Whey protein active peptide with antioxidant activity and preparation method thereof
A whey protein, anti-oxidation technology, applied in food preparation, hydrolyzed protein ingredients, cosmetic preparations, etc., can solve the problems of weak antioxidant activity and low content of antioxidant active peptides, and achieve good antioxidant activity, Consistent quality, easy-to-control effects
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Embodiment 1
[0054] 1. Enzyme hydrolysis:
[0055] (1) Whey Protein Dissolved
[0056] Add 1000mL water into the enzymolysis reactor, raise the temperature to 45°C, weigh 40g of whey protein, slowly add it into water under magnetic stirring, adjust the pH to 6.5 with 2M NaOH, and make the whey protein isolate completely under the condition of 45°C Dissolved, the concentration of whey protein in the enzymolysis reactor was 4% (w / v).
[0057] (2) Enzymolysis reaction
[0058] According to the mass percentage of enzyme and substrate (whey protein) being 2.2%, add 0.88g papain, keep the reaction temperature at 45°C, and add 2M NaOH dropwise through the ZDJ-4A automatic potentiometric titrator to maintain the enzymolysis solution The pH6.5 is constant, and the enzymatic hydrolysis time is 3.5 hours.
[0059] 2. Centrifugal
[0060] Heat the enzymolysis solution to 100°C for 10 minutes to inactivate the enzyme, then quickly cool it in an ice bath to room temperature, adjust the enzymolysis s...
Embodiment 2
[0068] 1. Enzyme hydrolysis:
[0069] (1) Whey Protein Dissolved
[0070]Add 1000mL of water into the enzymolysis reactor, raise the temperature to 70°C, weigh 30g of whey protein, slowly add it into water under magnetic stirring, heat for 5min to completely dissolve the whey protein isolate, and then cool down to 40°C. The pH was adjusted to 6.0 with 2M NaOH, and the concentration of whey protein in the enzymolysis reactor was 3% (w / v).
[0071] (2) Enzymolysis reaction
[0072] According to the mass percentage of enzyme and substrate (whey protein) as 1%, add 0.3g papain, keep the reaction temperature at 40°C, and add 2M NaOH dropwise through the ZDJ-4A automatic potentiometric titrator to maintain the enzymolysis solution The pH6.0 is constant, and the enzymatic hydrolysis time is 4 hours.
[0073] 2. Centrifugal
[0074] Heat the enzymatic hydrolysis solution to 90°C for 20 minutes to inactivate the enzyme, then quickly cool it in an ice bath to room temperature, adjus...
Embodiment 3
[0082] 1. Enzyme hydrolysis:
[0083] (1) Whey Protein Dissolved
[0084] Add 1000mL of water into the enzymolysis reactor, raise the temperature to 100°C, weigh 100g of whey protein, add it into water slowly under magnetic stirring, heat for 20min to completely dissolve the whey protein isolate, and then cool down to 60°C. The pH was adjusted to 8.0 with 2M NaOH, and the concentration of whey protein in the enzymolysis reactor was 10% (w / v).
[0085] (2) Enzymolysis reaction
[0086] According to the amount that the mass percent of enzyme and substrate (whey protein) is 6%, add 6g papain, keep reaction temperature to be 60 ℃, and NaOH of 2M is added dropwise by ZDJ-4A automatic potentiometric titrator to maintain the concentration of enzymatic solution. The pH is constant at 8.0, and the enzymatic hydrolysis time is 4 hours.
[0087] 2. Centrifugal
[0088] Heat the enzymatic hydrolysis solution to 95°C for 15 minutes to inactivate the enzyme, then quickly cool it in an i...
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