Bimodal microscopic imaging system and imaging method thereof

A micro-imaging, dual-modality technology, applied in the field of micro-imaging, can solve problems such as low phototoxicity

Active Publication Date: 2021-11-26
PEKING UNIV
View PDF6 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the above existing problems in the prior art, the present invention proposes a dual-mode microscopic imaging system and its imaging method combining two-photon fluorescence and optical diffraction tomography. Through the synchronous control of the control subsystem, the optical diffraction layer is used to The characteristics of label-free, non-invasive, and low phototoxicity of analytical technology solve the problems encountered in two-photon fluorescence imagi

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bimodal microscopic imaging system and imaging method thereof
  • Bimodal microscopic imaging system and imaging method thereof
  • Bimodal microscopic imaging system and imaging method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0037] The present invention will be further elaborated below through specific embodiments in conjunction with the accompanying drawings.

[0038] Such as figure 1 with 2 As shown, the dual-mode microscopic imaging system combining two-photon fluorescence and optical diffraction tomography in this embodiment includes: an optical diffraction tomography subsystem, a two-photon fluorescence subsystem, a first dichromatic mirror 115, and a second objective lens 114 and control subsystems;

[0039] The optical diffraction tomography subsystem includes: a first light source 101, a first acousto-optic modulator 102, a first half-wave plate 103, a first polarizing beam splitter 104, a first fiber coupler 105, a second fiber coupler 106, The first polarization-maintaining single-mode fiber 107, the second polarization-maintaining single-mode fiber 108, the scanning lens 111, the first biaxial scanning galvanometer 110, the first objective lens 112, the first camera 130, the first sle...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a bimodal microscopic imaging system and an imaging method thereof. According to the bimodal microscopic imaging system combining two-photon fluorescence and optical diffraction tomography, synchronous control is achieved through the control subsystem, and the problems encountered by two-photon fluorescence imaging are solved by means of the characteristics of no mark, no invasion and small phototoxicity of optical diffraction tomography; meanwhile, a result in diffraction chromatography is calibrated by utilizing two-photon fluorescence, so that a biological sample is imaged from morphological and chemical specificity, and the two-photon fluorescence imaging device is simple and can realize relatively high resolution without a complicated imaging light path; the two modalities are fused based on the control subsystem, and parallel imaging characterization of local specificity and global morphology of the sample is achieved; and according to the invention, the three-dimensional high-resolution refractive index distribution of the to-be-detected sample can be recovered through optical diffraction tomography, and co-positioning is carried out on the two-photon fluorescence imaging of the to-be-detected sample at the same time, so that bimodal microscopic imaging is realized.

Description

technical field [0001] The invention relates to the field of micro-imaging technology, in particular to a dual-mode micro-imaging system combined with two-photon fluorescence and optical diffraction tomography and an imaging method thereof. Background technique [0002] Optical microscopy is an important invention in human history. After four centuries of history, the field of microscopic imaging has been continuously expanding, and different methods have emerged in an endless stream. Microscopic imaging technology uses the interaction between light and matter to magnify tiny objects that are difficult to observe, thereby presenting a microscopic scene that can be distinguished by the human eye. Especially in the field of biomedicine, optical microscopy technology, as an indispensable means of observation, reveals the process of life and promotes human exploration of life science. In order to be able to explain the life process in the microscopic field, the imaging capabili...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N21/01G01N21/64G01N21/45G01N21/84
CPCG01N21/01G01N21/6402G01N21/45G01N21/84
Inventor 施可彬穆书奇董大山杨宏龚旗煌
Owner PEKING UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap