Plant source composition with antioxidant function
A plant source and composition technology, which is applied in the direction of drug combination, food ingredients as antioxidants, food ingredients functions, etc., can solve difficult problems such as the antioxidant activity of natural active ingredients of plants, so as to promote metabolism and regulate body resistance , strong antioxidant effect
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Embodiment 1
[0029] In this example, Oxygen Radical Absorbance Capacity Assay (ORAC) was used to compare the antioxidant activities of three natural phytochemicals, and the optimal antioxidant concentration ranges of the three phytochemicals were respectively obtained.
[0030] 1.1 Oxygen radical scavenging assay (ORAC) was used to analyze the antioxidant activity of astaxanthin. The results showed that the antioxidant activity of astaxanthin was 1.25μM Trolox equiv. / mg when the concentration of astaxanthin was in the range of 0.5-25μg / mL .
[0031] 1.2 The antioxidant activity of vitamin E was analyzed by oxygen radical scavenging assay (ORAC). The results showed that when the concentration of vitamin E was in the range of 10-100 μg / mL, the antioxidant activity was 0.23 μM Trolox equiv. / mg.
[0032] 1.3 Oxygen radical scavenging assay (ORAC) was used to analyze the antioxidant activity of resveratrol. The results showed that when the concentration of resveratrol was in the range of 0.05-0...
Embodiment 2
[0034]In this example, the oxygen radical scavenging assay (Oxygen Radical Absorbance Capacity Assay, ORAC) was used to coordinate the three phytochemicals to optimize the best combination of their antioxidant effects.
[0035] 2.1 Using the oxygen radical scavenging assay (ORAC), the orthogonal test was carried out on the antioxidant activity of astaxanthin, vitamin E and resveratrol, and 11 combinations of different concentrations were set. The results showed that: astaxanthin: vitamin E: Resveratrol=10:150:1 mass ratio, the maximum antioxidant value of astaxanthin can reach 8.71μM Trolox equiv. / mg, which is 7 times of the antioxidant activity of astaxanthin alone; astaxanthin : Vitamin E: Resveratrol = 50:100:1 mass ratio, the maximum antioxidant value of vitamin E can reach 1.11μM Trolox equiv. / mg, which is 4.8 times the antioxidant activity of vitamin E alone; astaxanthin : Vitamin E: Resveratrol = 200:300:1 mass ratio, can reach the maximum antioxidant value of resveratr...
Embodiment 3
[0038] Using the Oxygen Radical Scavenging Assay (ORAC), the antioxidant activity of astaxanthin, vitamin E and resveratrol alone and their formulations were compared, the results are shown in the table below
[0039]
[0040]
[0041] As shown in the table, the antioxidant activity of the compound astaxanthin:vitamin E:resveratrol is better than the effect of the three antioxidants used alone, and also better than the simple sum of the effects of the three antioxidants, which has a synergistic effect effect. The compound catechin: vitamin E: resveratrol is less effective than resveratrol alone due to the antagonistic effect.
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