Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of oligomeric proanthocyanidins

A technology of oligomeric proanthocyanidins and proanthocyanidins, which is applied in the direction of organic chemistry, etc., can solve the problems of difficult industrialization and application, large amount of catalyst used, long reaction time, etc., and achieves excellent antioxidant activity, high water solubility and low degree of polymerization. Effect

Inactive Publication Date: 2012-11-28
SOUTHWEST FORESTRY UNIVERSITY
View PDF6 Cites 26 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Above-mentioned these methods owing to have: or use methyl alcohol in reaction system, or reaction time is too long, or use catalyst amount to be too large and unstable, or need to isolate air or high-pressure equipment and cause inconvenience to operate, and the product that obtains needs to carry out safety again. Defects such as performance evaluation are still limited to the stage of laboratory research and use, and it is difficult to carry out industrialization and application
So far, there has been no report on the preparation of proanthocyanidin oligomers using tea polyphenols from food sources

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Take 10g of commercially available pine bark extract and 10g of tea polyphenols dissolved in 1000ml of aqueous hydrochloric acid solution with a concentration of 1% by weight to prepare a mixed solution with a concentration of 20g / L, and react at 70°C for 1 hour. After the reaction, the reaction solution was cooled to room temperature, and adsorbed with Diaion HP-20 macroporous adsorption resin chromatography column, first washed with 2 column volumes of distilled water to remove impurities, and then with 3 column volumes of 50% ethanol aqueous solution For elution, the collected eluate was concentrated under reduced pressure and dried in vacuo to obtain 19.0 g of oligomeric proanthocyanidins from pine bark with a yield of 95.0% and an average degree of polymerization of 2.75.

Embodiment 2

[0026] Take commercially available pine bark extract 10g, dissolve 5g of tea polyphenols in 300ml of aqueous hydrochloric acid solution with a concentration of 2% by weight, prepare a mixed solution with a concentration of 50g / L, and react at 80°C for 1 hour. After the reaction, the reaction solution was cooled to room temperature and adsorbed with Diaion HP-20 macroporous adsorption resin chromatography column, first washed with 2 column volumes of distilled water to remove impurities, and then with 3 column volumes of 70% ethanol aqueous solution For elution, the collected eluent was concentrated under reduced pressure and dried in vacuo to obtain 13.5 g of oligomeric proanthocyanidins from pine bark with a yield of 90% and an average degree of polymerization of 2.95.

Embodiment 3

[0028] Take 10g of commercially available grape seed polyphenols and 10g of tea polyphenols and dissolve them in 250ml of aqueous hydrochloric acid solution with a concentration of 2% by weight to prepare a mixed solution with a concentration of 80g / L and react at 90°C for 0.5 hours. After the reaction, the reaction solution was cooled to room temperature, put on Sephadex LH-20 chromatographic column, first washed with 2 column volumes of distilled water to remove impurities, and then washed with 3 column volumes of 70% ethanol aqueous solution The collected eluate was concentrated under reduced pressure and freeze-dried to obtain 18.5 g of grape seed oligomeric proanthocyanidins, with a yield of 92.5% and an average degree of polymerization of 2.80.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a preparation method of oligomeric proanthocyanidins. The preparation method comprises the following steps of: adding tea polyphenols to a procyanidine plant raw material or an extract thereof; dissolving the procyanidine plant raw material or the extract into an acid aqueous solution according to percentage concentration by weight; carrying out reaction on a mixture for 0.5-5 h at 40-100 G C; adsorbing the solution after reaction by an adsorbing resin; washing the solution after reaction by a distilled water to remove impurities; eluting the solution after reaction by ethanol water; and concentrating and drying the solution after reaction so as to obtain the oligomeric proanthocyanidins. The preparation method of the oligomeric proanthocyanidins, disclosed by the invention, has the advantages of high oligomeric proanthocyanidin content, low average degree of polymerizatoin, high water solubility, high recovery rate, environment friendliness, high efficiency, simplicity and convenience in operation and low cost, can be applied to the fields, such as foods, healthcare foods, drugs, cosmetics, daily chemicals, animal feeds and the like, and is suitable for large-scale industrial production.

Description

technical field [0001] The invention belongs to the field of chemical preparation methods, in particular to a preparation method of plant antioxidants, in particular to a preparation method of oligomeric proanthocyanidins. Background technique [0002] Proanthocyanidins is a general term for a large class of polyphenolic polymers in plants, composed of different numbers of flavan 3-ols such as catechin, epicatechin and other units through C 4 -C 8 or C 4 -C 6 It is formed by aggregation of different bonding methods such as pine, grapes and other plants, and also exists in many beverages, vegetables, fruits and grains, and is closely related to human daily life. According to the degree of polymerization, two to tetramers are usually called oligomeric proanthocyanidins, and pentamers and above are called polymeric proanthocyanidins. [0003] Studies have shown that the degree of polymerization has a great influence on the antioxidant and other biological activities of proa...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07D311/62
Inventor 赵平张加研姜永新尹继庭姜力华燕
Owner SOUTHWEST FORESTRY UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com