Preparation method of oligomeric proanthocyanidins

A technology of oligomeric proanthocyanidins and proanthocyanidins, which is applied in the direction of organic chemistry, etc., can solve the problems of difficult industrialization and application, large amount of catalyst used, long reaction time, etc., and achieves excellent antioxidant activity, high water solubility and low degree of polymerization. Effect

Inactive Publication Date: 2012-11-28
SOUTHWEST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Above-mentioned these methods owing to have: or use methyl alcohol in reaction system, or reaction time is too long, or use catalyst amount to be too large and unstable, or need to isolate air or high-pressure equipment and cause inconvenience to operate, and the product that obtains needs to carry out

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0023] Example 1:

[0024] Take 10g of commercially available pine bark extract and 10g of tea polyphenols and dissolve them in 1000ml of 1% hydrochloric acid aqueous solution with a concentration of 1% by weight to prepare a mixture with a concentration of 20g / L, and react at 70°C for 1 hour. After the reaction is over, the reaction solution is cooled to room temperature and adsorbed on a Diaion HP-20 macroporous adsorption resin chromatography column. First wash with 2 column volumes of distilled water to remove impurities, and then use 3 column volumes of 50% ethanol aqueous solution. For elution, the collected eluate was concentrated under reduced pressure and vacuum dried to obtain 19.0 g of pine bark oligomeric proanthocyanidins, with a yield of 95.0% and an average degree of polymerization of 2.75.

Example Embodiment

[0025] Example 2:

[0026] Take 10 g of commercially available pine bark extract and 5 g of tea polyphenols and dissolve them in 300 ml of a 2% hydrochloric acid aqueous solution with a concentration of 2% by weight, prepare a mixture with a concentration of 50 g / L, and react at 80° C. for 1 hour. After the reaction, the reaction solution was cooled to room temperature and adsorbed on a Diaion HP-20 macroporous adsorption resin chromatography column, first washed with 2 column volumes of distilled water to remove impurities, and then used 3 column volumes of 70% ethanol aqueous solution For elution, the collected eluate was concentrated under reduced pressure and vacuum dried to obtain 13.5 g of pine bark oligomeric proanthocyanidin, with a yield of 90% and an average degree of polymerization of 2.95.

Example Embodiment

[0027] Example 3:

[0028] Take 10 g of commercially available grape seed polyphenols and 10 g of tea polyphenols and dissolve them in 250 ml of a 2% hydrochloric acid aqueous solution with a concentration of 2% by weight to prepare a mixed solution with a concentration of 80 g / L, and react at 90°C for 0.5 hours. After the reaction, the reaction solution was cooled to room temperature and loaded on the Sephadex LH-20 chromatography column, first washed with 2 column volumes of distilled water to remove impurities, and then washed with 3 column volumes of 70% ethanol aqueous solution. After removing, the collected eluate was concentrated under reduced pressure and freeze-dried to obtain 18.5 g of grape seed oligomeric proanthocyanidins, with a yield of 92.5% and an average degree of polymerization of 2.80.

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Abstract

The invention discloses a preparation method of oligomeric proanthocyanidins. The preparation method comprises the following steps of: adding tea polyphenols to a procyanidine plant raw material or an extract thereof; dissolving the procyanidine plant raw material or the extract into an acid aqueous solution according to percentage concentration by weight; carrying out reaction on a mixture for 0.5-5 h at 40-100 G C; adsorbing the solution after reaction by an adsorbing resin; washing the solution after reaction by a distilled water to remove impurities; eluting the solution after reaction by ethanol water; and concentrating and drying the solution after reaction so as to obtain the oligomeric proanthocyanidins. The preparation method of the oligomeric proanthocyanidins, disclosed by the invention, has the advantages of high oligomeric proanthocyanidin content, low average degree of polymerizatoin, high water solubility, high recovery rate, environment friendliness, high efficiency, simplicity and convenience in operation and low cost, can be applied to the fields, such as foods, healthcare foods, drugs, cosmetics, daily chemicals, animal feeds and the like, and is suitable for large-scale industrial production.

Description

technical field [0001] The invention belongs to the field of chemical preparation methods, in particular to a preparation method of plant antioxidants, in particular to a preparation method of oligomeric proanthocyanidins. Background technique [0002] Proanthocyanidins is a general term for a large class of polyphenolic polymers in plants, composed of different numbers of flavan 3-ols such as catechin, epicatechin and other units through C 4 -C 8 or C 4 -C 6 It is formed by aggregation of different bonding methods such as pine, grapes and other plants, and also exists in many beverages, vegetables, fruits and grains, and is closely related to human daily life. According to the degree of polymerization, two to tetramers are usually called oligomeric proanthocyanidins, and pentamers and above are called polymeric proanthocyanidins. [0003] Studies have shown that the degree of polymerization has a great influence on the antioxidant and other biological activities of proa...

Claims

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Application Information

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IPC IPC(8): C07D311/62
Inventor 赵平张加研姜永新尹继庭姜力华燕
Owner SOUTHWEST FORESTRY UNIVERSITY
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