioxidant peptide products, and a polypeptide amino acid sequence is measured. The product has high digestion resistance, heat resistance, acid resistance and alkali resistance, high storage stability, low molecular weightand specific amino acid sequence, and can effectively remove free radicals, stop oxidation reaction and reduce the generation of oxidation damages, so the products have a wide application prospect inthe field of food, medicaments, feed and cosmetics.

Description

【Technical field】 [0001] The invention belongs to the technical field of bioactive peptides. More specifically, the present invention relates to whey protein antioxidant peptides, their preparation methods and their uses. 【Background technique】 [0002] Traditional synthetic antioxidants, such as BHA, BHT, PG, TBHQ, etc., are often used as additives to prevent food deterioration due to their low cost and high antioxidant activity. However, their application is limited because of their potential harm to human health. Modern free radical theory points out that active oxygen free radicals lead to oxidative degradation of lipids, proteins and DNA, activate carcinogens, inhibit intracellular antioxidant systems and other oxidative damage, which will cause diabetes, cardiovascular disease, neurodegenerative disorders and cancer, etc. The occurrence and development of chronic diseases. Therefore, natural antioxidant substitutes that can safely improve the body's anti-aging abilit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/20C07K1/16C07K7/08A23L1/305A23L1/29A23K1/16A61K38/10A61K8/64A61P39/06A61Q19/08A23L33/00
Inventor 卢蓉蓉孙震沈浥凌玉芳吴慧赵新灿
Owner JIANGNAN UNIV
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