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Application of eucheuma gelatinae polysaccharide in preparation of antioxidant skin care products

A kind of technology of Kirin vegetables and skin care products

Inactive Publication Date: 2015-06-24
GUANGZHOU JINAN BIOMEDICINE RES & DEV CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the inconspicuous effect in the anti-oxidation skin care products of the prior art, the defect of poor anti-oxidation effect, the object of the present invention is to provide a kind of purposes of Eucheuma agaricus polysaccharide in the preparation anti-oxidation skin care products, has solved the above defects

Method used

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  • Application of eucheuma gelatinae polysaccharide in preparation of antioxidant skin care products
  • Application of eucheuma gelatinae polysaccharide in preparation of antioxidant skin care products
  • Application of eucheuma gelatinae polysaccharide in preparation of antioxidant skin care products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1, the preparation of Eucheuma agaricus polysaccharide of the present invention

[0033] (1) take a certain amount of Eucheuma qilin, and the washed Eucheuma qilin is pulverized with a pulverizer when the water content of Eucheuma qilin is no more than 8%;

[0034] (2) Soak the pulverized Eucheuma agaricus for 0.5h, and then use a heating reflux device to extract polysaccharides in hot water for 3h;

[0035] (3) Collect the filtrate after cooling to 35-45°C, and concentrate the filtrate to 2 / 3 of the volume of the original filtrate with a rotary evaporator;

[0036] (4) Add 3 times the volume of 95% ethanol, and keep stirring, put it in the refrigerator at 4°C for alcohol precipitation for 12 hours, filter it with four layers of gauze, collect the filtrate, and freeze-dry it after the residual alcohol is partially volatilized, and finally obtain the white fibrous The substance is Eucheuma polysaccharide with an average molecular mass ranging from 1,000 to 10...

Embodiment 2

[0037] Embodiment 2, the cytotoxicity test of Eucheuma agaricus polysaccharide of the present invention

[0038] 1. Test material

[0039] The Eucheuma agaricus polysaccharide prepared in Example 1 was prepared into a 1 mg / mL aqueous solution with pure water, and stored at -20°C for later use.

[0040] 2. Test method

[0041] The 1 mg / mL aqueous solution of Eucheuma agaricus polysaccharide was diluted to different concentrations (the initial concentration was 500 μg / mL), and the cytotoxicity of the Eucheuma agaricus polysaccharide was determined by MTT method.

[0042] 3. Test results

[0043] Such as figure 1 As shown, when the Eucheuma agaricus polysaccharide (EGP) concentration reaches 500 μ g / mL, the survival rate of HaCaT cells is 74.85%; figure 2 As shown, the survival rate of HaCaT cells reached 90% when the positive control drug vitamin C was 100 μg / mL; image 3 As shown, when the concentration of hydrogen peroxide reaches 1 μg / mL, the survival rate of HaCaT cell...

Embodiment 3

[0044] Embodiment 3, research experiment on the antioxidant activity of Eucheuma agaricus polysaccharide of the present invention

[0045] 1. Test material

[0046]According to the preparation method of Example 1, the Eucheuma polysaccharide (EGP) of a-D-(1-6)-glucose sulfate with an average molecular weight of 45000 and a monosaccharide sulfate group substitution degree of 2.13 was extracted.

[0047] 2. Test method

[0048] 2.1. With reference to the results of the cytotoxicity test in Example 2, the Euglena polysaccharide of a-D-(1-6)-glucose sulfate was prepared into a dilution with an initial concentration of 240 μg / mL, and the detection test was carried out by flow cytometry. .

[0049] 2.2. The flow detection test method is as follows:

[0050] The HaCaT cells in the logarithmic growth phase were adjusted to a cell density of 2.0×10 with DMEM growth medium. 5 cells / ml, overnight at 37°C. After the cells grow into a monolayer, discard the culture medium, wash once w...

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Abstract

The invention discloses application of eucheuma gelatinae polysaccharide in preparation of antioxidant skin care products. The range of the average molecular mass of the eucheuma gelatinae polysaccharide is 1000-10000, the antioxidant skin care products prepared by adopting the eucheuma gelatinae polysaccharide comprise cleansing gel, essence, emulsion, stoste, toner, facial cream or facial mask, wherein the raw materials of the toner comprise eucheuma gelatinae polysaccharide, hyaluronic acid, D-panthenol, oat, beta-glucan, trehalose, allantoin, rose extract, 1, 2- pentadiol, glycerin, preservative and the balance of water. The eucheuma gelatinae polysaccharide has the advantages of obvious antioxidant function, excellent moisture retention capacity, long shelf time, continuous antioxidant effect duration and the like, the toner is free of toxic or side effects, is quick to absorb, and can effectively prevent wrinkle generation, delay skin aging and make the skin be vigorous and glossy, which is conducive to the promotion and application of the eucheuma gelatinae polysaccharide skin care products.

Description

technical field [0001] The invention belongs to the field of natural product chemistry, and in particular relates to the use of Eucheuma agaricus polysaccharide in preparing anti-oxidation skin care products. Background technique [0002] Oxidation is the biggest threat to skin aging. Unhealthy diet, sun exposure, stress, environmental pollution, etc. can cause free radicals to flood the skin, resulting in oxidative phenomena such as dull complexion and water shortage. Supply, also caused by the surrounding environment and oxygen. We are in an oxidative environment all the time, so we need to pay attention to anti-oxidation in our daily life, both in terms of health and skin care, especially the anti-oxidation effect of the skin, which requires people's attention. [0003] Reactive oxygen species (ROS) is a general term for a class of reactive oxygen-containing compounds in organisms. It mainly includes superoxide anion, hydroxyl radical and hydrogen peroxide. Under resti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/73A61Q19/08C08B37/00
Inventor 王一飞王怀玲董栋廖晓凤袁晓王巧利
Owner GUANGZHOU JINAN BIOMEDICINE RES & DEV CENT
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