Ribosome rRNA (ribosomal Ribonucleic Acid) two-photon fluorescent probe-quaternary ammonium salt terpyridine derivative as well as preparation method and application thereof

Abstract

The invention discloses a ribosome rRNA two-photon fluorescent probe-quaternary ammonium salt terpyridine derivative as well as a preparation method and application thereof. The structural formula of the ribosome rRNA two-photon fluorescent probe-quaternary ammonium salt terpyridine derivative is as shown in the description. A target product disclosed by the invention can identify RNA by two-photon fluorescent enhancement and realizes a two-photon fluorescent enhancement identification effect; and low-energy long wave (710 nm) excitation has little damage to a living body. The rRNA in cell nucleolus is specifically identified. The colocalization effect of the target product disclosed by the invention and nucleolus Syto9 at the cell nucleolus is good, and a Pearson coefficient can reach 90 percent.

Description

1. Technical field [0001] The invention relates to a ribosome rRNA fluorescent probe, in particular to a ribosome rRNA two-photon fluorescent probe-quaternary ammonium salt terpyridine derivative and its preparation method and application. 2. Background technology [0002] Recently, German biochemist Thomas Carell proposed in the journal Science that RNA is the first self-replicating molecule that forms all life and can be used as the origin of life. RNA molecules include transfer RNA (tRNA), messenger RNA (mRNA) and ribosomal RNA (rRNA), which have many functions, such as transport in vivo, interpretation of genetic information, regulation of gene expression, and some necessary biocatalysis. Among them, rRNA is the most abundant RNA in the cell, and it can combine with protein in the nucleolus to produce ribosomes. With the help of rRNA fluorescent probes, the presence and activity of rRNA in vivo can be observed under a confocal microscope. However, many molecules have p...

AI Technical Summary

Problems solved by technology

However, many molecules have poor permeability to the nuclear membrane and are difficult to enter the nucleus, so there are not many reports on rRNA probes

Currently, the only RNA probes available on the market are the SYTO series of RNA probes. Since the molecular structure of the SYTO probes has not been disclosed, further research on the SYTO probes has been greatly limited.

[0003] In 2006, Qian Li and Young-Tae Chang of New York University used three pyridinium salts to recognize RNA in living cells. In the same year, David Parker's research group at Durham University used Eu complexes to specifically recognize nucleoli, but the above RNA probes are single-photon fluorescent probes

The probes mentioned above all recognize RNA in the nucleolus and cytoplasm of the cell at the same time, and cannot specifically recognize rRNA, mRNA or tRNA

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